Results 21 to 30 of about 82,479 (332)

Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates

Biotechnology for Biofuels, 2019
Background Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents.
Yusuke Sasaki, Thomas Eng, Robin A. Herbert, Jessica Trinh, Yan Chen, Alberto Rodriguez, John Gladden, Blake A. Simmons, Christopher J. Petzold, Aindrila Mukhopadhyay   +9 more
doaj   +1 more source

Planning for increased bioenergy use [PDF]

, 2006
There are several goals aimed at increasing the use of renewable energy in the Swedish energy system. Bioenergy is one important renewable energy source and there is a potential to increase its use in the future.
Jonsson, Anna
core   +1 more source

An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

Biotechnology for Biofuels, 2020
Background Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with ...
Brett M. Garabedian, Corey W. Meadows, Florence Mingardon, Joel M. Guenther, Tristan de Rond, Raya Abourjeily, Taek Soon Lee   +6 more
doaj   +1 more source

Scalable and automated CRISPR-based strain engineering using droplet microfluidics

Microsystems & Nanoengineering, 2022
We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip. The microfluidic device contains a 10 × 10 element array, and each element contains sets of electrodes for two electric field ...
Kosuke Iwai, Maren Wehrs, Megan Garber, Jess Sustarich, Lauren Washburn, Zachary Costello, Peter W. Kim, David Ando, William R. Gaillard, Nathan J. Hillson, Paul D. Adams, Aindrila Mukhopadhyay, Hector Garcia Martin, Anup K. Singh   +13 more
doaj   +1 more source

Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts

Biotechnology for Biofuels, 2017
Background Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilate the major lignocellulose-derived
Junko Yaegashi, James Kirby, Masakazu Ito, Jian Sun, Tanmoy Dutta, Mona Mirsiaghi, Eric R. Sundstrom, Alberto Rodriguez, Edward Baidoo, Deepti Tanjore, Todd Pray, Kenneth Sale, Seema Singh, Jay D. Keasling, Blake A. Simmons, Steven W. Singer, Jon K. Magnuson, Adam P. Arkin, Jeffrey M. Skerker, John M. Gladden   +19 more
doaj   +1 more source

Jungle Express is a versatile repressor system for tight transcriptional control

Nature Communications, 2018
Tightly regulated promoters with strong inducibility and scalability are highly desirable for biological applications. Here the authors describe ‘Jungle Express’, a EilR repressor-based broad host system activated by cationic dyes.
Thomas L. Ruegg, Jose H. Pereira, Joseph C. Chen, Andy DeGiovanni, Pavel Novichkov, Vivek K. Mutalik, Giovani P. Tomaleri, Steven W. Singer, Nathan J. Hillson, Blake A. Simmons, Paul D. Adams, Michael P. Thelen   +11 more
doaj   +1 more source

Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS)

Biotechnology for Biofuels, 2018
Background Producing valuable fuels and chemicals from lignin is a key factor for making lignocellulosic biomass economically feasible; however, significant roadblocks exist due to our lack of detailed understanding of how lignin is enzymatically ...
Kai Deng, Jijiao Zeng, Gang Cheng, Jian Gao, Kenneth L. Sale, Blake A. Simmons, Anup K. Singh, Paul D. Adams, Trent R. Northen   +8 more
doaj   +1 more source

Prophage Activation Triggers Extracellular Synthesis of DNA in Bacterial Biofilms via Rolling Circle Replication

Angewandte Chemie, EarlyView.
Shewanella oneidensis and Bacillus subtilis, both harboring prophages, reveal a novel biosynthetic route to scalable and programmable DNA superstructures in flower‐ and wire‐like shapes, respectively. Activation of prophages is achieved via 3‐hour starvation and monitored using reporter strains.
Gabriel Antonio S. Minero, Line Mørkholt Lund, Lasse Hyldgaard Klausen, Obinna Markraphael Ajunwa, Mingdong Dong, Rikke Louise Meyer, Victoria Birkedal, Kai Thormann   +7 more
wiley   +2 more sources

A highly active Burkholderia polyketoacyl-CoA thiolase for production of triacetic acid lactone

Nature Communications
Triacetic acid lactone (TAL) is a versatile platform chemical traditionally biosynthesized via decarboxylative Claisen condensation by 2-pyrone synthase. However, this route is limited by poor efficiency and dependence on malonyl-CoA.
Zilong Wang, Seokjung Cheong, Jose Henrique Pereira, Weixi Hu, Yifan Guo, Andy DeGiovanni, Guangxu Lan, Jinho Kim, Robert W. Haushalter, Taek Soon Lee, Paul D. Adams, Jay D. Keasling   +11 more
doaj   +1 more source

Multiscale Modelling of the Electrocatalysis of Microenzymes: Insights Into the Performance of Microperoxidase at an Aqueous/Graphene Interface and Their Exploitation to Improve Performance

Advanced Science, EarlyView.
A novel multiscale MD/DFT framework to elucidate microenzyme adsorption and electron transfer is reported. This method reveals distinct configurations for microperoxidase‐11 on graphene and those best suited for electron transfer. Rate constants align with the limited experimental data, establishing this framework as a tool for optimizing microenzyme ...
Milan Mijajlovic, Cheng Hu, Yuhui Sun, Matthew J. Penna, Meisam V. Kiamahalleh, Wenrong Yang, Mark J. Biggs   +6 more
wiley   +1 more source