Results 51 to 60 of about 243,368 (266)
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Molecules, 2014 Avidin and avidin-like proteins are widely used in numerous techniques since the avidin-biotin interaction is known to be very robust and reliable. Within this study, we investigated this bond at the molecular level under harsh conditions ranging from ...
Melanie Köhler +6 more
doaj +1 more source
Biophysical Reviews, 2020 My career in science started with my PhD under Professor Darcy Gilmour at the University of Sydney. I quickly learned his iconoclastic ways and completed the degree in early 1969 in just over three years. Professor Charles Birch was the Head of Zoology and had been watching my progress and offered me a position as Lecturer as soon as my PhD was ...
openaire +2 more sources
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
ADP‐ribosylation: An emerging regulator of the epigenome
Molecular Oncology, EarlyView.ADP‐ribosylation has emerged as a dynamic epigenetic signaling mechanism that modifies histones and chromatin‐associated proteins. Through coordinated PARylation and MARylation, it integrates with other histone modifications to regulate chromatin structure, transcription factor activity, and gene expression, influencing genome function and disease ...
Cristel V. Camacho +2 more
wiley +1 more source
Chirality in Optical Trapping and Optical Binding
Photonics, 2015 Optical trapping is a well-established technique that is increasingly used on biological substances and nanostructures. Chirality, the property of objects that differ from their mirror image, is also of significance in such fields, and a subject of much ...
David S. Bradshaw +3 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Viruses, 2020 The management of chronic hepatitis B virus (CHB) infection is an area of massive unmet clinical need worldwide. In spite of the development of powerful nucleoside/nucleotide analogue (NUC) drugs, and the widespread use of immune stimulators such as ...
Ganesh Selvaraj Duraisamy +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Frontiers in PhysiologyThe clitoris is a vital part of the female sexual system, playing a crucial role in sexual satisfaction and overall sexual health. During arousal, the smooth muscle within the corpus cavernosum of the clitoris relaxes, which increases blood flow and ...
Chitaranjan Mahapatra +4 more
doaj +1 more source