Results 101 to 110 of about 49,644 (261)

Screening of Proximal and Interacting Proteins in Rice Protoplasts by Proximity-Dependent Biotinylation

Frontiers in Plant Science, 2017
Proximity-dependent biotin identification (BioID), which detects physiologically relevant proteins based on the proximity-dependent biotinylation process, has been successfully used in different organisms.
Qiupeng Lin, Zejiao Zhou, Wanbin Luo, Maichun Fang, Maichun Fang, Meiru Li, Meiru Li, Hongqing Li   +7 more
doaj   +1 more source

High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme [PDF]

, 2020
Prince Kumar Lat, Kun Liu, Dev N Kumar, Kenneth Kin Lam Wong, Esther M. Verheyen, Dipankar Sen   +5 more
openalex   +1 more source

Nanopore Trap for Label‐Free Fingerprinting of Surface‐modified Single Nanoparticles

Small Methods, EarlyView.
A solid‐state nanopore trapping‐based methodology combines “trap‐release” measurement protocol with multiple features extracted from ionic current of trapping events to generate unique electrical fingerprints of nanoparticles with distinct surface functional layers.
Nianduo Cai, Tzu‐Heng Chen, Yunfei Teng, Akhil Sai Naidu, Aleksandra Radenovic   +4 more
wiley   +1 more source

Signal-On Detection of Caspase-3 with Methylene Blue-Loaded Metal-Organic Frameworks as Signal Reporters

Molecules
In this work, we report on an electrochemical method for the signal-on detection of caspase-3 and the evaluation of apoptosis based on the biotinylation reaction and the signal amplification of methylene blue (MB)-loaded metal–organic frameworks (MOFs ...
Yaliang Huang, Jiaqiang Wang, Yirui Xu, Jiwen Zhang, Ning Xia   +4 more
doaj   +1 more source

Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides

Journal of Analytical Methods in Chemistry, 2016
Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5 ...
Chun Wu
doaj   +1 more source

Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches. [PDF]

, 2018
Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes.
Bennett, Eric J, Ghosh, Arit, Leonard, Marilyn, Liao, Jeffrey, Yang, Bing, Zuzow, Nathan   +5 more
core   +2 more sources

Recent Advances in Bioconjugation of Aromatic Amino Acid Residues by a Reactivity‐Guided Approach

The Chemical Record, EarlyView.
This review highlights recent advances in the bioconjugation of aromatic amino acids residues, focusing on strategies that leverage their inherent chemical reactivity to enable precise and versatile modifications of biomacromolecules, illustrating relevant applications.
Bruno M. da S. Santos, Lívia C. R. M. da Frota, Thais G. Silva, Fernanda G. Finelli   +3 more
wiley   +1 more source

The Importance of Biotinylation for the Suitability of Cationic and Neutral Fourth-Generation Polyamidoamine Dendrimers as Targeted Drug Carriers in the Therapy of Glioma and Liver Cancer

Molecules
In this study, we hypothesized that biotinylated and/or glycidol-flanked fourth-generation polyamidoamine (PAMAM G4) dendrimers could be a tool for efficient drug transport into glioma and liver cancer cells.
Łukasz Uram, Magdalena Twardowska, Żaneta Szymaszek, Maria Misiorek, Andrzej Łyskowski, Zuzanna Setkowicz, Zuzanna Rauk, Stanisław Wołowiec   +7 more
doaj   +1 more source

Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation.

PLoS ONE, 2019
The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10-14-10-16) has permitted this interaction to be used in a wide variety of experimental contexts.
Leslie M Stevens, Yuan Zhang, Yuri Volnov, Geng Chen, David S Stein   +4 more
doaj   +1 more source

Genetic Encoding of a Trifunctional Photo‐Cross‐Linker with a Cleavable Alkyl Ester Moiety [PDF]

Chembiochem
A novel noncanonical amino acid, DiZAAsu, possessing photo‐cross‐linking, biotinylation, and alkaline‐cleavable functionalities, is genetically encoded by an engineered pyrrolyl‐tRNA synthetase mutant. The alkyl ester moiety expands the design space of cleavable photo‐cross‐linkable amino acids.
Takayama M, Tsubota T, Yamaguchi T, Chiba K, Yoshida T, Hari Y, Tian Y, Takaya D, Mori A, Tsukamoto T, Ishimoto K, Ago Y, Okada Y, Sakamoto K, Doi T, Fukuzawa K, Obika S, Nakagawa S, Hino N.   +18 more
europepmc   +2 more sources