Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides [PDF]
Biotechnology for Biofuels and BioproductsBackground To strengthen the national energy supply, there is an increasing demand for domestically generated aviation fuels. Bio-derived advanced aviation fuels offer the opportunity to meet this domestic need while presenting a unique opportunity to ...
Valentina E. Garcia +15 more
doaj +4 more sources
Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and ...
Jinho Kim +3 more
doaj +2 more sources
Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production [PDF]
Metabolic Engineering, 2016 Branched C5 alcohols are promising biofuels with favorable combustion properties. A mevalonate (MVA)-based isoprenoid biosynthetic pathway for C5 alcohols was constructed in Escherichia coli using genes from several organisms, and the pathway was optimized to achieve over 50% theoretical yield.
Aram Kang +2 more
exaly +5 more sources
High-throughput enzyme screening platform for the IPP-bypass mevalonate pathway for isopentenol production [PDF]
Metabolic Engineering, 2017 Isopentenol (or isoprenol, 3-methyl-3-buten-1-ol) is a drop-in biofuel and a precursor for commodity chemicals such as isoprene. Biological production of isopentenol via the mevalonate pathway has been optimized extensively in Escherichia coli, yielding 70% of its theoretical maximum.
Aram Kang, Corey W Meadows, Nicolas Canu
exaly +6 more sources
Microbial production of high octane and high sensitivity olefinic ester biofuels [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Advanced spark ignition engines require high performance fuels with improved resistance to autoignition. Biologically derived olefinic alcohols have arisen as promising blendstock candidates due to favorable octane numbers and synergistic ...
David N. Carruthers +8 more
doaj +2 more sources
Metabolic Engineering, 2019 Isoprenol (3-methyl-3-buten-1-ol) is a drop-in biofuel and a precursor for commodity chemicals. Biological production of isoprenol via the mevalonate pathway has been developed and optimized extensively in Escherichia coli, but high ATP requirements and isopentenyl diphosphate (IPP) toxicity have made it difficult to achieve high titer, yield, and ...
Aram Kang +2 more
exaly +7 more sources
Engineering isoprenoids production in metabolically versatile microbial host Pseudomonas putida
Biotechnology for Biofuels and Bioproducts, 2022 With the increasing need for microbial bioproduction to replace petrochemicals, it is critical to develop a new industrial microbial workhorse that improves the conversion of lignocellulosic carbon to biofuels and bioproducts in an economically feasible ...
Xi Wang +5 more
doaj +1 more source
A mevalonate bypass system facilitates elucidation of plastid biology in malaria parasites.
PLoS Pathogens, 2020 Malaria parasites rely on a plastid organelle for survival during the blood stages of infection. However, the entire organelle is dispensable as long as the isoprenoid precursor, isopentenyl pyrophosphate (IPP), is supplemented in the culture medium.
Russell P Swift +11 more
doaj +1 more source
Molecular Oncology, 2018 Although MEK blockade has been highlighted as a promising antitumor drug, it has poor clinical efficacy in KRAS mutant colorectal cancer (CRC). Several feedback systems have been described in which inhibition of one intracellular pathway leads to ...
Seul‐Ki Cheon +7 more
doaj +1 more source
Multi-layered metabolic remodeling of <i>Pseudomonas putida</i> for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel. [PDF]
Metab Eng CommunKang CW +7 more
europepmc +1 more source