Results 81 to 90 of about 461,509 (270)

Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, EarlyView.
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik, Deniz Bakkalci, Alexander J. MacRobert, Umber Cheema   +3 more
wiley   +1 more source

Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization

IUCrJ, 2018
This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA ...
Inokentijs Josts, Stephan Niebling, Yunyun Gao, Matteo Levantino, Henning Tidow, Diana Monteiro   +5 more
doaj   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Communications Biophysics [PDF]

, 1959
Contains reports on two research projects.United States Air Force (Contract AF19(604)-4112)United States National Institute of Neurological Diseases and Blindness, U.S.
Barlow, J. S., Goldstein, M. H., Jr.
core   +4 more sources

From free energy measurements to thermodynamic inference in nonequilibrium small systems

New Journal of Physics, 2015
Fluctuation theorems (FTs), such as the Crooks or Jarzynski equalities (JEs), have become an important tool in single-molecule biophysics where they allow experimentalists to exploit thermal fluctuations and measure free-energy differences from non ...
A Alemany, M Ribezzi-Crivellari, F Ritort   +2 more
doaj   +1 more source

High-Speed Single-Molecule Tracking of CXCL13 in the B-Follicle

Frontiers in Immunology, 2018
Soluble factors are an essential means of communication between cells and their environment. However, many molecules readily interact with extracellular matrix components, giving rise to multiple modes of diffusion.
Helen Miller, Helen Miller, Jason Cosgrove, Jason Cosgrove, Jason Cosgrove, Adam J. M. Wollman, Adam J. M. Wollman, Emily Taylor, Emily Taylor, Zhaokun Zhou, Zhaokun Zhou, Peter J. O’Toole, Peter J. O’Toole, Mark C. Coles, Mark C. Coles, Mark C. Coles, Mark C. Leake, Mark C. Leake   +17 more
doaj   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

A Method Sustaining the Bioelectric, Biophysical, and Bioenergetic Function of Cultured Rabbit Atrial Cells

Frontiers in Physiology, 2017
Culturing atrial cells leads to a loss in their ability to be externally paced at physiological rates and to maintain their shape. We aim to develop a culture method that sustains the shape of atrial cells along with their biophysical and bioenergetic ...
Noa Kirschner Peretz, Sofia Segal, Limor Arbel-Ganon, Ronen Ben Jehuda, Ronen Ben Jehuda, Yuval Shemer, Yuval Shemer, Binyamin Eisen, Binyamin Eisen, Moran Davoodi, Ofer Binah, Ofer Binah, Yael Yaniv   +12 more
doaj   +1 more source

Communications Biophysics [PDF]

, 1957
Contains reports on six research ...
Brown, Robert M., Frishkopf, Lawrence S., Geisler, C. D., Goldstein, M. H., Jr., Keidel, Ursula O., Keidel, W. D., Kiang, Nelson Y-S., Nathan, P., Sandel, T. T.   +8 more
core  

Cervical Spinal Cord Magnetization Transfer Ratio and Its Relationship With Clinical Outcomes in Multiple Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The cervical spinal cord (cSC) is highly relevant to clinical dysfunction in multiple sclerosis (MS) but remains understudied using quantitative magnetic resonance imaging (MRI). We assessed magnetization transfer ratio (MTR), a semi‐quantitative MRI measure sensitive to MS‐related tissue microstructural changes, in the cSC and its ...
Lisa Eunyoung Lee, Julien Cohen‐Adad, Irene M. Vavasour, Melanie Guenette, Katherine Sawicka, Neda Rashidi‐Ranjbar, Nathan Churchill, Akash Chopra, Adelia Adelia, Pierre‐Louis Benveniste, Anthony Traboulsee, Nathalie Arbour, Fabrizio Giuliani, Larry D. Lynd, Scott B. Patten, Alexandre Prat, Alice Schabas, Penelope Smyth, Roger Tam, Yunyan Zhang, Simon J. Graham, Mojgan Hodaie, Anthony Feinstein, Shannon Kolind, Tom A. Schweizer, Jiwon Oh, CanProCo Study Group   +26 more
wiley   +1 more source