Results 111 to 120 of about 33,953 (257)

BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation.

Journal of Proteome Research, 2017
Biotin-based labeling strategies are widely employed to study protein-protein interactions, subcellular proteomes and post-translational modifications, as well as, used in drug discovery.
Dae In Kim, J. Cutler, C. Na, S. Reckel, S. Renuse, Anil K. Madugundu, Raiha Tahir, Hana L. Goldschmidt, K. Reddy, R. Huganir, Xinyan Wu, N. Zachara, O. Hantschel, A. Pandey   +13 more
semanticscholar   +1 more source

A guide to characterizing the dynamic mitochondria–endoplasmic reticulum contact sites

The FEBS Journal, EarlyView.
The dynamism, heterogeneity, and functional complexity of mitochondria–endoplasmic reticulum contacts (MERCs) require combined methodological approaches to offset limitations of individual techniques. Here, we review available tools and methodologies for MERC research, outline key technical challenges, and discuss how recent advances deliver ...
Antigoni Diokmetzidou, Luca Scorrano
wiley   +1 more source

Targeting protein biotinylation enhances tuberculosis chemotherapy

Science Translational Medicine, 2018
Inhibitors of biotin protein ligase and protein biotinylation in Mycobacterium tuberculosis act synergistically with the drug rifampicin, potentially shortening tuberculosis treatment.
Divya Tiwari, S. W. Park, Maram M. Essawy, Surendra Dawadi, Alan Mason, Madhumitha Nandakumar, M. Zimmerman, Marizel Mina, H. Ho, Curtis A. Engelhart, T. Ioerger, J. Sacchettini, K. Rhee, S. Ehrt, C. Aldrich, V. Dartois, D. Schnappinger   +16 more
semanticscholar   +1 more source

Modulating the activity of the APC/C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits

Plant Biotechnology Journal, EarlyView.
Summary The Anaphase‐Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that plays a crucial role in ubiquitin‐dependent proteolysis of key cell cycle regulators, which is completed by the 26S proteasome. Previously, SAMBA, a plant‐specific regulator of the APC/C, was identified in Arabidopsis as a critical factor controlling organ size ...
Perla Novais de Oliveira, Leonardo Perez de Souza, Pedro Boscariol Ferreira, Jean Phillippe Mauxion, Luis Felipe Correa da Silva, Ana Isabela Chang, Marina de Lyra Soriano Saleme, Feng Zhu, Sara Selma García, Herman De Beukelaer, Marina C. M. Martins, Laise Rosado‐Souza, Lilian Ellen Pino, Norbert Bollier, Lázaro Eustáquio Pereira Peres, Klaas Vandepoele, Alain Goossens, Nathalie Gonzalez, Alisdair R. Fernie, Nubia Barbosa Eloy   +19 more
wiley   +1 more source

Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System

Bio-Protocol, 2018
Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo biotinylation system
Weronika Sura, Piotr Ziolkowski
doaj   +1 more source

Cell-surface biotinylation of GLUT4 using bis-mannose photolabels [PDF]

, 1998
Françoise Koumanov, Jing Yang, E. Alison JONES, Yasumaru Hatanaka, Geoffrey D. Holman   +4 more
openalex   +1 more source

Kinase-catalyzed biotinylation of DNA

Bioorganic & Medicinal Chemistry, 2018
Prior work documented use of γ-phosphate modified ATP analogs to label DNA using T4 polynucleotide kinases (T4PNK), although applications have been limited. To fully characterize kinase-catalyzed labeling of nucleic acids, we explored use of ATP-biotin as a cosubstrate with T4PNK. T4PNK accepted ATP-biotin to 5'-label single stranded DNA.
Mary Kay H. Pflum, Thilani M. Anthony
openaire   +4 more sources

A Universal Approach to Anchoring Chromophores onto Magnetic Scaffold for Achieving Easily Recyclable Heterogeneous Photocatalytic Systems

Advanced Science, Volume 12, Issue 26, July 10, 2025.
The practical application of chromophores such as porphyrins, coumarins, anthraquinones, and pyrene derivatives in photocatalysis is severely limited by catalyst stability and reusability. In this work, a simple and universal strategy is presented for preparing highly efficient and recyclable heterogeneous photocatalysts for the oxidative coupling of ...
Xuan Zhan, Yikun Wang, Chenyang Sun, Yuchen Fang, Lihua Huang, Zakir Ullah, Qiang Chen, Xuejing Wang, Zheng Xing, Gangfeng Ouyang   +9 more
wiley   +1 more source

In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis

mBio, 2016
Toxoplasma gondii is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into the nascent
S. Nadipuram, Elliot W. Kim, A. Vashisht, Andrew H. Lin, Hannah N. Bell, I. Coppens, J. Wohlschlegel, P. Bradley   +7 more
semanticscholar   +1 more source

Production of in vivo-biotinylated rotavirus particles

Journal of General Virology, 2012
Although inserting exogenous viral genome segments into rotavirus particles remains a hard challenge, this study describes the in vivo incorporation of a recombinant viral capsid protein (VP6) into newly assembled rotavirus particles. In vivo biotinylation technology was exploited to biotinylate a recombinant VP6 protein fused to a 15 aa biotin ...
De Lorenzo, G., Eichwald, C., Schraner, E. M., Nicolin, V., Roberta, Bortul, Mano, M., Burrone, O. R., Arnoldi, F.   +7 more
openaire   +5 more sources