Results 131 to 140 of about 54,144 (311)
Kinase-catalyzed biotinylation of DNA
Bioorganic & Medicinal Chemistry, 2018 Prior work documented use of γ-phosphate modified ATP analogs to label DNA using T4 polynucleotide kinases (T4PNK), although applications have been limited. To fully characterize kinase-catalyzed labeling of nucleic acids, we explored use of ATP-biotin as a cosubstrate with T4PNK. T4PNK accepted ATP-biotin to 5'-label single stranded DNA.
Thilani M, Anthony, Mary Kay H, Pflum
openaire +3 more sources
Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches. [PDF]
, 2018 Previous genetic and biochemical studies from Saccharomyces cerevisiae have identified a critical ribosome-associated quality control complex (RQC) that facilitates resolution of stalled ribosomal complexes.
Bennett, Eric J +5 more
core +2 more sources
Data‐Independent Acquisition Mass Spectrometry in Tumor Classification and Cancer Biomarker Research
Mass Spectrometry Reviews, EarlyView.Abstract Cancer treatment is far from optimal also because current classification systems do not reflect the complex molecular status of the tumor and its phenotype in sufficient detail. To construct molecular tumor classifiers, omics tools provide complex molecular data reflecting many aspects from genotype to phenotype.
Jan Simonik +3 more
wiley +1 more source
Advancing Extracellular Vesicle Research: A Review of Systems Biology and Multiomics Perspectives
PROTEOMICS, EarlyView.ABSTRACT Extracellular vesicles (EVs) are membrane‐bound vesicles secreted by various cell types into the extracellular space and play a role in intercellular communication. Their molecular cargo varies depending on the cell of origin and its functional state.
Gloria Kemunto +2 more
wiley +1 more source
Oxygen/Glucose deprivation induces a reduction in synaptic AMPA receptors on hippocampal CA3 neurons mediated by mGluR1 and adenosine A3 receptors [PDF]
, 2011 Hippocampal CA1 pyramidal neurons are highly sensitive to ischemic damage, whereas neighboring CA3 pyramidal neurons are less susceptible. It is proposed that switching of AMPA receptor (AMPAR) subunits on CA1 neurons during an in vitro model of ischemia,
Cimarosti, Helena +5 more
core +1 more source
Nanopore Trap for Label‐Free Fingerprinting of Surface‐modified Single Nanoparticles
Small Methods, EarlyView.A solid‐state nanopore trapping‐based methodology combines “trap‐release” measurement protocol with multiple features extracted from ionic current of trapping events to generate unique electrical fingerprints of nanoparticles with distinct surface functional layers.
Nianduo Cai +4 more
wiley +1 more source
Scientific Reports, 2019 The complement of cell surface proteins, collectively referred to as the surfaceome, is a useful indicator of normal differentiation processes, and the development of pathologies such as osteoarthritis (OA). We employed biochemical and proteomic tools to
Csaba Matta +7 more
semanticscholar +1 more source
Recent Advances in Bioconjugation of Aromatic Amino Acid Residues by a Reactivity‐Guided Approach
The Chemical Record, EarlyView.This review highlights recent advances in the bioconjugation of aromatic amino acids residues, focusing on strategies that leverage their inherent chemical reactivity to enable precise and versatile modifications of biomacromolecules, illustrating relevant applications.
Bruno M. da S. Santos +3 more
wiley +1 more source
Bio-Protocol, 2018 Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo biotinylation system
Weronika Sura, Piotr Ziolkowski
doaj +1 more source
Phenoxy Radical Reactivity of Nucleic Acids: Practical Implications for Biotinylation [PDF]
, 2020 Brandon Wilbanks +4 more
openalex +1 more source