Results 21 to 30 of about 9,219 (186)

A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization

Research, 2018
The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine, while the dilemma of metal catalysis, uneasy premodification, and limited reaction ...
Xianglong Hu, Xueqian Zhao, Benzhao He, Zheng Zhao, Zheng Zheng, Pengfei Zhang, Xiujuan Shi, Ryan T. K. Kwok, Jacky W. Y. Lam, Anjun Qin, Ben Zhong Tang   +10 more
doaj   +1 more source

Antibody–Drug Conjugates: The Last Decade

Pharmaceuticals, 2020
An armed antibody (antibody–drug conjugate or ADC) is a vectorized chemotherapy, which results from the grafting of a cytotoxic agent onto a monoclonal antibody via a judiciously constructed spacer arm.
Nicolas Joubert, Alain Beck, Charles Dumontet, Caroline Denevault-Sabourin   +3 more
doaj   +1 more source

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Frontiers in Materials, 2019
The cell membrane is an organized and fluid structure that modulates cellular activities in response to specific extracellular signals, and maintains the critical communication, integration, and homeostasis between the cytosol and the extracellular ...
Chun-Jen Huang, Chun-Jen Huang, Ying-Chih Chang, Ying-Chih Chang   +3 more
doaj   +1 more source

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

Frontiers in Bioengineering and Biotechnology, 2022
LFIA is one of the most successful analytical methods for various target molecules detection. As a recent example, LFIA tests have played an important role in mitigating the effects of the global pandemic with SARS-COV-2, due to their ability to rapidly ...
Andreea-Cristina Mirica, Andreea-Cristina Mirica, Dana Stan, Ioana-Cristina Chelcea, Carmen Marinela Mihailescu, Carmen Marinela Mihailescu, Augustin Ofiteru, Lorena-Andreea Bocancia-Mateescu   +7 more
doaj   +1 more source

Activity Improvement and Thermal Stability Enhancement of D-Aminoacylase Using Protein-Polymer Conjugates

Cumhuriyet Science Journal, 2022
In this study, the synthesis of new polymer-protein conjugates using a grafting-from strategy was performed by employing photo-induced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization.
Cemil Alkan, Nazan Gökşen Tosun, Sema Bilgin, Seçil Erden Tayhan, Esra Koç   +4 more
doaj   +1 more source

Covalent Protein Inhibitors via Tyrosine and Tryptophan Conjugation with Cyclic Imine Mannich Electrophiles

Angewandte Chemie, EarlyView.
Targeted covalent inhibitors (TCI) containing cyclic imine warheads react with tyrosine and tryptophan residues via the Mannich reaction. These cyclic imine warheads show comparable reaction kinetics to cysteine‐reactive electrophiles commonly used in TCIs.
Sijie Wang, Lei Wang, Marco Hadisurya, Siavash Shahbazi Nia, W. Andy Tao, Emily C. Dykhuizen, Casey J. Krusemark   +6 more
wiley   +2 more sources

Beyond phage display: Non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic and bioconjugation scaffold

Frontiers in Microbiology, 2015
For the past twenty-five years, phage display technology has been an invaluable tool for studies of protein-protein interactions. However, the inherent biological, biochemical and biophysical properties of filamentous bacteriophage, as well as the ease ...
Kevin Alexander Henry, Mehdi eArbabi-Ghahroudi, Mehdi eArbabi-Ghahroudi, Mehdi eArbabi-Ghahroudi, Jamie Kathleen Scott, Jamie Kathleen Scott   +5 more
doaj   +1 more source

Multicyclic D‐Stereospecific Hydrolase Dimer With High Sustained Activity

Angewandte Chemie, EarlyView.
Protein macrocyclization was applied to a thermally unstable D‐stereospecific hydrolase using in situ cyclization of proteins (INCYPRO). This site‐specific cross‐linking approach enhanced the resistance of the enzyme to heat and cosolvents.
Anissa Haim, Sandra Liebscher, Rasmus Klintrot, Lorenzo Vallino, Marcelo Masman, Andreas H. Simon, Marianne Hahn, Sven Hennig, Saskia Neubacher, Frank Bordusa, Tom N. Grossmann   +10 more
wiley   +2 more sources

Extended Fused Carbazole‐BODIPY, High Brightness NIR Organic Dyes

Angewandte Chemie, EarlyView.
Highly fused boron‐dipyrromethene (BODIPY) with a strong donor group has been synthesized, and their photophysical behaviors fully characterized. They exhibit outstanding brightness for fluorophores emitting in the NIR region. Water‐soluble nanoparticles filled with one of these dyes demonstrate high efficiency as the NIR II visualization agent ...
Fabien Ceugniet, Valentin Ott, Pascal Retailleau, Yann Bretonniere, Olivier Maury, Manon H. E. Bousquet, Lucie Sancey, Nicolas Leclerc, Denis Jacquemin, Gilles Ulrich   +9 more
wiley   +2 more sources

Tyrosine bioconjugation using stably preparable urazole radicals

Tetrahedron Chem
Our ex situ tyrosine bioconjugation method utilizes the oxidation of N-methylurazole by Bobbitt's salt or electrochemical activation to generate stable radicals for selective tyrosine labeling, thereby addressing the longstanding challenges in protein ...
Shinichi Sato, Shogo Miyano, Keita Nakane, Zhengyi Liu, Munehiro Kumashiro, Tomohide Saio, Yuya Tanaka, Akira Shigenaga, Chizu Fujimura, Eri Koyanagi, Hafumi Nishi, Shusuke Tomoshige, Minoru Ishikawa   +12 more
doaj   +1 more source