Angewandte Chemie, Volume 138, Issue 4, 22 January 2026.We developed a rapid, high‐throughput XL–MS workflow using the enrichable azide‐A‐DSBSO cross‐linker and StageTip‐based SCX fractionation. It drastically reduces preparation time and enhances sensitivity, providing a scalable platform for high‐throughput profiling of protein interaction networks.
Pin‐Lian Jiang +6 more
wiley +2 more sources
Differences in Src phosphorylation of PSD‐93 and PSD‐95 drive differences in scaffolding activity
Protein Science, Volume 35, Issue 2, February 2026.Abstract Scaffold proteins contain multiple binding modules that allow for co‐localization of proteins that lack a direct interaction. Evolution resulted in different combinations of binding modules that rewired existing signal transduction pathways.
Frank A. Mindlin +3 more
wiley +1 more source
Glycolytic enzymes - novel carbohydrate binding proteins for glycoprotein analysis [PDF]
, 2011 •The cloning, expression, purification and characterisation of recombinant prokaryotic glycolytic enzymes •The mutagenesis of prokaryotic glycolytic enzymes to generate novel recombinant carbohydrate binding proteins •The characterisation of the ...
Clarke, Paul A. +3 more
core
Protein Science, Volume 35, Issue 2, February 2026.Abstract Detergents provide essential membrane‐mimetic environments for studying G protein‐coupled receptors (GPCRs), but their molecular impact on receptor energetics remains incompletely understood. We combined ligand binding, thermostability measurements, and atomistic molecular dynamics to dissect detergent‐ versus ligand‐driven stabilization in a ...
James B. Bower +6 more
wiley +1 more source
Super-resolution proximity labeling with enhanced direct identification of biotinylation sites
Communications BiologyPromiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein–protein interactions.
Sanghee Shin +6 more
doaj +1 more source
Bacterial Expression of Gene‐Encoding DNA Origami Structures
Small Structures, Volume 7, Issue 2, February 2026.Bacterial targeting via programmable DNA origami, varying in rigidity and compactness is unexplored. In transformation assays, origami reduces transformation efficiency compared to single‐stranded scaffolds. Structural features and surface modifications, however, improve transformation outcomes.
Verena Dobretzberger +7 more
wiley +1 more source
From aptamer-based biomarker discovery to diagnostic and clinical applications: an aptamer-based, streamlined multiplex proteomic assay [PDF]
, 2010 Recently, we reported an aptamer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life ...
Christopher Bock +10 more
core +1 more source
Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation [PDF]
, 2019 Leslie M. Stevens +4 more
openalex +1 more source
A toolbox for efficient proximity-dependent biotinylation in zebrafish embryos [PDF]
, 2021 Shimon M. Rosenthal +6 more
openalex +1 more source
Cytokine Profiles of Stimulated Blood Lymphocytes in Asthmatic and Healthy Adolescents Cross the School Year [PDF]
, 1997 T cell cytokines play an important role in mediating airway inflammation in asthma. The predominance of a Th2 cytokine profile, particularly interleukin (IL)-4 and IL-5, is associated with the pathogenesis and course of asthma.
Busse, William W. +6 more
core +1 more source