Results 11 to 20 of about 164,929 (275)

Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells

open access: yesSTAR Protocols, 2023
Summary: Paratransgenesis through genetic manipulation of symbiotic or commensal microorganisms has been proposed as an effective and environmentally sound approach for the control of vector-borne diseases, including tick bite-related pathologies, and ...
Lorena Mazuecos   +2 more
doaj   +1 more source

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow

open access: yesSTAR Protocols, 2022
Summary: Advances in 3D printing technologies allow fabrication of complex structures at micron resolution. Here, we describe two approaches of fabricating self-powered microfluidic devices utilizing 3D printing: PDMS (polydimethylsiloxane)-based ...
Sung Oh Woo, Myungkeun Oh, Yongki Choi
doaj   +1 more source

Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures

open access: yesSTAR Protocols, 2023
Summary: Several pre-clinical models reveal that chronic chimeric antigen receptor (CAR) stimulation drives a dysfunctional state that mimics in vivo failure.
Mehmet Emrah Selli   +3 more
doaj   +1 more source

Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)

open access: yesSTAR Protocols, 2023
Summary: We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films.
Rashi Porwal   +2 more
doaj   +1 more source

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses

open access: yesSTAR Protocols, 2023
Summary: Spatial transcriptomics couples visual spatial markers with gene expression levels, but slide space and cost limit the number of samples that can be processed.
Yoshitaka J. Sei   +2 more
doaj   +1 more source

Enzyme-linked immunosorbent assay to detect surface marker proteins of extracellular vesicles purified from human urine

open access: yesSTAR Protocols, 2023
Summary: The molecular profile of extracellular vesicles released in urine reflects the pathophysiological processes occurring within originating cells located in diverse nephron segments.
Keiichi Takizawa   +2 more
doaj   +1 more source

Using EuGeneCiD and EuGeneCiM computational tools for synthetic biology

open access: yesSTAR Protocols, 2021
Summary: Synthetic biology often relies on the design of genetic circuits, utilizing “bioparts” (modular DNA pieces) to accomplish desired responses to external stimuli.
Wheaton L. Schroeder   +2 more
doaj   +1 more source

Gradient matching methods for computational inference in mechanistic models for systems biology: a review and comparative analysis [PDF]

open access: yes, 2015
Parameter inference in mathematical models of biological pathways, expressed as coupled ordinary differential equations (ODEs), is a challenging problem in contemporary systems biology.
Husmeier, Dirk, Macdonald, Benn
core   +10 more sources

A protocol to execute a lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

open access: yesSTAR Protocols, 2023
Summary: Here, we present a protocol for a miniaturized microfluidic device that enables quantitative tracking of bacterial growth. We describe steps for fabricating a screen-printed electrode, a laser-induced graphene heater, and a microfluidic device ...
Sonal Fande   +6 more
doaj   +1 more source

Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants [PDF]

open access: yes, 2020
Pulsed Electric Fields (PEF) is a promising technology for the gentle and energy efficient disruption of microalgae cells such as Chlorella vulgaris.
Knappert, Justus   +2 more
core   +1 more source

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