Results 31 to 40 of about 164,929 (275)

Protocol for designing and preparing gallium particles using cell membrane encapsulation for applications in melanoma cryoablation therapy

STAR Protocols, 2022
Summary: Liquid metals are increasingly applied in drug delivery, in vivo imaging, and biosensing. Herein, we describe a surface modification strategy, where cell membrane is introduced to encapsulate gallium (Ga) particles.
Xuelin Wang, Xing-Jie Liang, Jie Zhang, Junge Chen, Jing Liu   +4 more
doaj   +1 more source

Building a Bird: Musculoskeletal Modeling and Simulation of Wing-Assisted Incline Running during Avian Ontogeny [PDF]

, 2018
Flapping flight is the most power-demanding mode of locomotion, associated with a suite of anatomical specializations in extant adult birds. In contrast, many developing birds use their forelimbs to negotiate environments long before acquiring “flight ...
Ashley M. Heers, Ashley M. Heers, Jeffery W. Rankin, Jeffery W. Rankin, John R. Hutchinson   +4 more
core   +3 more sources

Integration of in vivo electrophysiology and optogenetics in rodents with PEDOT:PSS neural electrode array

STAR Protocols
Summary: Here, we present a protocol for the fabrication of transparent implantable electrode arrays for integrating optogenetics and electrophysiology.
Young Uk Cho, Ju Young Lee, Ki Jun Yu
doaj   +1 more source

Therapeutic Potential of Mesenchymal Stem Cells for Cancer Therapy [PDF]

, 2020
Mesenchymal stem cells (MSCs) are among the most frequently used cell type for regenerative medicine. A large number of studies have shown the beneficial effects of MSC-based therapies to treat different pathologies, including neurological disorders ...
Capilla González, Vivian, Gauthier, Benoit R., Hmadcha, Abdelkrim, Martín Montalvo, Alejandro, Soria Escoms, Bernat   +4 more
core   +1 more source

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

STAR Protocols
Summary: Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging.
Seungbin Park, Megan Lipton, Yujiao J. Sun, Maria Dadarlat   +3 more
doaj   +1 more source

Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems

STAR Protocols, 2022
Summary: Our recent development of the CRAGE (chassis-independent recombinase-assisted genome engineering) system enables single-step integration of large, complex DNA constructs directly into bacteria genomes across multiple phyla.
Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuni   +2 more
doaj   +1 more source

The Institute for Biotechnology and Bioengineering (IBB) [PDF]

, 2009
The Laboratório Associado Institute for Biotechnology and Bioengineering (IBB) is a research unit aiming to be a strategic infrastructure for the development of the Portuguese R&D and innovation policies in the areas of Biotechnology, Bioengineering ...
Cabral, J. M. S., Ferreira, E. C., Ferreira, Guilherme, Martel, Paulo, Mota, M., Pinto, Henrique Guedes, Reis, R. L.   +6 more
core  

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes

STAR Protocols, 2022
Summary: The plasmid-free CRISPR-Cas9-based genome editing in fungi is a precise and time-saving approach. Here, we present a detailed protocol for genetic manipulation in Penicillium funiculosum, which includes design and synthesis of sgRNA, high ...
Tulika Sinha, Syed Shams Yazdani
doaj   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source