Results 11 to 20 of about 156,806 (268)

Bioenergy.

open access: yes, 2011
Bioenergy has a significant greenhouse gas (GHG) mitigation potential, provided that the resources are developed sustainably and that efficient bioenergy systems are used. Certain current systems and key future options including perennial cropping systems, use of biomass residues and wastes and advanced conversion systems are able to deliver 80 to 90 ...
Helena Chum   +23 more
  +10 more sources

A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

open access: yesBiotechnology for Biofuels, 2019
Background Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established.
Yan Liang   +10 more
doaj   +1 more source

Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
Background Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced ...
Chien-Yuan Lin   +15 more
doaj   +1 more source

Engineering isoprenoids production in metabolically versatile microbial host Pseudomonas putida

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
With the increasing need for microbial bioproduction to replace petrochemicals, it is critical to develop a new industrial microbial workhorse that improves the conversion of lignocellulosic carbon to biofuels and bioproducts in an economically feasible ...
Xi Wang   +5 more
doaj   +1 more source

Engineering microbial biofuel tolerance and export using efflux pumps

open access: yesMolecular Systems Biology, 2011
Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade‐off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production ...
Mary J Dunlop   +7 more
doaj   +1 more source

Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction

open access: yesMicrobiome, 2022
Background Plant cell walls are interwoven structures recalcitrant to degradation. Native and adapted microbiomes can be particularly effective at plant cell wall deconstruction. Although most understanding of biological cell wall deconstruction has been
Lauren M. Tom   +15 more
doaj   +1 more source

The pGinger Family of Expression Plasmids

open access: yesMicrobiology Spectrum, 2023
The pGinger suite of expression plasmids comprises 43 plasmids that will enable precise constitutive and inducible gene expression in a wide range of Gram-negative bacterial species.
Allison N. Pearson   +7 more
doaj   +1 more source

An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

open access: yesBiotechnology for Biofuels, 2020
Background Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with ...
Brett M. Garabedian   +6 more
doaj   +1 more source

Scalable and automated CRISPR-based strain engineering using droplet microfluidics

open access: yesMicrosystems & Nanoengineering, 2022
We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip. The microfluidic device contains a 10 × 10 element array, and each element contains sets of electrodes for two electric field ...
Kosuke Iwai   +13 more
doaj   +1 more source

JULES-BE:Representation of bioenergy crops and harvesting in the Joint UK Land Environment Simulator vn5.1 [PDF]

open access: yes, 2020
We describe developments to the land surface model JULES, allowing for flexible user-prescribed harvest regimes of various perennial bioenergy crops or natural vegetation types.
Duran-Rojas, Maria Carolina   +5 more
core   +3 more sources

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