Results 121 to 130 of about 54,144 (311)

The Fusion Activity of HIV-1 gp41 Depends on Interhelical Interactions [PDF]

, 2005
Infection by human immunodeficiency virus type I requires the fusogenic activity of gp41, the transmembrane subunit of the viral envelope protein. Crystallographic studies have revealed that fusion-active gp41 is a "trimer-of-hairpins" in which three ...
Chan, David C., Suntoke, Tara R.
core   +1 more source

Biotinylated Phosphoproteins from Kinase‐Catalyzed Biotinylation are Stable to Phosphatases: Implications for Phosphoproteomics [PDF]

ChemBioChem, 2013
AbstractKinase‐catalyzed protein phosphorylation is involved in a wide variety of cellular events. Development of methods to monitor phosphorylation is critical to understand cell biology. Our lab recently discovered kinase‐catalyzed biotinylation, where ATP‐biotin is utilized by kinases to label phosphopeptides or phosphoproteins with a biotin tag. To
Chamara, Senevirathne, Mary Kay H, Pflum
openaire   +2 more sources

Targeted 2‐Deoxy‐D‐Ribose Delivery by Biomimetic Nanoplatform Activates EGFR for Accelerated Heart Valve Endothelialization

Advanced Science, EarlyView.
This study develops a biomimetic nanoplatform using erythrocyte membrane‐camouflaged, CD144 antibody‐functionalized nanoparticles to deliver 2‐deoxy‐D‐ribose (2dDR). This system promotes heart valve endothelialization by enhancing endothelial cell migration and proliferation via EGFR activation, while improving hemocompatibility.
Xiang Qiu, Gaofeng Li, Wenyi Wan, Jinsheng Li, Ge Yan, Shijie Wang, Xiuqi Hu, Zongqi Han, Yazheng Shan, Ying Zhou, Nianguo Dong, Weihua Qiao   +11 more
wiley   +1 more source

Multiplex detection of homo- and heterodimerization of g protein-coupled receptors by proximity biotinylation.

PLoS ONE, 2014
Dimerization of G protein-coupled receptors (GPCRs) represents a potential mechanism by which GPCR functions are regulated. Several resonance energy transfer (RET)-based methods have revealed GPCR homo- and heterodimerization.
Elisabeth Steel, Victoria L Murray, Allen P Liu   +2 more
doaj   +1 more source

Minihepcidins are rationally designed small peptides that mimic hepcidin activity in mice and may be useful for the treatment of iron overload [PDF]

, 2011
Iron overload is the hallmark of hereditary hemochromatosis and a complication of iron-loading anemias such as β-thalassemia. Treatment can be burdensome and have significant side effects, and new therapeutic options are needed.
Alan Waring, Bo Qiao, Elizabeta Nemeth, Emilio Ramos, Fujita, Gloria C. Preza, Gualdi, Kearney, Lyskov, Michael A. Peralta, Nemeth, Piotr Ruchala, Porter, Rogelio Pinon, Sham, Shantanu Sharma, Tomas Ganz   +16 more
core   +2 more sources

Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides

Journal of Analytical Methods in Chemistry, 2016
Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5 ...
Chun Wu
doaj   +1 more source

Screening of Proximal and Interacting Proteins in Rice Protoplasts by Proximity-Dependent Biotinylation

Frontiers in Plant Science, 2017
Proximity-dependent biotin identification (BioID), which detects physiologically relevant proteins based on the proximity-dependent biotinylation process, has been successfully used in different organisms.
Qiupeng Lin, Zejiao Zhou, Wanbin Luo, Maichun Fang, Maichun Fang, Meiru Li, Meiru Li, Hongqing Li   +7 more
doaj   +1 more source

Signal-On Detection of Caspase-3 with Methylene Blue-Loaded Metal-Organic Frameworks as Signal Reporters

Molecules
In this work, we report on an electrochemical method for the signal-on detection of caspase-3 and the evaluation of apoptosis based on the biotinylation reaction and the signal amplification of methylene blue (MB)-loaded metal–organic frameworks (MOFs ...
Yaliang Huang, Jiaqiang Wang, Yirui Xu, Jiwen Zhang, Ning Xia   +4 more
doaj   +1 more source

The Importance of Biotinylation for the Suitability of Cationic and Neutral Fourth-Generation Polyamidoamine Dendrimers as Targeted Drug Carriers in the Therapy of Glioma and Liver Cancer

Molecules
In this study, we hypothesized that biotinylated and/or glycidol-flanked fourth-generation polyamidoamine (PAMAM G4) dendrimers could be a tool for efficient drug transport into glioma and liver cancer cells.
Łukasz Uram, Magdalena Twardowska, Żaneta Szymaszek, Maria Misiorek, Andrzej Łyskowski, Zuzanna Setkowicz, Zuzanna Rauk, Stanisław Wołowiec   +7 more
doaj   +1 more source

Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation.

PLoS ONE, 2019
The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10-14-10-16) has permitted this interaction to be used in a wide variety of experimental contexts.
Leslie M Stevens, Yuan Zhang, Yuri Volnov, Geng Chen, David S Stein   +4 more
doaj   +1 more source