Results 1 to 10 of about 33,953 (257)
Biotinylation of Cell Surface Proteins [PDF]
Bio-Protocol, 2012 Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their ...
Guo Huang
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Revealing and mitigating the inhibitory effect of serotonin on HRP-mediated protein labelling [PDF]
Scientific ReportsProximity-dependent biotinylation coupled with mass spectrometry enables the characterization of subcellular proteomes. This technique has significantly advanced neuroscience by revealing sub-synaptic protein networks, such as the synaptic cleft and post-
Zora Chui-Kuen Chan +4 more
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In‐Depth Cell‐Type‐Specific Proteome Landscape of the Brain from Human Amyloid‐β Overexpression Mouse Model [PDF]
Advanced ScienceAmyloid‐β (Aβ) plays a crucial role in Alzheimer's disease pathogenesis. Understanding how Aβ overexpression alters the proteome of individual brain cell types is essential but challenging due to the nature of brain tissue, which contains intermingled ...
Taekyung Ryu +6 more
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AgrC biotinylation inhibits Staphylococcus aureus infection. [PDF]
PLoS ONEStaphylococcus aureus (S. aureus) is a leading cause of nosocomial infections, particularly among antibiotic-resistant strains. S. aureus virulence is governed by the accessory gene regulator (Agr) quorum sensing (QS) system, which relies on AgrC, a two ...
Lijuan Qian +9 more
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Expression and Site-Specific Biotinylation of Human Cytosolic 5′-Nucleotidase 1A in Escherichia coli [PDF]
Methods and ProtocolsAutoantibodies targeting cytosolic 5′-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren’s syndrome, and systemic lupus erythematosus.
Nataliya Slater +6 more
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Frontiers in Cellular and Infection Microbiology, 2022 Proximity labelling is a powerful and rapidly developing technology for exploring the interaction space and molecular environment of a protein of interest at the nanometre scale.
Vincent Geoghegan +2 more
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Frontiers in Cell and Developmental Biology, 2021 Distant metastasis is a major cause of treatment failure in nasopharyngeal carcinoma (NPC) patients. Cell surface proteins represent attractive targets for cancer diagnosis or therapy. However, the cell surface proteins associated with NPC metastasis are
Maoyu Li +10 more
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Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
Frontiers in Cellular Neuroscience, 2022 Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells.
Rui Chen +3 more
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Frontiers in Physiology, 2022 Juvenile hormone (JH) regulates insect development and reproduction through both intracellular and membrane signaling, and the two pathways might crosstalk with each other.
Yue Gao +11 more
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Adenovirus Fibers as Ultra-Stable Vehicles for Intracellular Nanoparticle and Protein Delivery
Biomolecules, 2022 Protein-based carriers are promising vehicles for the intracellular delivery of therapeutics. In this study, we designed and studied adenovirus protein fiber constructs with potential applications as carriers for the delivery of protein and nanoparticle ...
Chrysoula Kokotidou +6 more
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