Results 91 to 100 of about 54,144 (311)

Responsive Visible‐Wavelength Metasurfaces from Porous Silicon Based on Fano Guided‐Mode Resonance

Advanced Optical Materials, EarlyView.
The article introduces mesoporous silicon metasurfaces (pSi‐MS) supporting Fano guided‐mode resonances in the visible regime. By combining top‐down lithographic patterning of 2D gratings with bottom‐up electrochemical porosification, the approach realizes a new class of responsive metasurfaces, enabling highly sensitive and portable platforms for small
Tomoshree Dash, Saddam Gafsi, Estevao Marques Dos Santos, Ivan Kravchenko, Judson D. Ryckman   +4 more
wiley   +1 more source

Dual-topology insertion of a dual-topology membrane protein. [PDF]

, 2015
Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear.
Bowie, James U, Woodall, Nicholas B, Yin, Ying   +2 more
core  

Direct measurement of transcription rates reveals multiple mechanisms for configuration of the Arabidopsis ambient temperature response [PDF]

, 2014
Background Sensing and responding to ambient temperature is important for controlling growth and development of many organisms, in part by regulating mRNA levels.
Costa, Maria J. (Maria João), Finkenstädt, Bärbel, Penfield, Steven, Rand, D. A. (David A.), Sidaway-Lee, Kate   +4 more
core   +1 more source

Diversity in functional organization of class I and class II biotin protein ligase. [PDF]

PLoS ONE, 2011
The cell envelope of Mycobacterium tuberculosis (M. tuberculosis) is composed of a variety of lipids including mycolic acids, sulpholipids, lipoarabinomannans, etc., which impart rigidity crucial for its survival and pathogenesis.
Sudha Purushothaman, Karthikeyan Annamalai, Anil K Tyagi, Avadhesha Surolia   +3 more
doaj   +1 more source

Type II Alveolar Epithelial Cells Promote Sepsis‐Induced Immunosuppression in Alveolar Macrophages via Exosomal lncRNA Rmrp Release

Advanced Science, EarlyView.
The S100A4/STAT3 axis promotes Rmrp transcription in AEC‐IIs and Rmrp expression in AEC‐II‐derived exosomes. Upon entry into AMs, Rmrp binds to and blocks the ubiquitination and degradation of ZFP36. Increased ZFP36 induced Pfkfb3 mRNA decay by binding to AREs in the 3′ UTR, curbing glycolysis and immune responses of AMs and aggravating SII and ...
Chengxi Liu, Weixia Xuan, Song Cao, Huayun Jia, Qian Wu, Xiaowu Tan, Qijie Wang, Xiaojun Li, Lisha Ding, Yaru Xiong, Meiyun Zhao, Longcheng Zheng, Yunzhu Xi, Jianhua Tan, Rong Li, Xulong Zhang, Wenjie Liu, Xu Wu   +17 more
wiley   +1 more source

Optimized In‐Solution and Gas‐Phase Chemistry Enables High‐Efficiency Interactome Mapping by DSBSO‐Based Cross‐Linking Mass Spectrometry

Angewandte Chemie, EarlyView.
We developed a rapid, high‐throughput XL–MS workflow using the enrichable azide‐A‐DSBSO cross‐linker and StageTip‐based SCX fractionation. It drastically reduces preparation time and enhances sensitivity, providing a scalable platform for high‐throughput profiling of protein interaction networks.
Pin‐Lian Jiang, Ying Zhu, Jiaxin Cai, Cong Wang, Mei Wu, Ke Pu, Fan Liu   +6 more
wiley   +2 more sources

A General Method to Screen Nanobodies for Cytochrome P450 Enzymes from a Yeast Surface Display Library

Biomedicines
The availability of yeast surface display nanobody (Nb) libraries offers a convenient way to acquire antigen-specific nanobodies that may be useful for protein structure–function studies and/or therapeutic applications, complementary to the conventional ...
Yudong Sun, Cristian Martinez-Ramos, Eugene Chen, Yoichi Osawa, Haoming Zhang   +4 more
doaj   +1 more source

Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking

BioTechniques, 2011
We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which ...
Faraz Ahmad, Sarah Kate Coleman, Kai Kaila, Peter Blaesse   +3 more
doaj   +1 more source

Analysis of Chemokine Receptor Trafficking by Site-Specific Biotinylation. [PDF]

PLoS ONE, 2016
Chemokine receptors undergo internalization and desensitization in response to ligand activation. Internalized receptors are either preferentially directed towards recycling pathways (e.g. CCR5) or sorted for proteasomal degradation (e.g. CXCR4). Here we
Marcel Liebick, Christian Schläger, Martin Oppermann   +2 more
doaj   +1 more source

Inhibiting Liver‐Derived C3 Protein Rescues Anesthesia/Surgery‐Induced Cognitive Impairment, Synaptic Disorders, and Microglial Phagocytosis

Advanced Science, EarlyView.
This study provides evidence that anesthesia/surgery can induce the BBB breakdown and promote the release of hepatogenous C3 protein into the blood. This surgical dual factors ultimately drove C3 to cross the damaged BBB and selectively colocalize with C3aR in the hippocampus, which results in structural and functional injury of synapse, C3aR‐mediated ...
Qianqian Wu, Peilin Cong, Zhouxiang Li, Yuxin Zhang, Huanghui Wu, Qian Zhang, Yawei Li, Li Tian, Qingyuan Miao, Yinggang Zheng, Hui Zhang, Qian Chen, Enduo Feng, Xinyang Li, Zheping Chen, Dong‐Xin Wang, Xinwei Huang, Lize Xiong   +17 more
wiley   +1 more source