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Biotinylation of Cell Surface Proteins [PDF]
Bio-Protocol, 2012 Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their ...
Guo Huang
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Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation
Cells, 2020 BioID is a well-established method for identifying protein–protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15–18 h for robust biotinylation which may not be ideal ...
Danielle G. May +3 more
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Off-the-shelf proximity biotinylation for interaction proteomics
Nature Communications, 2021 Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling ...
Irene Santos-Barriopedro +2 more
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Revealing and mitigating the inhibitory effect of serotonin on HRP-mediated protein labelling [PDF]
Scientific ReportsProximity-dependent biotinylation coupled with mass spectrometry enables the characterization of subcellular proteomes. This technique has significantly advanced neuroscience by revealing sub-synaptic protein networks, such as the synaptic cleft and post-
Zora Chui-Kuen Chan +4 more
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Super-resolution proximity labeling with enhanced direct identification of biotinylation sites
Communications BiologyPromiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein–protein interactions.
Sanghee Shin +6 more
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AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions
eLife, 2020 Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism.
Kohki Kido +8 more
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Nonenzymatic biotinylation of histone H2A [PDF]
Protein Science, 2009 AbstractHolocarboxylase synthetase (HCS, eukaryotic enzyme) and BirA (prokaryotic) are biotin protein ligases that catalyze the ATP‐dependent attachment of biotin to apocarboxylases via the reactive intermediate, bio‐5′‐AMP. In this study, we examined the in vitro mechanism of biotin attachment to histone H2A in the presence of HCS and BirA.
Shannon Healy +4 more
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High-Throughput Biotinylation of Proteins [PDF]
, 2008 One of the more useful tags for a protein in biochemical experiments is biotin, because of its femtomolar dissociation constant with streptavidin or avidin. Robust methodologies have been developed for other the in vivo addition of a single biotin to recombinant protein or the in vitro enzymatic or chemical addition of biotin to a protein.
Brian K. Kay +2 more
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In‐Depth Cell‐Type‐Specific Proteome Landscape of the Brain from Human Amyloid‐β Overexpression Mouse Model [PDF]
Advanced ScienceAmyloid‐β (Aβ) plays a crucial role in Alzheimer's disease pathogenesis. Understanding how Aβ overexpression alters the proteome of individual brain cell types is essential but challenging due to the nature of brain tissue, which contains intermingled ...
Taekyung Ryu +6 more
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AgrC biotinylation inhibits Staphylococcus aureus infection. [PDF]
PLoS ONEStaphylococcus aureus (S. aureus) is a leading cause of nosocomial infections, particularly among antibiotic-resistant strains. S. aureus virulence is governed by the accessory gene regulator (Agr) quorum sensing (QS) system, which relies on AgrC, a two ...
Lijuan Qian +9 more
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