Results 1 to 10 of about 102,774 (287)

Editorial: Policy and Regulation in Bioengineering and Biotechnology [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Hector Quemada
exaly   +6 more sources

Editorial: Bioengineering and Biotechnology Approaches in Cardiovascular Sciences [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Martin L Tomov, Jianyi Zhang, James Chong   +2 more
exaly   +4 more sources

Biotechnology, Bioengineering and Applications of Bacillus Nattokinase

Biomolecules, 2022
Thrombosis has threatened human health in past decades. Bacillus nattokinase is a potential low-cost thrombolytic drug without side-effects and has been introduced into the consumer market as a functional food or dietary supplement.
Li Yuan, Chen Liangqi, Tang Xiyu, Li Jinyao   +3 more
doaj   +3 more sources

Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates

STAR Protocols, 2023
Summary: Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs).
Noah Stanis, Karam Khateeb, Jasmine Zhou, Ruikang K. Wang, Azadeh Yazdan-Shahmorad   +4 more
doaj   +1 more source

An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments

STAR Protocols, 2023
Summary: Large biosynthetic gene cluster (BGC) cloning is important for discovering natural product-based drugs and remains challenging in high GC content microorganisms (e.g., Actinobacteria). Here, we present an in vitro CRISPR-Cas12a-mediated protocol
Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, Gao-Yi Tan   +10 more
doaj   +1 more source

A protocol to investigate neural coupling of brain oscillations in rodents using in vivo electrophysiological recordings

STAR Protocols, 2023
Summary: Memory processes are highly dependent on a cross-talk between brain regions via synchronized neural oscillations. Here, we present a protocol to perform multi-site electrophysiological recordings in vivo in freely moving rodents to investigate ...
Cong Wang, Peter G. Stratton, Pankaj Sah, Roger Marek   +3 more
doaj   +1 more source

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry

STAR Protocols, 2022
Summary: Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ ...
Zhengyuan Pang, Li Ye
doaj   +1 more source

Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

STAR Protocols, 2023
Summary: The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We
Adele Moatti, Yuheng Cai, Chen Li, Kristen D. Popowski, Ke Cheng, Frances S. Ligler, Alon Greenbaum   +6 more
doaj   +1 more source

Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells

STAR Protocols, 2023
Summary: Paratransgenesis through genetic manipulation of symbiotic or commensal microorganisms has been proposed as an effective and environmentally sound approach for the control of vector-borne diseases, including tick bite-related pathologies, and ...
Lorena Mazuecos, Almudena González-García, José de la Fuente   +2 more
doaj   +1 more source

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow

STAR Protocols, 2022
Summary: Advances in 3D printing technologies allow fabrication of complex structures at micron resolution. Here, we describe two approaches of fabricating self-powered microfluidic devices utilizing 3D printing: PDMS (polydimethylsiloxane)-based ...
Sung Oh Woo, Myungkeun Oh, Yongki Choi
doaj   +1 more source