Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and ...
Taek Soon Lee
exaly +4 more sources
Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways [PDF]
Scientific Reports, 2022 Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals.
Hongjiang Liu +2 more
doaj +4 more sources
Engineering isoprenoids production in metabolically versatile microbial host Pseudomonas putida [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 With the increasing need for microbial bioproduction to replace petrochemicals, it is critical to develop a new industrial microbial workhorse that improves the conversion of lignocellulosic carbon to biofuels and bioproducts in an economically feasible ...
Xi Wang +5 more
doaj +2 more sources
Multidimensional optimization for accelerating light-powered biocatalysis in Rhodopseudomonas palustris [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Whole-cell biocatalysis has been exploited to convert a variety of substrates into high-value bulk or chiral fine chemicals. However, the traditional whole-cell biocatalysis typically utilizes the heterotrophic microbes as the biocatalyst ...
Yang Zhang +5 more
doaj +2 more sources
Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides [PDF]
Biotechnology for Biofuels and BioproductsBackground To strengthen the national energy supply, there is an increasing demand for domestically generated aviation fuels. Bio-derived advanced aviation fuels offer the opportunity to meet this domestic need while presenting a unique opportunity to ...
Valentina E. Garcia +15 more
doaj +2 more sources
A Pseudomonas putida efflux pump acts on short-chain alcohols [PDF]
Biotechnology for Biofuels, 2018 Background The microbial production of biofuels is complicated by a tradeoff between yield and toxicity of many fuels. Efflux pumps enable bacteria to tolerate toxic substances by their removal from the cells while bypassing the periplasm.
Georg Basler +3 more
doaj +4 more sources
Alternate routes to acetate tolerance lead to varied isoprenol production from mixed carbon sources in Pseudomonas putida [PDF]
Applied and Environmental MicrobiologyLignocellulose is a renewable resource for the production of a diverse array of platform chemicals, including the biofuel isoprenol. Although this carbon stream provides a rich source of sugars, other organic compounds, such as acetate, can be used by ...
Guilherme M. V. de Siqueira +8 more
doaj +2 more sources
Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli [PDF]
Metabolic Engineering, 2019 Isoprenol (3-methyl-3-buten-1-ol) is a drop-in biofuel and a precursor for commodity chemicals. Biological production of isoprenol via the mevalonate pathway has been developed and optimized extensively in Escherichia coli, but high ATP requirements and ...
Aram Kang +2 more
exaly +3 more sources
Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida [PDF]
Nature CommunicationsAdvances in genome engineering have improved our ability to perturb microbial metabolic networks, yet bioproduction campaigns often struggle with parsing complex metabolic datasets to efficiently enhance product titers.
David N. Carruthers +15 more
doaj +2 more sources
Origin of replication discovery for environmentally isolated Pantoea strain enables expression of heterologous proteins, pathways and products [PDF]
iScienceSummary: Leveraging predicted origin sequences from a previously characterized groundwater plasmidome, we constructed a barcoded plasmid library to screen for previously unknown origins.
Alex Codik +10 more
doaj +2 more sources