Results 161 to 170 of about 6,069,719 (250)

Protocol for controlled gas-phase oxygen In-leakage in anaerobic bioreactors. [PDF]

MethodsX
Shimokawara S, Maeda T.
europepmc   +1 more source

Mechanistic Digital Model for Real‐Time Monitoring and Optimization of Adenovirus Production

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adenoviral vectors are a critical vaccine platform, with billions of vaccine doses administered during the COVID‐19 pandemic. In addition, they are promising therapeutics for cancer treatment. To advance their manufacturing, real‐time monitoring of adenovirus production is essential for process optimization, yield improvement, and product ...
Xingge Xu, Daniel Rüdiger, Jeongbin Shin, Shawninder Chahal, Amine Kamen   +4 more
wiley   +1 more source

Feathered bioreactor: transforming a chicken egg into a recombinant protein biomanufacturing foundry. [PDF]

Poult Sci
Naim A, Sharma A, Ray SK, Nayak D.
europepmc   +1 more source

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

A Scalable Bioreactor Platform for Reproducible Production and Characterization of Ovarian Cancer-Derived Extracellular Vesicles. [PDF]

Bioengineering (Basel)
Fu W, Dorayappan KDP, Hisey C, Chakrapani LN, Wiggins S, Sundaram S, Lambert Z, Nguyen KT, Chelian SA, Reategui E, Selvendiran K, Hansford DJ.   +11 more
europepmc   +1 more source

Scaffolding a Low‐Cost Experiential Bioreactor Module: Reducing Cognitive Load to Enhance Competency and Self‐Efficacy

Biochemistry and Molecular Biology Education, EarlyView.
ABSTRACT Low‐cost experiential modules are critical for democratizing biotechnology education, yet most undergraduates lack hands‐on experience with continuous‐culture bioreactor operations, which are fundamental to industrial bioprocessing. This study employed design‐based research across two iterative cycles (Fall 2024, n = 8; Fall 2025, n = 11) to ...
Russell K. Pirlo, Matthew Lopper, Justin C. Biffinger   +2 more
wiley   +1 more source

Alluvial filtration as a scalable and safe single-use technology for the harvest of viral vectors. [PDF]

Appl Microbiol Biotechnol
Wiesner J, Bauer A, Eisenkraetzer D.
europepmc   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Towards a Scalable Production of <i>Staphylococcus epidermidis</i> Extracellular Vesicles: Integration of Stirred-Tank Bioreactor and Ultrafiltration-Based Recovery. [PDF]

Int J Mol Sci
Presutti G, Bosco F, Chiadò A, Limongi T, Vallino M, Pisano R.   +5 more
europepmc   +1 more source