Enhancing Isoprenol Production by Systematically Tuning Metabolic Pathways Using CRISPR Interference in E. Coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and ...
Taek Soon Lee
exaly +10 more sources
Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides [PDF]
Biotechnology for Biofuels and BioproductsBackground To strengthen the national energy supply, there is an increasing demand for domestically generated aviation fuels. Bio-derived advanced aviation fuels offer the opportunity to meet this domestic need while presenting a unique opportunity to ...
Valentina E. Garcia +15 more
doaj +7 more sources
Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida [PDF]
Nature CommunicationsAdvances in genome engineering have improved our ability to perturb microbial metabolic networks, yet bioproduction campaigns often struggle with parsing complex metabolic datasets to efficiently enhance product titers.
David N. Carruthers +15 more
doaj +8 more sources
Separation of Isoprenol-Dimethyldioxane Mixture by Vacuum and Extractive Distillation
International Journal of Chemical EngineeringThis study investigates the isolation of commercial isoprenol, a by-product of large-scale isoprene production. The challenge lies in separating isoprenol from an azeotropic mixture with 4,4-dimethyldioxane-1,3.
Alexander V Malygin +2 more
exaly +4 more sources
Evolutionary Engineering of E. Coli MG1655 for Tolerance Against Isoprenol [PDF]
Biotechnology for Biofuels, 2020 Background Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product.
Jens O Krömer, Heiko Babel
exaly +6 more sources
Alternate routes to acetate tolerance lead to varied isoprenol production from mixed carbon sources in Pseudomonas putida [PDF]
Applied and Environmental MicrobiologyLignocellulose is a renewable resource for the production of a diverse array of platform chemicals, including the biofuel isoprenol. Although this carbon stream provides a rich source of sugars, other organic compounds, such as acetate, can be used by ...
Guilherme M. V. de Siqueira +8 more
doaj +3 more sources
Engineering isoprenoids production in metabolically versatile microbial host Pseudomonas putida [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 With the increasing need for microbial bioproduction to replace petrochemicals, it is critical to develop a new industrial microbial workhorse that improves the conversion of lignocellulosic carbon to biofuels and bioproducts in an economically feasible ...
Xi Wang +5 more
doaj +3 more sources
Vapor–Liquid Equilibria and Chemical Equilibria in the System (Formaldehyde + Water + Isoprenol)
Industrial & Engineering Chemistry Research, 2021 Isoprenol (3-methyl-3-buten-1-ol) is an important intermediate in the chemical industry and is used, for example, in the production of various scent and flavor chemicals. Isoprenol is produced from aqueous formaldehyde and isobutene and is separated by distillation from mixtures containing formaldehyde, water, and isobutene in the process.
Hans Hasse +2 more
exaly +3 more sources
Optimization of the IPP-Bypass Mevalonate Pathway and Fed-Batch Fermentation for the Production of Isoprenol in Escherichia coli [PDF]
Metabolic Engineering, 2019 Isoprenol (3-methyl-3-buten-1-ol) is a drop-in biofuel and a precursor for commodity chemicals. Biological production of isoprenol via the mevalonate pathway has been developed and optimized extensively in Escherichia coli, but high ATP requirements and isopentenyl diphosphate (IPP) toxicity have made it difficult to achieve high titer, yield, and ...
Edward E K Baidoo +2 more
exaly +8 more sources
Genome-Scale and Pathway Engineering for the Sustainable Aviation Fuel Precursor Isoprenol Production in Pseudomonas putida [PDF]
Metabolic EngineeringSustainable aviation fuel (SAF) will significantly impact global warming in the aviation sector, and important SAF targets are emerging. Isoprenol is a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane) and has been produced in several engineered microorganisms.
Taek Soon Lee, Aindrila Mukhopadhyay
exaly +8 more sources