Results 21 to 30 of about 74,478 (157)

Jungle Express is a versatile repressor system for tight transcriptional control

open access: yesNature Communications, 2018
Tightly regulated promoters with strong inducibility and scalability are highly desirable for biological applications. Here the authors describe ‘Jungle Express’, a EilR repressor-based broad host system activated by cationic dyes.
Thomas L. Ruegg   +11 more
doaj   +1 more source

Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS)

open access: yesBiotechnology for Biofuels, 2018
Background Producing valuable fuels and chemicals from lignin is a key factor for making lignocellulosic biomass economically feasible; however, significant roadblocks exist due to our lack of detailed understanding of how lignin is enzymatically ...
Kai Deng   +8 more
doaj   +1 more source

A highly active Burkholderia polyketoacyl-CoA thiolase for production of triacetic acid lactone

open access: yesNature Communications
Triacetic acid lactone (TAL) is a versatile platform chemical traditionally biosynthesized via decarboxylative Claisen condensation by 2-pyrone synthase. However, this route is limited by poor efficiency and dependence on malonyl-CoA.
Zilong Wang   +11 more
doaj   +1 more source

Gene targets for engineering osmotolerance in Caldicellulosiruptor bescii

open access: yesBiotechnology for Biofuels, 2020
Background Caldicellulosiruptor bescii, a promising biocatalyst being developed for use in consolidated bioprocessing of lignocellulosic materials to ethanol, grows poorly and has reduced conversion at elevated medium osmolarities.
Kyle B. Sander   +9 more
doaj   +1 more source

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression

open access: yesNature Communications
Infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana has become a foundational technique in plant biology, enabling efficient delivery of transgenes in planta with technical ease, robust signal, and relatively high throughput.
Sophia N. Tang   +16 more
doaj   +1 more source

Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process

open access: yesBMC Research Notes
Objective Conversion of lignocellulosic biomass to renewable biofuels and bio-based chemicals at scale holds great promise for increasing domestic supplies of energy and alternatives to petrochemicals but is contingent upon improvements in biomass ...
Md Tahmid Islam   +12 more
doaj   +1 more source

Bioenergy Perspectives in the EU Regions: Carbon Neutrality Pathway [PDF]

open access: yes, 2023
Bioenergy plays an important role in the climate neutrality targets of the EU. However, the status of bioenergy implementation varies greatly across the EU.
Vakkilainen, Esa   +2 more
core   +1 more source

Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity

open access: yesBiotechnology for Biofuels, 2018
Background Geranylgeranyl reductase (GGR) is a flavin-containing redox enzyme that hydrogenates a variety of unactivated polyprenyl substrates, which are further processed mostly for lipid biosynthesis in archaea or chlorophyll biosynthesis in plants. To
Corey W. Meadows   +8 more
doaj   +1 more source

Multiscale molecular simulations for the solvation of lignin in ionic liquids

open access: yesScientific Reports, 2023
Lignin, the second most abundant biopolymer found in nature, has emerged as a potential source of sustainable fuels, chemicals, and materials. Finding suitable solvents, as well as technologies for efficient and affordable lignin dissolution and ...
Mood Mohan   +3 more
doaj   +1 more source

Author Correction: Jungle Express is a versatile repressor system for tight transcriptional control

open access: yesNature Communications, 2018
In the original version of this Article, an incorrect URL was provided in the Data Availability Statement regarding the deposition of plasmids listed in Supplementary Table 4. The correct URL is https://public-registry.jbei.org/folders/378.
Thomas L. Ruegg   +11 more
doaj   +1 more source

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