Results 71 to 80 of about 381,611 (334)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Biotin Supplementation Ameliorates Murine Colitis by Preventing NF-κB Activation

Cellular and Molecular Gastroenterology and Hepatology, 2019
Background & Aims Biotin is a water-soluble vitamin that is indispensable for human health. Biotin deficiency can cause failure-to-thrive, immunodeficiency, alopecia, dermatitis, and conjunctivitis.
Jonathan Skupsky, Subrata Sabui, M. Hwang, M. Nakasaki, M. Cahalan, H. Said   +5 more
semanticscholar   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Novel Biopesticides Based on Recombinant Avidin for Protection of Crops Against Insect Pests [PDF]

, 2012
Farmers are required to increase their food productivity to meet the demand from a continually enlarging population. A major constraint on meeting this requirement is the damage created by insect pests.
HINCHLIFFE, GARETH
core  

Biotin-Targeted Multifunctional Nanoparticles Encapsulating 10-Hydroxycamptothecin and Apoptin Plasmid for Synergistic Hepatocellular Carcinoma Treatment

, 2021
Hepatocellular carcinoma (HCC) is a common cause of death, and there is a lack of effective treatment methods along with observed drug resistance. As compared to traditional treatments, drug delivery systems have been extensively studied and come with ...
Zhuanxia He (11797429), Ke Bao (9084820), Chunhong Hu, Xiujun Gao, Zhihan Jian (11797432), Chunhong Hu (105578), Xiujun Gao (3144987), Yuqing Zhao (50691), Jianbo Jian, Yuqing Zhao, Ke Bao, Zhihan Jian, Jianbo Jian (642712), Zhuanxia He   +13 more
core   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Biotin methyl ester enhances cargo release in RUSH system and enables rapid biotinylation with TurboID

Communications Biology
Biotin-streptavidin technology is a key tool in biotechnology. The engineering of streptavidin or streptavidin-like molecules and organic synthesis of biotin derivatives offer a variety of applications to meet the needs of researchers.
Taisei Uehara, Arata Takiguchi, Takuro Tojima, Akihiko Nakano, Shion Kagawa, Tatsuo Nehira, Takunori Satoh, Akiko K. Satoh   +7 more
doaj   +1 more source

MD1003 (high-dose biotin) for the treatment of progressive multiple sclerosis: A randomised, double-blind, placebo-controlled study

Multiple Sclerosis, 2016
Background: Treatment with MD1003 (high-dose biotin) showed promising results in progressive multiple sclerosis (MS) in a pilot open-label study. Objective: To confirm the efficacy and safety of MD1003 in progressive MS in a double-blind, placebo ...
A. Tourbah, C. Lebrun-Frénay, G. Edan, M. Clanet, C. Papeix, S. Vukusic, J. de Sèze, M. Debouverie, O. Gout, P. Clavelou, G. Defer, D. Laplaud, T. Moreau, P. Labauge, B. Brochet, F. Sedel, J. Pelletier   +16 more
semanticscholar   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source