Results 131 to 140 of about 42,861 (236)

Imperatives for anaerobic high cell density culturing by denitrification: technical and physiological perspectives. [PDF]

Microb Cell Fact
Maråk MM, Duister IM, Bakken LR, Bergaust LL.   +3 more
europepmc   +1 more source

Hydrogel Embedding of Mesenchymal Stem Cells Supports Extracellular Vesicle Production

Biotechnology and Bioengineering, EarlyView.
This study investigates the effects of 3D culture conditions on extracellular vesicle (EV) production by mesenchymal stem cells (MSCs) in a gelatin‐based hydrogel matrix (GelMA). The EVs from both conditions were found to have similar morphological, molecular, and functional properties, but 3D cultures produced more total EVs per volume.
Rachel K. Moen, Gabriella Faircloth, Nicole T. Marguerite, Mariachiara Conti, Ethan S. Lippmann, Jamey D. Young   +5 more
wiley   +1 more source

Scalable CAR-T production in a 2-litre perfusion stirred-tank bioreactor with automated harvesting and scale-down model characterisation. [PDF]

Front Bioeng Biotechnol
Springuel P, Silva Couto P, Stibbs DJ, Szelwicki M, Frangleton A, Schmidberger T, George A, Slingsby F, Bevan N, Ahmad A, Legmann R, Dianat N, Ladi R, Hengst J, Rafiq QA.   +14 more
europepmc   +1 more source

Engineering of 2‐ketoacid Decarboxylases for Production of Isobutanol and Other Fusel Alcohols in Saccharomyces cerevisiae

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Isobutanol is a fusel alcohol that can be produced microbially for use as a biofuel or upgraded into sustainable aviation fuel (SAF). A key enzyme in the isobutanol biosynthetic pathway is 2‐ketoacid decarboxylase (KDC), which irreversibly decarboxylates 2‐ketoisovalerate (KIV) to yield isobutyraldehyde.
Joshua J. Dietrich, Maelia Dziedzic, Jia Sun, Sri Harsha Adusumilli, Carla Gonçalves, Chris Todd Hittinger, Brian F. Pfleger   +6 more
wiley   +1 more source

Acid adaptation of Lactobacillus helveticus during continuous cultures to improve undissociated lactic acid production. [PDF]

Bioresour Bioprocess
Collet L, Delettre J, Athès V, Pénicaud C, Béal C.   +4 more
europepmc   +1 more source

Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor‐Like Performance

Biotechnology and Bioengineering, EarlyView.
This article presents the design and evaluation of a gas‐control jacket integrated with a breathable shake flask, enabling precise regulation of dissolved oxygen and carbon dioxide to improve scalability, process control, and culture performance in shaking bioreactors.
Vikash Kumar, Chad Sundberg, Venkatesh Srinivasan, Aaron Thole, Michael Tolosa, Govind Rao   +5 more
wiley   +1 more source

Advancing Raman Calibration: Automated Data Generation, Monitoring, and Control in Multi-Parallel Perfusion Mini Bioreactors. [PDF]

Biotechnol J
Machleid R, Goertz M, Grimm C, Trygg J, Mathias S, Surowiec I.   +5 more
europepmc   +1 more source

Influence of Zn2+ and Oxygen Supply on Malic Acid Production and Growth of Aspergillus oryzae

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Malic acid is a valuable platform chemical traditionally derived from fossil‐based resources. Microbial cultivation with Aspergillus oryzae offers a sustainable alternative based on renewable feedstocks. In this study, a well‐established minimal medium for malic acid production, commonly used in previous research to ensure reproducibility, was
Lukas Hartmann, Anke Neumann, Dirk Holtmann, Katrin Ochsenreither   +3 more
wiley   +1 more source

Modeling immunotherapies in live 3D human cancer tissue bioreactors. [PDF]

Theranostics
Zhang Y, Foth I, Makky A, Bucher P, Grimm M, Bruch PM, Hagelstein I, Dietrich S, Leibold J, Flatz L, Feucht J, Becker S, Schürch CM.   +12 more
europepmc   +1 more source

Development of Raman Spectroscopy and Machine Learning Methods for Protein Aggregate Quantification: Application to BSA in Chromatographic Processes

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein aggregation poses a significant risk to biopharmaceutical product quality, as even minor amounts of oligomeric species can compromise efficacy and safety. Rapid and reliable detection of protein aggregates thus remains a major challenge in biopharmaceutical manufacturing.
Jakob Heyer‐Müller, Robin Schiemer, Lars Robbel, Michael Schmitt, Jürgen Hubbuch   +4 more
wiley   +1 more source