Hachimoji DNA and RNA: A genetic system with eight building blocks [PDF]
, 2019 Reported here are DNA and RNA-like systems built from eight (hachi-) nucleotide letters (-moji) that form four orthogonal pairs. This synthetic genetic biopolymer meets the structural requirements needed to support Darwinism, including a polyelectrolyte ...
Bates, Alison M. +15 more
core +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Short-Chained Oligo(Ethylene Oxide)-Functionalized Gold Nanoparticles: Realization Of Significant Protein Resistance [PDF]
, 2017 Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to ...
Riley, Kathryn R. +4 more
core +2 more sources
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
Biologics: Targets & Therapy, 2021 Misganaw Asmamaw Mengstie, 1 Belay Zawdie Wondimu 2 1Division of Biochemistry, Department of Biomedical Sciences, College of Medicine and Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia; 2Division of Biochemistry, Department of ...
Asmamaw M, Zawdie B
doaj
Compost and fertilizer mineralization effects on soil and harvest in parkland agroforestry systems in the south-Sudanese zone of Burkina Faso [PDF]
, 2007 Decomposition of, and the subsequent nutrient release from tree leaf litter as well as external inputs of nutrients are important in the traditional agroforestry parklands of West Africa. The management of nutrient resources is essential to optimize crop
Gnankambary, Zacharia
core
Modeling, Simulating, and Parameter Fitting of Biochemical Kinetic Experiments
, 2015 In many chemical and biological applications, systems of differential equations containing unknown parameters are used to explain empirical observations and experimental data.
Goulet, D.
core +1 more source
Pathogenic mutations in the hydrophobic core of the human prion protein can promote structural instability and misfolding [PDF]
, 2010 Transmissible spongiform encephalopathies, or prion diseases, are caused by misfolding and aggregation of the prion protein PrP. These diseases can be hereditary in humans and four of the many disease-associated missense mutants of PrP are in the ...
Daggett, Valerie, van der Kamp, Marc W
core +2 more sources
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Microbial Pyrrolnitrin: Natural Metabolite with Immense Practical Utility
Biomolecules, 2019 Pyrrolnitrin (PRN) is a microbial pyrrole halometabolite of immense antimicrobial significance for agricultural, pharmaceutical and industrial implications.
Shraddha Pawar +4 more
doaj +1 more source