Results 91 to 100 of about 33,953 (257)
Biotin attenuates heat shock factor 4b transcriptional activity by lysine 444 biotinylation
Biochemistry and Biophysics Reports, 2022 Genetic mutations in HSF4 cause congenital cataracts. HSF4 exhibits both positive and negative regulation on the transcription of heat shock and non-heat shock proteins during lens development, and its activity is regulated by posttranslational ...
Longjun Yan +18 more
doaj
Mapping protein–protein interactions by mass spectrometry
Mass Spectrometry Reviews, EarlyView.Abstract Protein–protein interactions (PPIs) are essential for numerous biological activities, including signal transduction, transcription control, and metabolism. They play a pivotal role in the organization and function of the proteome, and their perturbation is associated with various diseases, such as cancer, neurodegeneration, and infectious ...
Xiaonan Liu +4 more
wiley +1 more source
PLoS ONE, 2014 Dimerization of G protein-coupled receptors (GPCRs) represents a potential mechanism by which GPCR functions are regulated. Several resonance energy transfer (RET)-based methods have revealed GPCR homo- and heterodimerization.
Elisabeth Steel +2 more
doaj +1 more source
A toolbox for systematic discovery of stable and transient protein interactors in baker's yeast
Molecular Systems Biology, 2023 Identification of both stable and transient interactions is essential for understanding protein function and regulation. While assessing stable interactions is more straightforward, capturing transient ones is challenging.
Emma J Fenech +4 more
doaj +1 more source
PROTEOMICS, EarlyView.ABSTRACT Advances in methodologies and technologies over the past decade have led to an unprecedented depth of analysis of a cell's biomolecules, with entire genomes able to be sequenced in hours and up to 10,000 transcripts or ORF products (proteins) able to be quantified from a single cell.
Breyer Woodland +9 more
wiley +1 more source
PLoS ONE, 2019 The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10-14-10-16) has permitted this interaction to be used in a wide variety of experimental contexts.
Leslie M Stevens +4 more
doaj +1 more source
Proteomic Insight Into Alzheimer's Disease Pathogenesis Pathways
PROTEOMICS, EarlyView.ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism is still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing a complex network of dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation ...
Taekyung Ryu +3 more
wiley +1 more source
Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
Nature Methods, 2017 Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide ...
N. Udeshi +12 more
semanticscholar +1 more source
STAR Protocols, 2022 Summary: Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset
Serife Ayaz-Guner +10 more
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Frontiers in Plant Science, 2017 Proximity-dependent biotin identification (BioID), which detects physiologically relevant proteins based on the proximity-dependent biotinylation process, has been successfully used in different organisms.
Qiupeng Lin +7 more
doaj +1 more source