Results 101 to 110 of about 33,953 (257)

Structural and Functional Characterization of the 28 kDa Structured Core of BmSA1, the Major Surface Antigen of Babesia Microti

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Babesiosis is a tick‐borne disease that poses a significant threat to animal health worldwide. In addition, climate change and the risk of human‐to‐human transmission through blood transfusion have made babesiosis an emerging disease in humans.
Assia Mouhand, Joana Pissarra, Philippe Barthe, Christian Roumestand, Stéphane Delbecq   +4 more
wiley   +1 more source

Secretome Profiling in Axon Regeneration: Emerging Techniques and Therapeutic Translation

Sensory Neuroscience, EarlyView.
ABSTRACT The secretome refers to the in vivo ensemble of molecular entities that are found in interstitial spaces. It is then the mere secreted products of the cells, as all biomolecules in the interstitial cellular spaces also undergo modifications. The fractionated secretome may hold potential as a cell‐free therapy option due to its role in tissue ...
Maria D. Cabrera, Nicole Ramones, Isabella Moceri, Sanjoy K. Bhattacharya   +3 more
wiley   +1 more source

DNA Hanger: Surface‐Minimized Single‐Molecule Immunoassay Platform

Small, EarlyView.
The DNA Hanger platform suspends long DNA molecules biotinylated along their length away from the surface, effectively minimizing nonspecific protein binding. The assay achieves high sensitivity and specificity in single‐molecule FLISA down to subpicomolar levels.
Jincheol Seol, Byungju Kim, Eui‐Sang Yu, Cherlhyun Jeong, Jong‐Bong Lee   +4 more
wiley   +1 more source

Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules

Biophysical Journal, 1991
Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography.
Elizabeth W. Kubalek, R. Blankenburg, Roger D. Kornberg, H. Ringsdorf, Seth A. Darst, Hans O. Ribi, P.H. Meller, M. Ahlers   +7 more
openaire   +3 more sources

Identification of MEF2A, MEF2C, and MEF2D interactomes in basal and Fsk‐stimulated mouse MA‐10 Leydig cells

Andrology, EarlyView.
Abstract Background Myocyte enhancer factor 2 transcription factors regulate essential transcriptional programs in various cell types. The activity of myocyte enhancer factor 2 factors is modulated through interactions with cofactors, chromatin remodelers, and other regulatory proteins, which are dependent on cell context and physiological state.
Karine de Mattos, Marie‐Pier Scott‐Boyer, Arnaud Droit, Robert S. Viger, Jacques J. Tremblay   +4 more
wiley   +1 more source

Biotinylation by antibody recognition - A method for proximity labeling

Nature Methods, 2017
The high-throughput detection of organelle composition and proteomic mapping of protein environment directly from primary tissue as well as the identification of interactors of insoluble proteins that form higher-order structures have remained challenges
D. Bar, Kathleen Atkatsh, Urraca Tavarez, M. Erdos, Y. Gruenbaum, F. Collins   +5 more
semanticscholar   +1 more source

Elucidating the Role and Mechanism of Alpha‐Enolase in Senescent Amelioration via Metabolic Reprogramming

Cell Proliferation, EarlyView.
KB2764 interacted with alpha‐enolase (ENO1) and pyruvate kinase M (PKM), ultimately leading to PKM phosphorylation of ENO1. KB2764 consequently increased mitochondrial ATP production and decreased glycolysis dependence. Furthermore, the action of KB2764 extends its application to extend the lifespan of Caenorhabditis elegans.
Yun Haeng Lee, Hyunwoong Lim, Gyungmin Kim, Geonhee Jang, Myeong Uk Kuk, Ji Ho Park, Jee hee Yoon, Yoo Jin Lee, Duyeol Kim, Byeonghyeon So, Minseon Kim, Hyung Wook Kwon, Youngjoo Byun, Joon Tae Park   +13 more
wiley   +1 more source

Author Correction: A proximity-dependent biotinylation map of a human cell

Nature, 2022
Christopher D. Go, J. Knight, Archita Rajasekharan, B. Rathod, G. Hesketh, K. Abe, J. Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y Zhu, Evelyn Popiel, Jean-Philippe Lambert, É. Coyaud, Sally W T Cheung, D. Rajendran, Cassandra J. Wong, H. Antonicka, L. Pelletier, Alexander F. Palazzo, E. Shoubridge, B. Raught, A. Gingras   +21 more
semanticscholar   +1 more source

LL‐37 Inhibits TMPRSS2‐Mediated S2' Site Cleavage and SARS‐CoV‐2 Infection but Not Omicron Variants

Cell Proliferation, EarlyView.
Cathelicidin LL‐37 inhibits SARS‐CoV‐2 by blocking the catalytic activity of TMPRSS2. The N764K mutation in the spike S2 subunit of Omicron variants confers resistance to LL‐37‐induced inhibition of TMPRSS2‐mediated S2' priming. ABSTRACT Continual evolution of SARS‐CoV‐2 spike drives the emergence of Omicron variants that show increased spreading and ...
Zhenfei Bi, Wenyan Ren, Hao Zeng, Yuanyuan Zhou, Jian Liu, Zimin Chen, Xindan Zhang, Xuemei He, Guangwen Lu, Yuquan Wei, Xiawei Wei   +10 more
wiley   +1 more source

A guide to heat shock factors as multifunctional transcriptional regulators

The FEBS Journal, EarlyView.
The heat shock factors (HSFs) are evolutionarily conserved transcription factors best known as regulators of molecular chaperone genes in response to heat shock and other protein‐damaging stresses. Vertebrate HSFs, HSF1‐5, HSFX, and HSFY, are implicated in various physiological and pathological processes, including organismal development and cancer ...
Hendrik S. E. Hästbacka, Alejandro J. Da Silva, Lea Sistonen, Eva Henriksson   +3 more
wiley   +1 more source