Results 141 to 150 of about 54,144 (311)

Author Correction: A proximity-dependent biotinylation map of a human cell

Nature, 2022
Christopher D. Go, J. Knight, Archita Rajasekharan, B. Rathod, G. Hesketh, K. Abe, J. Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y Zhu, Evelyn Popiel, Jean-Philippe Lambert, É. Coyaud, Sally W T Cheung, D. Rajendran, Cassandra J. Wong, H. Antonicka, L. Pelletier, Alexander F. Palazzo, E. Shoubridge, B. Raught, A. Gingras   +21 more
semanticscholar   +1 more source

Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins

Science Signaling, 2019
The tumor suppressor Merlin interacts with cell junction–associated proteins and with the proapoptotic protein ASPP2. The Merlin interactome identifies cell junction proteins Merlin (also called NF2) is a tumor suppressor that associates with lipid rafts
R. Hennigan, Jonathan S. Fletcher, S. Guard, N. Ratner   +3 more
semanticscholar   +1 more source

18S and 25S Exonuclease Resistant Ribosomal RNA Molecules Are Produced by 5′‐End Modification During TOR Inhibition.

Yeast, EarlyView.
Saccharomyces cerevisiae produces exonuclease‐resistant rRNA molecules upon TOR inhibition. This phenomenon occurs when cells enter the diauxic phase, upon addition of rapamycin to the culture, or when TOR activity is reduced through deletion of the TOR1 gene.
Miguel A. Rocha, Gowda Bhavani, Jacob Fleischmann   +2 more
wiley   +1 more source

Reversible Sandwich‐Based Particle Nanoswitch for Continuous Protein Monitoring at Picomolar Concentrations with Automated Calibration

Angewandte Chemie, Volume 137, Issue 51, December 15, 2025.
A reversible particle nanoswitch sensor enables continuous protein monitoring by forming transient sandwich complexes in the presence of analyte molecules. Site‐specific conjugations ensure optimal antibody fragment orientation. Demonstrated for lactoferrin, the sensor achieves picomolar sensitivity with ∼12 min time delay and continuous measurements ...
Claire M. S. Michielsen, Junhong Yan, Max H. Bergkamp, Yu‐Ting Lin, Stijn R. R. Haenen, Rafiq M. Lubken, Alexander Gräwe, Anna Swietlikowska, Arthur M. de Jong, Menno W. J. Prins   +9 more
wiley   +2 more sources

Biotinylation by antibody recognition - A method for proximity labeling

Nature Methods, 2017
The high-throughput detection of organelle composition and proteomic mapping of protein environment directly from primary tissue as well as the identification of interactors of insoluble proteins that form higher-order structures have remained challenges
D. Bar, Kathleen Atkatsh, Urraca Tavarez, M. Erdos, Y. Gruenbaum, F. Collins   +5 more
semanticscholar   +1 more source

Identification of MEF2A, MEF2C, and MEF2D interactomes in basal and Fsk‐stimulated mouse MA‐10 Leydig cells

Andrology, EarlyView.
Abstract Background Myocyte enhancer factor 2 transcription factors regulate essential transcriptional programs in various cell types. The activity of myocyte enhancer factor 2 factors is modulated through interactions with cofactors, chromatin remodelers, and other regulatory proteins, which are dependent on cell context and physiological state.
Karine de Mattos, Marie‐Pier Scott‐Boyer, Arnaud Droit, Robert S. Viger, Jacques J. Tremblay   +4 more
wiley   +1 more source

Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules

Biophysical Journal, 1991
Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography.
Darst, S.A., Ahlers, M., Meller, P.H., Kubalek, E.W., Blankenburg, R., Ribi, H.O., Ringsdorf, H., Kornberg, R.D.   +7 more
openaire   +2 more sources

The importin‐alpha superfamily engages in ethylene signaling by shuttling ETHYLENE INSENSITIVE 2 from the endoplasmic reticulum to the nucleus

The FEBS Journal, EarlyView.
The plant hormone ethylene regulates plant growth, ripening, senescence, and stress responses. The hormonal signal transmission, from receptors at the ER membrane to the transcriptional regulators in the nucleus, is still not completely understood.
Fabian Wynen, Jan Eric Maika, Raphael Josef Eberle, Nina Jahnke, Marcel Wiermer, Laura Hartmann, Rüdiger Simon, Georg Groth   +7 more
wiley   +1 more source

From aptamer-based biomarker discovery to diagnostic and clinical applications: an aptamer-based, streamlined multiplex proteomic assay [PDF]

, 2010
Recently, we reported an aptamer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life ...
Christopher Bock, Dom Zichi, Evaldas Katilius, Glenn M. Sanders, Jonathon D. Vaught, Larry Gold, Nancy Kim, Nicholas A. Saccomano, Sheri K. Wilcox, Stephan Kraemer, Tracy R. Keeney   +10 more
core   +1 more source

β-Actin mRNA interactome mapping by proximity biotinylation

Proceedings of the National Academy of Sciences of the United States of America, 2018
Significance Transport of specific mRNAs to defined sites in the cytoplasm allows local protein production and contributes to cell polarity, embryogenesis, and neuronal function.
Joyita Mukherjee, O. Hermesh, C. Eliscovich, N. Nalpas, M. Franz‐Wachtel, B. Macek, R. Jansen   +6 more
semanticscholar   +1 more source