Evolution-Guided Tolerance Engineering of Pseudomonas putida KT2440 for Production of the Aviation Fuel Precursor Isoprenol [PDF]
Metabolic EngineeringIsoprenol (3-methyl-3-buten-1-ol) is a precursor to aviation fuels and other commodity chemicals and can be microbially synthesized from renewable carbon streams. Its production has been demonstrated in Pseudomonas putida KT2440 but its titers, rates, and yields have yet to reach commercially viable levels, potentially due to toxicity to the bacterial ...
Aindrila Mukhopadhyay, Adam M. Feist
exaly +5 more sources
Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i>. [PDF]
Sci AdvABSTRACT Synthetic biology tools have accelerated the generation of simple mutants, but combinatorial testing remains challenging. High-throughput methods struggle translating from proof-of-principle molecules to advanced bioproducts.
Menasalvas J +17 more
europepmc +7 more sources
Engineering Saccharomyces cerevisiae for Isoprenol Production [PDF]
Metabolic Engineering, 2021 Isoprenol (3-methyl-3-butene-1-ol) is a valuable drop-in biofuel and an important precursor of several commodity chemicals. Synthetic microbial systems using the heterologous mevalonate pathway have recently been developed for the production of isoprenol in Escherichia coli, and a significant yield and titer improvement has been achieved through a ...
Edward E K Baidoo +2 more
exaly +5 more sources
Multidimensional optimization for accelerating light-powered biocatalysis in Rhodopseudomonas palustris [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Whole-cell biocatalysis has been exploited to convert a variety of substrates into high-value bulk or chiral fine chemicals. However, the traditional whole-cell biocatalysis typically utilizes the heterotrophic microbes as the biocatalyst ...
Yang Zhang +5 more
doaj +2 more sources
Origin of replication discovery for environmentally isolated Pantoea strain enables expression of heterologous proteins, pathways and products [PDF]
iScienceSummary: Leveraging predicted origin sequences from a previously characterized groundwater plasmidome, we constructed a barcoded plasmid library to screen for previously unknown origins.
Alex Codik +10 more
doaj +2 more sources
Multi-layered metabolic remodeling of Pseudomonas putida for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel [PDF]
Metabolic Engineering CommunicationsIsoprenyl acetate, a volatile ester derived from isoprenol, is a key biosynthetic intermediate for the advanced aviation fuel candidate, 1,4-dimethylcyclooctane.
Chae Won Kang +7 more
doaj +2 more sources
Off-gas capture: a promising strategy for removal and recovery of toxic bioproducts in aerobic fermentation [PDF]
Biotechnology for Biofuels and BioproductsIn many bioprocesses, maximum achievable titers are limited below economically viable levels by toxic accumulation of the primary end-product. To combat end-product inhibition, a variety of in situ product removal technologies have been developed to ...
Carolina Araujo Barcelos +3 more
doaj +2 more sources
Lepidopteran mevalonate pathway optimization in Escherichia coli efficiently produces isoprenol analogs for next-generation biofuels [PDF]
Metabolic Engineering, 2021 Terpenes constitute the largest class of natural products with over 55,000 compounds with versatile applications including drugs and biofuels. Introducing structural modifications to terpenes through metabolic engineering is an efficient and sustainable way to improve their properties.
Bo Pang +8 more
openaire +5 more sources
Quantifying Isoprenoids in the Ergosterol Biosynthesis by Gas Chromatography–Mass Spectrometry [PDF]
Journal of Fungi, 2023 The ergosterol pathway is a promising target for the development of new antifungals since its enzymes are essential for fungal cell growth. Appropriate screening assays are therefore needed that allow the identification of potential inhibitors.
Maximilian Liebl +6 more
doaj +2 more sources
Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways [PDF]
Scientific Reports, 2022 Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals.
Hongjiang Liu +2 more
doaj +2 more sources