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Proximity proteomics of primary cilia in human hypothalamic neurons
Macarelli V +5 more
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Unbiased Enrichment of Circulating Tumor Cells Via DNAzyme-Catalyzed Proximal Protein Biotinylation.
Nano letters (Print), 2022Circulating tumor cells (CTCs) are noninvasive biomarkers with great potential for assessing neoplastic diseases. However, the enrichment bias toward heterogeneous CTCs remains to be minimized.
Liang Chen +6 more
semanticscholar +1 more source
The Journal of Organic Chemistry, 2004
Inhibition of the 90 kDa heat shock proteins (Hsp90) represents a promising new chemotherapeutic approach for the treatment of several cancers. Hsp90 is essential to the survival of cancer cells and is inhibited by members of the ansamycin family of antibiotics.
Randell C, Clevenger +3 more
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Inhibition of the 90 kDa heat shock proteins (Hsp90) represents a promising new chemotherapeutic approach for the treatment of several cancers. Hsp90 is essential to the survival of cancer cells and is inhibited by members of the ansamycin family of antibiotics.
Randell C, Clevenger +3 more
openaire +2 more sources
Molecular Immunology, 1982
Purified human C3 was biotinylated using the biotinyl-N-hydroxysuccinimide imidoester (BNHS). Depending on the input of BNHS, from three to six molecules of biotin were incorporated per C3 molecule. The biotinyl-C3 retained over 90% of its specific hemolytic activity and when bound to sheep erythrocytes maintained its ability to adhere to human C3b ...
T A Gaither +5 more
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Purified human C3 was biotinylated using the biotinyl-N-hydroxysuccinimide imidoester (BNHS). Depending on the input of BNHS, from three to six molecules of biotin were incorporated per C3 molecule. The biotinyl-C3 retained over 90% of its specific hemolytic activity and when bound to sheep erythrocytes maintained its ability to adhere to human C3b ...
T A Gaither +5 more
openaire +3 more sources
Biotinylated Stealth® magnetoliposomes
Chemistry and Physics of Lipids, 2002Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the ...
Alois Steigel +5 more
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Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform.
Bioconjugate chemistry, 2021Isatoic anhydride (IA) has been shown to be a useful platform for quantifiable bioconjugation. The elaboration of a water-soluble isatoic anhydride-based platform with biotin offers readily quantifiable biotinylation reagents through nondestructive ...
Adam B. Fessler, A. Fowler, C. Ogle
semanticscholar +1 more source
Biotinylated peptides/proteins
Analytical Biochemistry, 1991The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of N epsilon modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin.
John S. Smith +2 more
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Synthesis of biotinylated diazinon: Lessons learned for biotinylation of thiophosphate esters
Tetrahedron Letters, 2018Abstract Biotinylation permits recovery of a molecule from a complex mixture, with commercially available streptavidin containing products (such as streptavidin-coated beads). As part of a larger effort to evaluate reagents capable of degrading diazinon, a thiophosphate insecticide, we pursued biotinylation of this molecule.
Brian R. McNaughton +2 more
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Reversible biotinylation of C1q with a cleavable biotinyl derivative
Journal of Immunological Methods, 1988Reversible biotinylation of human C1q without impairment of its physiologic functions has allowed us to develop a simple and rapid purification method for C1q receptor (C1qR). The biotinylating reagent, NHS-SS-biotin (Mr 606.7) contains an extended connector or cross-linker arm which limits steric hindrance and is bridged by a cleavable disulfide bond ...
Kenneth B.M. Reid +3 more
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Biotinylated gene therapy vectors
Expert Opinion on Biological Therapy, 2003The avidin-biotin system is a fundamental technology in biomedicine for immunolocalisation, imaging, nucleic acid blotting and protein labelling. This technology has recently been adapted for use in gene therapy vector applications to add proteins or cell-targeting ligands to non-viral and viral vectors. Two biotinylation technologies are being used in
Michael A. Barry +6 more
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