Results 161 to 170 of about 488,335 (365)

Microbiome Single Cell Atlases Generated with a Commercial Instrument

Advanced Science, EarlyView.
Single cell sequencing is valuable for dissecting complex systems and uncovering unique features in individual cell types. However, tools for single cell microbial analysis are limited. Easily Accessible Single microbe sequencing (EASi‐seq), which adapts a commercially available single cell platform for efficient sequencing of single microbial genomes ...
Xiangpeng Li, Linfeng Xu, Benjamin Demaree, Cecilia Noecker, Jordan E. Bisanz, Daniel W. Weisgerber, Cyrus Modavi, Peter J. Turnbaugh, Adam R. Abate   +8 more
wiley   +1 more source

Fluorescence Enhancement of Fluorescent Unnatural Streptavidin by Binding of a Biotin Analogue with Spacer Tail and Its Application to Biotin Sensing

The Scientific World Journal, 2014
We designed a novel molecular biosensing system for the detection of biotin, an important vitamin by the combination of fluorescent unnatural streptavidin with a commercialized biotin-(AC5)2-hydrazide.
Xianwei Zhu, Hiroaki Shinohara
doaj   +1 more source

Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes [PDF]

arXiv, 2015
As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating dye YOYO-1 against minor groove binding dye SYTO-13, using a bespoke multicolor single-molecule fluorescence microscope. We used a full-length ~49 kbp {\lambda} DNA construct
arxiv  

METTL14‐Mediated M6A Modification of LINC01094 Induces Glucose Metabolic Reprogramming in Breast Cancer by Recruiting the PKM2/JMJD5 Complex

Advanced Science, EarlyView.
METTL14/IGF2BP2‐mediated m6A modification drives LINC01094 upregulation in BC. Then, LINC01094 interacts with PKM2 monomers to promote their dimerization, while serving as a flexible scaffold to facilitate the assembly of the PKM2/JMJD5 complex, synergistically stabilizing PKM2 dimers and enhancing their nuclear translocation.
Mengqi Wang, Zhaoxin Gao, Ruinan Zhao, Pan Zhou, Jingxi Chen, Hui Zhang, Yawen Wang, Wenjie Zhu, Peng Gao   +8 more
wiley   +1 more source

Case report: Two siblings with very late onset of holocarboxylase synthase deficiency and a mini-review

Frontiers in Genetics
Holocarboxylase synthase (HCS) deficiency is an extremely rare metabolic disorder typically presenting as severe neonatal metabolic acidosis, lethargy, hypotonia, vomiting, and seizures.
Margaux Gaschignard, Louis Domenach, Delphine Lamireau, Claire Guibet, Sandrine Roche, Emmanuel Richard, Isabelle Redonnet-Vernhet, Isabelle Redonnet-Vernhet, Samir Mesli, Louis Lebreton   +9 more
doaj   +1 more source

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

Beilstein Journal of Nanotechnology, 2019
The level of cancer biomarkers in cells, tissues or body fluids can be used for the prediction of the presence of cancer or can even indicate the stage of the disease.
Seyed Mohammad Mahdi Dadfar, Sylwia Sekula-Neuner, Vanessa Trouillet, Hui-Yu Liu, Ravi Kumar, Annie K. Powell, Michael Hirtz   +6 more
doaj   +1 more source

Oxaloacetate decarboxylase of Archaeoglobus fulgidus: cloning of genes and expression in Escherichia coli [PDF]

, 2018
Archaeoglobus fulgidus harbors three consecutive and one distantly located gene with similarity to the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae (KpOadGAB). The water-soluble carboxyltransferase (AfOadA) and the biotin protein (AfOadC)
Dahinden, Pius, Dimroth, Peter, Pos, Klaas, Taralczak, Malgorzata   +3 more
core  

A CMTM6 Nanobody Overcomes EGFR‐TKI Resistance in Non‐Small Cell Lung Cancer

Advanced Science, EarlyView.
Overcoming EGFR‐TKI resistance remains a critical challenge in NSCLC treatment. This study identifies CMTM6 as a key regulator of EGFR stability and demonstrates that a novel anti‐CMTM6 nanobody disrupts the CMTM6‐EGFR interaction. Targeting CMTM6 restores EGFR degradation, suppresses tumor growth, and confers therapeutic benefit in both CDX and PDX ...
Lu Xia, Jichuan Wang, Hui Xue, Haimeng Li, Qinghua Li, Sen Qin, Chunyu Yu, Yanhua Liu, Yu Gao, Lingyun Li, Sudun Guan, Enrun Zheng, Feiya Suo, Lin He, Yongsheng Wang, Wenling Han, Yongfeng Shang, Yong Geng, Luyang Sun   +18 more
wiley   +1 more source

HIC1 suppresses Tumor Progression and Enhances CD8+ T Cells Infiltration Through Promoting GSDMD‐induced Pyroptosis in Gastric Cancer

Advanced Science, EarlyView.
These findings elucidate the innovative role of HIC1 as a transcriptional activator in GC, driving the initiation of pyroptosis and enhancing CD8+ T cell infiltration, which has certain novelty and creative significance. Collectively, targeting HIC1 can present an appealing immunotherapeutic strategy to improve outcomes in GC patients.
Mengjie Kang, Wenqi Du, Lina Ding, Mengdi Wu, Dongsheng Pei   +4 more
wiley   +1 more source

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source