Results 1 to 10 of about 49,644 (261)

Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation [PDF]

Cells, 2020
BioID is a well-established method for identifying protein–protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15–18 h for robust biotinylation which may not be ideal ...
Danielle G. May, Kelsey L. Scott, Alexandre R. Campos, Kyle J. Roux   +3 more
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Analysis of Chemokine Receptor Trafficking by Site-Specific Biotinylation. [PDF]

PLoS ONE, 2016
Chemokine receptors undergo internalization and desensitization in response to ligand activation. Internalized receptors are either preferentially directed towards recycling pathways (e.g. CCR5) or sorted for proteasomal degradation (e.g. CXCR4). Here we
Marcel Liebick, Christian Schläger, Martin Oppermann   +2 more
doaj   +3 more sources

Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome [PDF]

Frontiers in Molecular Biosciences, 2022
In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms.
Ugo Dionne, Anne-Claude Gingras, Anne-Claude Gingras   +2 more
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AgrC biotinylation inhibits Staphylococcus aureus infection. [PDF]

PLoS ONE
Staphylococcus aureus (S. aureus) is a leading cause of nosocomial infections, particularly among antibiotic-resistant strains. S. aureus virulence is governed by the accessory gene regulator (Agr) quorum sensing (QS) system, which relies on AgrC, a two ...
Lijuan Qian, Yuxin He, Wenzhe Lian, Zhiyuan Ji, Ziming Tian, Chuyun Wang, Chen Cao, Tyler Shern, Teagan Stedman, Yujun Sun   +9 more
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Expression and Site-Specific Biotinylation of Human Cytosolic 5′-Nucleotidase 1A in Escherichia coli [PDF]

Methods and Protocols
Autoantibodies targeting cytosolic 5′-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren’s syndrome, and systemic lupus erythematosus.
Nataliya Slater, Anuradha Sooda, Frank L. Mastaglia, Sue Fletcher, Mark Watson, Merrilee Needham, Jerome D. Coudert   +6 more
doaj   +2 more sources

Tag Thy Neighbour: Nanometre-Scale Insights Into Kinetoplastid Parasites With Proximity Dependent Biotinylation

Frontiers in Cellular and Infection Microbiology, 2022
Proximity labelling is a powerful and rapidly developing technology for exploring the interaction space and molecular environment of a protein of interest at the nanometre scale.
Vincent Geoghegan, Jeremy C. Mottram, Nathaniel G. Jones   +2 more
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Coupling of Cell Surface Biotinylation and SILAC-Based Quantitative Proteomics Identified Myoferlin as a Potential Therapeutic Target for Nasopharyngeal Carcinoma Metastasis

Frontiers in Cell and Developmental Biology, 2021
Distant metastasis is a major cause of treatment failure in nasopharyngeal carcinoma (NPC) patients. Cell surface proteins represent attractive targets for cancer diagnosis or therapy. However, the cell surface proteins associated with NPC metastasis are
Maoyu Li, Maoyu Li, Fang Peng, Guoqiang Wang, Xujun Liang, Meiying Shao, Zhuchu Chen, Zhuchu Chen, Yongheng Chen, Yongheng Chen, Yongheng Chen   +10 more
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Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells

Frontiers in Cellular Neuroscience, 2022
Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells.
Rui Chen, Ningxia Zhang, Yubin Zhou, Ji Jing   +3 more
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Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83

Frontiers in Physiology, 2022
Juvenile hormone (JH) regulates insect development and reproduction through both intracellular and membrane signaling, and the two pathways might crosstalk with each other.
Yue Gao, Nan Chen, Xiangle Zhang, Emma Y. Li, Wei Luo, Jie Zhang, Wenqiang Zhang, Sheng Li, Sheng Li, Jian Wang, Suning Liu, Suning Liu   +11 more
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PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II

Cell Reports, 2022
Summary: Intrahepatic cholangiocarcinoma (ICC) contains abundant myofibroblasts derived from hepatic stellate cells (HSCs) through an activation process mediated by TGF-β.
Liankang Sun, Yuanguo Wang, Xianghu Wang, Amaia Navarro-Corcuera, Sumera Ilyas, Nidhi Jalan-Sakrikar, Can Gan, Xinyi Tu, Yu Shi, Kangsheng Tu, Qingguang Liu, Zhenkun Lou, Haidong Dong, Arlene H. Sharpe, Vijay H. Shah, Ningling Kang   +15 more
doaj   +1 more source