Results 1 to 10 of about 3,587,617 (298)
N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations
iScienceSummary: RNA polymerase II (Pol II) has a C-terminal domain (CTD) that is unstructured, consisting of a large number of heptad repeats, and whose precise function remains unclear.
Adam Callan-Sidat +4 more
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Nature Communications, 2018 The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genetics ...
Josh Tycko +9 more
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Engineered gene networks enable non-genetic drug resistance and enhanced cellular robustness
Engineering Biology, 2019 Drug resistance complicates the treatment of cancer and infectious diseases, and often arises from the elevated expression of a gene that neutralises or reduces drug activity.
Brendan Camellato +6 more
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STAR ProtocolsSummary: Here, we present a protocol for encapsulating DNA molecules under crowded conditions within cell-sized lipid-coated droplets. We describe steps for preparing a lipid-oil mixture and adding an aqueous solution containing DNA, which, when mixed ...
Mehdi Shafiei Aporvari +2 more
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High Voltage, 2021 In this work, the air dielectric barrier discharge plasma was applied to a model of protein human serum albumin to explore plasma‐mediated protein damage from a biomolecular perspective.
Siqi Peng +6 more
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Large-scale-free network organisation is likely key for biofilm phase transition
Engineering Biology, 2019 Non-linear Kuramoto model has been used to study synchronised or sync behaviour in numerous fields; however, its application in biology is scarce. Here, the basic model has been introduced and examples where large-scale small-world or scale-free networks
Kumar Selvarajoo
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eLife, 2020 Glutamate transporters are essential players in glutamatergic neurotransmission in the brain, where they maintain extracellular glutamate below cytotoxic levels and allow for rounds of transmission.
Xiaoyu Wang, Olga Boudker
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Towards engineering and production of artificial spider silk using tools of synthetic biology
Engineering Biology, 2020 Spider silk is one of the strongest biomaterials available in nature. Its mechanical properties make it a good candidate for applications in various fields ranging from protective armour to bandages for wound dressing to coatings for medical implants ...
Hashwardhan Poddar +3 more
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Long-range order and short-range disorder in Saccharomyces cerevisiae biofilm
Engineering Biology, 2019 Biofilm, a colony forming cooperative response of microorganisms under environmental stress, is a major concern for food safety, water safety and drug resistance. Most current works focus on controlling biofilm growth by targeting single genes. Here, the
Vincent Piras +2 more
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Mechanistic modelling of a recombinase-based two-input temporal logic gate
Engineering Biology, 2017 Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Jack E. Bowyer +3 more
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