Results 201 to 210 of about 111,398 (363)

Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations

Biotechnology Progress, EarlyView.
Abstract The emergence of new viruses and the spread of existing pathogens necessitate efficient vaccine production methods. The baculovirus expression vector system (BEVS) is an efficient and scalable system for subunit and virus‐like particle vaccine production and gene therapy vectors.
Jort J. Altenburg, Brenda E. Juarez‐Garza, Jelle van Keimpema, Linda van Oosten, Gorben P. Pijlman, Monique M. van Oers, René H. Wijffels, Dirk E. Martens   +7 more
wiley   +1 more source

Improved N‐acetylneuraminic acid bioproduction by optimizing pathway for reducing intermediate accumulation

Food Bioengineering, Volume 1, Issue 3-4, Page 205-211, December 2022., 2022
1. To eliminate the extensively accumulated by‐product N‐acetylglucosamine (GlcNAc), the UDP‐GlcNAc epimerase (NeuC) pathway without GlcNAc production was firstly reconstructed and optimized in Bacillus subtilis, and thereby NeuAc titer increased to 5.9 g/L and the by‐product GlcNAc was eliminated. 2.
Haoyu Guo, Rongzhen Tian, Chenyun Wang, Runzhi Zhao, Xueqin Lv, Long Liu, Yanfeng Liu   +6 more
wiley   +1 more source

Factors Affecting the Performance of Bioreactors

, 1984
Ryuichi Matsuno, Kazuhiro Nakanishi
openalex   +2 more sources

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus

Biotechnology Progress, EarlyView.
Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges in understanding and controlling the relationship between morphology and productivity can limit their application.
Katja Rohr, Bertram Geinitz, Johannes Seiffarth, Aydin Anbarani, Sören Bernauer, Matthias Moch, Julia Tenhaef, Wolfgang Wiechert, Katharina Nöh, Marco Oldiges   +9 more
wiley   +1 more source

Bioreactors for Cultivation of Plant Cells at High Density

, 1988
Hideo Tanaka
openalex   +2 more sources

A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coli

Biotechnology Progress, EarlyView.
Abstract Variable lymphocyte receptors (VLRs) are the antigen receptors of jawless vertebrates such as lamprey. VLRs are of growing biotechnological interest for their ability to bind certain antigenic targets with higher affinity than traditional immunoglobulins.
Elizabeth A. Appelt, James B. Thoden, Candis Dancy, Hannah D. Bachmeier, Seth A. Gehrke, Melanie A. Staffenson, Ivan Rayment, Moriah E. Katt, Hazel M. Holden, Eric V. Shusta   +9 more
wiley   +1 more source

BIOREACTOR FOR A LARGER SCALE HEPATOCYTE IN VITRO PERFUSION

, 1994
Jörg C. Gerlach, Jens Encke, O. Hole, Christian A. Müller, C. J. Ryan, P. Neuhaus   +5 more
openalex   +1 more source

Improving outcomes in intensified processing via optimization of the cell line development workflow

Biotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi, Mary Otto, Corey Kretzmer, Amber Petersen, Channing McLaurin, Jana Mahadevan, Jason Gustin, Trissa Borgschulte, David Razafsky   +8 more
wiley   +1 more source

Design, Prototyping, and Testing of a Novel Flowpath with an Array of Six 3D Matrix Vitvo Bioreactors for the NASA Bioculture System [PDF]

The NASA Bioculture System is an advanced cell culture closed-loop system containing highly automated flowpaths designed to conduct long term biology experiments on ISS with earth remote controllable medium flow, temperature, gas composition, medium ...
Almeida, Eduardo, Juran, Cassandra M., Ma, Kristin R.   +2 more
core   +1 more source

Iterative hybrid model based optimization of rAAV production

Biotechnology Progress, EarlyView.
Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller, Gerald Siegwart, Susanne Heider, Michael Sokolov, Angela Botros, Alexandra Umprecht, Moritz von Stosch, Mariano Nicolas Cruz Bournazou   +7 more
wiley   +1 more source