Multiomics Data Collection, Visualization, and Utilization for Guiding Metabolic Engineering [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Biology has changed radically in the past two decades, growing from a purely descriptive science into also a design science. The availability of tools that enable the precise modification of cells, as well as the ability to collect large amounts of ...
Somtirtha Roy +28 more
doaj +2 more sources
Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels [PDF]
Biotechnology for Biofuels, 2013 Abstract Background The isopentenols, including isoprenol and prenol, are excellent alternative fuels. However, they are not compounds largely accumulated in natural organism. The need for the next generation of biofuels with better physical and chemical properties impels us to develop biosynthetic routes for the ...
Zheng, Yanning +10 more
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Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli [PDF]
Nature CommunicationsMicrobial bioproduction using one-carbon (C1) feedstocks has the potential to decarbonize the manufacturing of materials, fuels, and chemicals. Formate is a promising C1 feedstock, and the realization of industrial, formatotrophic platform organisms is a
Aidan E. Cowan +20 more
doaj +2 more sources
Tolerance Characterization and Isoprenol Production of Adapted Escherichia coli in the Presence of Ionic Liquids [PDF]
ACS Sustainable Chemistry & Engineering, 2018 Ionic liquid (IL)-based pretreatment makes lignocellulosic biomass more accessible to enzymes and improves enzymatic digestibility. However, the ILs left in biomass slurry after pretreatment could inhibit activity of enzymes and microbial fermentation. Therefore, it is necessary to develop robust host strains that are IL-tolerant.
Shizeng Wang +8 more
core +8 more sources
Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli [PDF]
Scientific Reports, 2015 AbstractEngineering of efflux pumps is a promising way to improve host’s tolerance to biofuels such as medium-chain alcohols (CmOHs); however, this strategy is restricted by poor understanding of the efflux pumps engaged in extrusion of solvents. In this study, several Escherichia coli mutants of multidrug transporters were evaluated for isoprenol ...
Wang, Chonglong +4 more
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Enhancement of linalool production in Saccharomyces cerevisiae by utilizing isopentenol utilization pathway [PDF]
Microbial Cell Factories, 2022 Background Linalool is a monoterpenoid, also a vital silvichemical with commercial applications in cosmetics, flavoring ingredients, and medicines. Regulation of mevalonate (MVA) pathway metabolic flux is a common strategy to engineer Saccharomyces ...
Yaoyao Zhang +3 more
doaj +2 more sources
Microbial production of high octane and high sensitivity olefinic ester biofuels [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Advanced spark ignition engines require high performance fuels with improved resistance to autoignition. Biologically derived olefinic alcohols have arisen as promising blendstock candidates due to favorable octane numbers and synergistic ...
David N. Carruthers +8 more
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Enhanced isoprenoid production in Escherichia coli cells harboring the archaeal mevalonate pathway [PDF]
Biochemistry and Biophysics ReportsMicrobial production of isoprenoids has attracted interest as a white biotechnology approach that yields valuable compounds, such as medicinal, biofuel, and sustainable industrial materials.
Takuya Takahashi +4 more
doaj +2 more sources
Micro and mesoporous materials based on zeolite Y for the florol synthesis via the prins cyclization of isoprenol [PDF]
Catalysis TodaySe investigaron varios precursores y catalizadores microporosos y mesoporosos modificados con Sn en la ciclación de Prins de isoprenol con isovaleraldehído para la producción del isómero cis deseado de piranol, Florol ®, utilizando carbonato de dimetilo como disolvente en el intervalo de temperatura de 30-60 oC. La mayor selectividad para piranoles, 70%
Pascal Demuth +9 more
core +3 more sources
Engineering of Transmembrane Alkane Monooxygenases to Improve a Key Reaction Step in the Synthesis of Polymer Precursor Tulipalin A [PDF]
Angew Chem Int Ed EnglA biosynthesis route for the polymer precursor tulipalin A starting from isoprenyl acetate is proposed. The alkane monooxygenase AlkB catalyzes the chemoselective terminal hydroxylation of isoprenyl acetate. Rational enzyme engineering further increased the activity of the membrane enzyme.
Nigl A +10 more
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