Results 261 to 270 of about 277,237 (293)

The basicity of biotin

Archives of Biochemistry and Biophysics, 1968
Abstract Transannular interaction between the sulfur atom and the ureido carbonyl group has been suggested as a possible function for the sulfur atom in biotin. The results obtained from PK determinations in both anhydrous formic acid and chloroform solutions, in addition to infrared and proton magnetic resonance spectra, indicate that there is no ...
Charles E. Bowen, Elli Rauscher, Lloyd L. Ingraham   +2 more
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Biotin

2004
Steven W.
Polyak, S., Chapman-Smith, A.
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Coupling of Antibodies with Biotin

1996
The avidin-biotin bond is the strongest known biological interaction between a ligand and a protein (Kd = 1.3 x 10-15 M at pH 5.0) (1). The affinity is so high that the avidin-biotin complex is extremely resistant to any type of denaturing agent (2). Biotin (see Fig.
Rosaria P. Haugland, Wendy W. You
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Biochemistry of Biotin

1964
Publisher Summary This chapter focuses on a host of indirect effects observed in biotin deficiency and offers possible explanations for some of these findings. The discussions primarily deal with the results on certain aspects of biotin in intermediary metabolism. The chapter discusses some of the recent data on the biochemistry of biotin.
Krishnamurti Dakshinamurti, S.P. Mistry
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Biotin and Biotin Analogs in Human Urine: Biotin Accounts for only Half of the Total

The Journal of Nutrition, 1993
In studies using avidin-binding assays to measure the urinary excretion of biotin, biotin is sometimes assumed to be equal to the detectable avidin-binding substances present. High performance liquid chromatography was used to separate avidin-binding substances in human urine, and the chromatographic fractions were assayed for avidin-binding substances
Gary L. Lankford, Donald M. Mock, John Cazin   +2 more
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Biotin-responsive Disorders

2006
Two inherited defects in biotin metabolism are known: holocarboxylase synthetase (HCS) deficiency and biotinidase deficiency. Both lead to deficiency of all biotindependent carboxylases, i.e. to multiple carboxylase deficiency (MCD). In HCS deficiency, the binding of biotin to apocarboxylases is impaired.
Baumgartner, Matthias R, Suormala, T
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Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein

2020
Biotinylated molecules are extensively employed in bioanalytics and biotechnology. The currently available assays for quantification of biotin groups suffer from low sensitivity, low accuracy, or provide highly variable responses for different biotin derivatives.
Elke Oberbichler, Hermann J. Gruber, Günther Knör, Eva Schlögl, Vesa P. Hytönen, Jessica Stangl, Felix Faschinger, Maria Wiesauer   +7 more
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Requirement for Biotin and the Function of Biotin in Cells in Culturea^a

Annals of the New York Academy of Sciences, 1985
The requirement of biotin in the culture medium has been established for HeLa cells, human fibroblasts, baby hamster kidney cells (BHK) as well as polyoma-transformed BHK cells. Growth, viability, biotin content and the activities of biotin-dependent enzymes were used as criteria.
Krishnamurti Dakshinamurti, Lorraine E. Chalifour, Rajinder P. Bhullar   +2 more
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Biotin Deficiency and Biotin Supply

Annals of the New York Academy of Sciences, 1985
Annamária Tôth-Dersi, Annamária Tôth-Dersi, Roland Bitsch, Roland Bitsch, Dieter Hoetzel, Dieter Hoetzel   +5 more
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BIOTIN. VI. SYNTHESIS OF BIOTIN AND epiALLOBIOTIN

The Journal of Organic Chemistry, 1947
W. L. McEWEN, Sidney R. Safir, Louis Dorfman, Merle V. Querry, B. R. Baker, Y. Subbarow, Seymour Bernstein   +6 more
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