Results 241 to 250 of about 49,644 (261)

Affinity chromatography with biotinylated RNAs

1990
Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand.
S W, Ruby, S E, Goelz, Z, Hostomsky, J N, Abelson   +3 more
openaire   +2 more sources

Biotinylated peptides/proteins. II. Identification of biotinylated lysyl phenylthiohydantoins.

Analytical biochemistry, 1991
The identification and characterization of biotinylated lysyl residues in a polypeptide chain by automated sequence analysis is described. An in depth analytical study was conducted for the delineation of N epsilon modification of lysyl residues with N-hydroxysuccinimide esters of biotin and 6-aminohexanoic biotin.
J S, Smith, B T, Miller, A, Kurosky
openaire   +1 more source

Solid‐phase biotinylation of antibodies

Journal of Molecular Recognition, 2004
AbstractBiotinylation is an established method of labeling antibody molecules for several applications in life science research. Antibody functional groups such as amines, cis hydroxyls in carbohydrates or sulfhydryls may be modified with a variety of biotinylation reagents.
Elizabeth, Strachan, A Krishna, Mallia, Joanna M, Cox, Babu, Antharavally, Surbhi, Desai, Laura, Sykaluk, Valerie, O'Sullivan, Peter A, Bell   +7 more
openaire   +2 more sources

Synthesis of biotinylated diazinon: Lessons learned for biotinylation of thiophosphate esters

Tetrahedron Letters, 2018
Abstract Biotinylation permits recovery of a molecule from a complex mixture, with commercially available streptavidin containing products (such as streptavidin-coated beads). As part of a larger effort to evaluate reagents capable of degrading diazinon, a thiophosphate insecticide, we pursued biotinylation of this molecule.
Kyle G. Nottingham, Andrew McNally, Brian R. McNaughton   +2 more
openaire   +1 more source

Biotinylated Probes in Colony Hybridization

1998
Colony hybridization is a procedure that allows the detection of cells containing nucleic acid sequences of interest (1). In this method, microbial colonies grown on, or transferred to, a supporting membrane are lysed and their nucleic adds denatured to single strands and fixed in place on the membrane.
openaire   +4 more sources

Kinase-Catalyzed Biotinylation.

Current protocols in chemical biology
Kinase-catalyzed protein phosphorylation plays an essential role in a variety of biological processes. Methods to detect phosphoproteins and phosphopeptides in cellular mixtures will aid in cell biological and signaling research. Our laboratory recently discovered the utility of γ-modified ATP analogues as tools for studying phosphorylation ...
Chamara, Senevirathne, Keith D, Green, Mary Kay H, Pflum   +2 more
openaire   +1 more source

Reversible biotinylation of C1q with a cleavable biotinyl derivative

Journal of Immunological Methods, 1988
Berhane Ghebrehiwet, Steven Bossone, Anna Erdei, Kenneth B.M. Reid   +3 more
openaire   +1 more source

Biotinylated peptides/proteins

Analytical Biochemistry, 1991
John S. Smith, Brian T. Miller, Susan L. Knock, Alexander Kurosky   +3 more
openaire   +2 more sources

Biotinylated agarose

Cold Spring Harbor Protocols, 2006
openaire   +1 more source

Avidin binding of biotinylated corticotropins

Biochemistry, 1983
H, Romovacek, F M, Finn, K, Hofmann
openaire   +2 more sources