Results 181 to 190 of about 58,775 (305)

High‐throughput development and scale‐up of recombinant adeno‐associated virus 5 anion‐exchange chromatography for high‐purity capsid separation

Biotechnology Progress, EarlyView.
High‐throughput anion‐exchange chromatography process development enables scalable purification of high‐purity recombinant adeno‐associated virus capsids. Abstract Recombinant adeno‐associated virus (rAAV) production commonly yields a high proportion of empty and partially filled capsids, necessitating downstream purification to enrich full capsids ...
Kevin Vera, Ivan Gajic, Praveen Kusumanchi, Angela Andaluz, Gabrielle Dela Pena, Clifford A. Froelich, Elizabeth Higgins, Jennifer Johnston, Seyed Pouria Motevalian   +8 more
wiley   +1 more source

Sedimentation accelerates mixed-substrate conversion during sequential batch cultivation of a co-culture of sugar-specialist yeast strains. [PDF]

Appl Environ Microbiol
Fecker T, Rossouw M, Pronk JT, van Tatenhove-Pel RJ.   +3 more
europepmc   +1 more source

Bioreactors for cardiac organoids

reservedI bioreattori comprendono molte delle strumentazioni necessarie per creare un ambiente favorevole alla crescita cellulare. Una delle loro applicazioni è la coltura di organoidi, strutture tridimensionali che replicano organi o tessuti nativi. Si
LIGUORO, MARIA FRANCESCA
core  

Regenerative perfusion bioreactor for biologics production with lower COGs

Biotechnology Progress, EarlyView.
Abstract Perfusion bioreactor systems enable intensified bioprocessing with high cell densities and volumetric productivities in monoclonal antibody (mAb) manufacturing. However, their widespread adoption remains constrained by the high consumption of chemically defined cell culture media.
Eric Wynne, Dohyun Park, Hourieh Ahi, Jaeweon Lee, Seongkyu Yoon, Jongyoon Han   +5 more
wiley   +1 more source

From biomechanical mechanisms to clinical reasoning: Deciphering mechanobiological drivers of aortic valve calcification for precision therapy. [PDF]

Mechanobiol Med
Jiang Y, He Y, Jia Q, Chen S, Tan Y, Shao Y.   +5 more
europepmc   +1 more source

Impact of single‐use bioreactor bag materials on Spodoptera frugiperda cell expansion for recombinant adeno‐associated virus manufacturing

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus production for gene therapy applications commonly relies on the Spodoptera frugiperda 9 (Sf9) baculovirus infection platform. The process encompasses cell expansion from flasks through intermediate‐scale bioreactors to large‐scale production.
Min Tae Park, Seyed Pouria Motevalian
wiley   +1 more source

Transcriptomic and Epigenetic Reprogramming in <i>Solanum tuberosum</i> L. Micropropagation: A Systematic Review of Elicitor-Driven Acclimatization and Scalable Biofactories. [PDF]

Int J Mol Sci
Sarchi GAJ, Sarchi SAC, Ortega JIM, Hernández F, Martínez RTS.   +4 more
europepmc   +1 more source

Development and application of a mechanistic model for protein A resolution of a bispecific antibody for process characterization and optimization

Biotechnology Progress, EarlyView.
Abstract Regeneron's bispecific technology, based on a fully human IgG antibody construct, consists of two distinct heavy chains and a common light chain co‐expressed in a single cell. The co‐expression of the two distinct heavy chains, one of which contains a dipeptide substitution that ablates Protein A binding, leads to the formation of three ...
Samantha Wadsworth, James Reilly, John Mattila, Christopher Cowan, Hanne Bak   +4 more
wiley   +1 more source

Resolving host-episymbiont interaction dynamics through continuous cultivation. [PDF]

mSystems
Grossman AS, Weng J, Silverman AD, Bor B.   +3 more
europepmc   +1 more source

Challenges in meeting the mass transfer demands of high‐density perfusion cultures in single‐use bioreactors

Biotechnology Progress, EarlyView.
Abstract Increasing protein therapeutics production by adopting intensified perfusion cultures places high demand on the mass transfer capabilities of single‐use bioreactors (SUB). Here, we compare these capabilities for four SUB designs against worst‐case O2 transfer requirements for supporting Chinese hamster ovary (CHO) perfusion cultures. Impact of
Anita B. Dabek, Anthony M. Beaney, Mauran Mahendra, Mikayla Olin, Anastasia Eftychidou, Joanna D. Kolacz, Natalia Papalamprou, Marissa R. Dickson, Patrick Romann, Colin Jaques   +9 more
wiley   +1 more source