Revealing and mitigating the inhibitory effect of serotonin on HRP-mediated protein labelling [PDF]
Scientific ReportsProximity-dependent biotinylation coupled with mass spectrometry enables the characterization of subcellular proteomes. This technique has significantly advanced neuroscience by revealing sub-synaptic protein networks, such as the synaptic cleft and post-
Zora Chui-Kuen Chan +4 more
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A toolbox for efficient proximity-dependent biotinylation in zebrafish embryos [PDF]
bioRxiv, 2021 Shimon M. Rosenthal +6 more
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Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking [PDF]
bioRxiv, 2017 The spatial organization of RNA within cells is a crucial factor in a wide range of biological functions, spanning all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods ...
Pornchai Kaewsapsak +4 more
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In‐Depth Cell‐Type‐Specific Proteome Landscape of the Brain from Human Amyloid‐β Overexpression Mouse Model [PDF]
Advanced ScienceAmyloid‐β (Aβ) plays a crucial role in Alzheimer's disease pathogenesis. Understanding how Aβ overexpression alters the proteome of individual brain cell types is essential but challenging due to the nature of brain tissue, which contains intermingled ...
Taekyung Ryu +6 more
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AgrC biotinylation inhibits Staphylococcus aureus infection. [PDF]
PLoS ONEStaphylococcus aureus (S. aureus) is a leading cause of nosocomial infections, particularly among antibiotic-resistant strains. S. aureus virulence is governed by the accessory gene regulator (Agr) quorum sensing (QS) system, which relies on AgrC, a two ...
Lijuan Qian +9 more
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STAR Protocols, 2020 Summary: The peroxidase APEX2 has been used widely for proximity biotinylation and subsequent proteomics analyses. However, the poor membrane permeability of the biotin phenol substrate and the inhibitory effect of peroxide on the enzyme’s activity has ...
Benedict Tan +5 more
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In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis [PDF]
, 2016 UnlabelledToxoplasma gondii is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into ...
Santhosh M. Nadipuram +7 more
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Expression and Site-Specific Biotinylation of Human Cytosolic 5′-Nucleotidase 1A in Escherichia coli [PDF]
Methods and ProtocolsAutoantibodies targeting cytosolic 5′-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren’s syndrome, and systemic lupus erythematosus.
Nataliya Slater +6 more
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Biotinylation of Cell Surface Proteins [PDF]
Bio-Protocol, 2012 Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their ...
Guo Huang
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Frontiers in Cellular and Infection Microbiology, 2022 Proximity labelling is a powerful and rapidly developing technology for exploring the interaction space and molecular environment of a protein of interest at the nanometre scale.
Vincent Geoghegan +2 more
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