Results 31 to 40 of about 62,595 (258)

Myogenic Fusogen‐Engineered Lipid Nanoparticles Enhance mRNA Delivery in Skeletal Muscle

Advanced Functional Materials, EarlyView.
This study reports a biomimetic strategy of engineering full‐length Myomaker, a muscle‐specific fusogen, into lipid nanoparticles (LNPs) to harness the native myoblast fusion capability for skeletal muscle mRNA delivery. The resulting Mymk‐LNPs enhance transfection in differentiating myocytes and enable Cre‐mediated reporter activation in injured ...
Fangyu Zhang, Zhisheng Xu, Yaou Duan, Yehui Sun, Shiying Wu, Tao Long, Mateusz Durbacz, Li Zhang, Yufen Xiao, Yu‐Chung Pien, Erick D. Guerrero, Zeru Tian, Xu Wang, Pratima Basak, Sumanta Chatterjee, Ning Liu, Xiaochun Li, Eric N. Olson, Daniel J. Siegwart   +18 more
wiley   +1 more source

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

Advanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian, Xu Wang, Alexis Pacheco Benitez, Erick Guerrero, Yaou Duan, Jingwen Wei, Lukas Farbiak, Daniel J. Siegwart   +7 more
wiley   +1 more source

Lab‐in‐a‐Tube System Integrating Hierarchically Wrinkled Electrodes and DNAzyme‐Functionalized Beads for Diagnosis of Clostridioides difficile Infection

Advanced Materials, EarlyView.
The point‐of‐care diagnosis of Clostridioides difficile infection remains challenging due to reliance on slow culture and amplification‐based methods. A lab‐in‐a‐tube electrochemical biosensor integrating DNAzymes, antifouling magnetic beads, and a hierarchically structured flow cell enables amplification‐ and culture‐free detection directly in stool ...
Survanshu Saxena, Soyeon Lee, Youngeun Choi, Jonathan L'Heureux‐Hache, Jiaying Liu, Sadman Sakib, Sara M. Imani, Marek Smieja, Yingfu Li, Todd Hoare, Leyla Soleymani   +10 more
wiley   +1 more source

Imaging of Red-Shifted Light From Bioluminescent Tumors Using Fluorescence by Unbound Excitation From Luminescence

Frontiers in Bioengineering and Biotechnology, 2019
Early detection of tumors is today a major challenge and requires sensitive imaging methodologies coupled with new efficient probes. In vivo optical bioluminescence imaging has been widely used in the field of preclinical oncology to visualize tumors and
Fabiane Sônego, Sophie Bouccara, Thomas Pons, Nicolas Lequeux, Anne Danckaert, Jean-Yves Tinevez, Israt S. Alam, Spencer L. Shorte, Régis Tournebize   +8 more
doaj   +1 more source

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

Bioengineering, 2022
Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low ...
Moustafa R. Yehia, Tatyana E. Smolyarova, Alexandr V. Shabanov, Ekaterina S. Sushko, Gennady A. Badun, Nadezhda S. Kudryasheva   +5 more
doaj   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source

A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants

Scientific Reports
Bioluminescence monitoring techniques have greatly contributed to revealing a variety of biological regulatory systems in living organisms, including circadian clocks.
Ryohei Ueno, Shogo Ito, Tokitaka Oyama
doaj   +1 more source

ATP-Bioluminescence as a method to evaluated microbiological quality of UHT milk

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2014
New approaches are needed to quickly indicate possible contamination of UHT milk, among them the technique of ATP-Bioluminescence. Therefore, the aim of this study was to compare the results of culture methods with the results of ATP-Bioluminescence ...
A.F. Cunha, A.D. Lage, M.M. Pereira e Araújo, C.F. Abreu, A.R. Tassinari, M.A. Ferraz, K. Davenport, M.M.O.P. Cerqueira   +7 more
doaj   +1 more source

Similarities and Differences in Quorum Sensing-Controlled Bioluminescence between Photobacterium phosphoreum T3 and Vibrio qinghaiensis sp.-Q67

Applied Sciences, 2022
Quorum sensing is a density-dependent mechanism using chemical signal molecules termed autoinducers to regulate diverse biological processes in bacteria, including bioluminescence. However, the correlation between growth and light emission of two typical
Dan Wang, Linming Bai, Shanshan Li, Wei Yan   +3 more
doaj   +1 more source

From Lab to Landscape: Environmental Biohybrid Robotics for Ecological Futures

Advanced Robotics Research, EarlyView.
This Perspective explores environmental biohybrid robotics, integrating living tissues, microorganisms, and insects for operation in real‐world ecosystems. It traces the leap from laboratory experiments to forests, wetlands, and urban environments and discusses key challenges, development pathways, and opportunities for ecological monitoring and ...
Miriam Filippi
wiley   +1 more source