Results 231 to 240 of about 33,953 (257)
Some of the next articles are maybe not open access.
Current Protocols in Protein Science, 2010
AbstractSince its discovery in the first half of the twentieth century, the high‐affinity, noncovalent interaction between biotin (vitamin H) and the avian protein avidin (and its bacterial homologs) has been exploited for many diverse biotechnology applications.
openaire +5 more sources
AbstractSince its discovery in the first half of the twentieth century, the high‐affinity, noncovalent interaction between biotin (vitamin H) and the avian protein avidin (and its bacterial homologs) has been exploited for many diverse biotechnology applications.
openaire +5 more sources
[57] Biotinylated protein A in immunoassay
1990Publisher Summary This chapter describes the use of biotin-labeled protein A enzyme-linked immunoassay (ELISA) for simultaneous detection of JE antibody in the sera of humans, swine, and several other vertebrates using the same reagents. Swine sera were collected from endemic and nonendemic areas of Japan.
Hshi-Chi Chang +2 more
openaire +3 more sources
Synthesis of biotinylated thymocartins
Journal of Labelled Compounds and Radiopharmaceuticals, 2000Four biotinylated derivatives of Arg-Lys-Asp-Val immunostimulating tetrapeptide labelled either at the α-amino group of arginyl or the ϵ-amino moiety for lysyl residue have been synthesized for biological studies using classical methods. Copyright © 2000 John Wiley & Sons, Ltd.
Ádám Demeter, Istvan Schon, Olga Nyeki
openaire +2 more sources
Fragmentation of biotinylated cyclic peptides
Rapid Communications in Mass Spectrometry, 2004AbstractElectrospray ionization coupled with tandem mass spectrometry (MS/MS) was used to determine the preferred binding site(s) of biotin NHS ester with a series of cyclic peptides with antibiotic properties. The peptides investigated are polymyxins, cyclic peptides produced by Bacillus polymyxa. In spite of the 1:1 stoichiometry used in the labeling
Chris R. Taitt +2 more
openaire +3 more sources
1994
Using the characteristic of a high-affinity complex between avidin and biotin, biotinylated antibodies have wide applications in various immunochemical assays, especially where signal amplification is required. A method is described here for the biotinylation of immunoglobulins.
openaire +5 more sources
Using the characteristic of a high-affinity complex between avidin and biotin, biotinylated antibodies have wide applications in various immunochemical assays, especially where signal amplification is required. A method is described here for the biotinylation of immunoglobulins.
openaire +5 more sources
2014
Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (244 Da), it can be conjugated to many proteins without altering their biological activities. The biotinylated molecule can be detected in ELISA, dot blot, or Western blot methods (see Western Blotting using ...
openaire +3 more sources
Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (244 Da), it can be conjugated to many proteins without altering their biological activities. The biotinylated molecule can be detected in ELISA, dot blot, or Western blot methods (see Western Blotting using ...
openaire +3 more sources
Effective Methods for the Biotinylation of Azamacrocycles
The Journal of Organic Chemistry, 2007The biotin-(strept)avidin interaction remains a gold standard of model biological recognition events. The biotinylation of azamacrocycles permits the investigation of signal transduction between this recognition event and the metal center of an azamacrocycle complex, of wide potential interest in biosensing. There are no generally applicable procedures
Michael Watkinson +3 more
openaire +3 more sources
4‐Thiouridine‐Enhanced Peroxidase‐Generated Biotinylation of RNA
ChemBioChem, 2020Peroxidase‐generated proximity labeling is in widespread use to study subcellular proteomes and the protein interaction networks in living cells, but the development of subcellular RNA labeling is limited.
Jinguo Huang +9 more
semanticscholar +1 more source
1990
Publisher Summary This chapter discusses the procedures by which researchers have incorporated biotin moieties into proteins. Several laboratories have reported the use of an avidin-based assay for detecting the extent of biotinylation. An effective method for detecting the average number of biotin groups per protein molecule would be to first remove
Meir Wilchek, Edward A. Bayer
openaire +2 more sources
Publisher Summary This chapter discusses the procedures by which researchers have incorporated biotin moieties into proteins. Several laboratories have reported the use of an avidin-based assay for detecting the extent of biotinylation. An effective method for detecting the average number of biotin groups per protein molecule would be to first remove
Meir Wilchek, Edward A. Bayer
openaire +2 more sources
Biotinylated Probes in Colony Hybridization
1998Colony hybridization is a procedure that allows the detection of cells containing nucleic acid sequences of interest (1). In this method, microbial colonies grown on, or transferred to, a supporting membrane are lysed and their nucleic adds denatured to single strands and fixed in place on the membrane.
openaire +5 more sources