Immunoproximity biotinylation reveals the axon initial segment proteome [PDF]
bioRxiv, 2023 The axon initial segment (AIS) is a specialized neuronal compartment required for action potential generation and neuronal polarity. However, understanding the mechanisms regulating AIS structure and function has been hindered by an incomplete knowledge ...
Wei Zhang +9 more
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Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation
Cells, 2020 BioID is a well-established method for identifying protein–protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15–18 h for robust biotinylation which may not be ideal ...
Danielle G. May +3 more
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Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments [PDF]
Nature Communications, 2023 Axon initial segment (AIS) cell surface proteins mediate key biological processes in neurons including action potential initiation and axo-axonic synapse formation. However, few AIS cell surface proteins have been identified.
Yuki Ogawa +12 more
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Off-the-shelf proximity biotinylation for interaction proteomics
Nature Communications, 2021 Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling ...
Irene Santos-Barriopedro +2 more
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AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions
eLife, 2020 Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism.
Kohki Kido +8 more
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Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome
Frontiers in Molecular Biosciences, 2022 In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms.
Ugo Dionne +2 more
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Super-resolution proximity labeling with enhanced direct identification of biotinylation sites
Communications BiologyPromiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein–protein interactions.
Sanghee Shin +6 more
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Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
Nature Methods, 2017 Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide ...
Namrata D. Udeshi +12 more
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Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes [PDF]
bioRxiv, 2020 Biotin-labeled molecular probes, comprising specific regions of the SARS-CoV-2 spike, would be helpful in the isolation and characterization of antibodies targeting this recently emerged pathogen.
Tongqing Zhou +37 more
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eLife, 2017 The cytosol-facing membranes of cellular organelles contain proteins that enable signal transduction, regulation of morphology and trafficking, protein import and export, and other specialized processes.
Victoria Hung +7 more
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