Results 241 to 250 of about 33,953 (257)

In vivo enzymatic protein biotinylation

Biomolecular Engineering, 1999
Biotin is biologically active only when protein-bound and is covalently attached to a class of important metabolic enzymes, the biotin carboxylases and decarboxylases. Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different organisms.
John E. Cronan, Anne Chapman-Smith
openaire   +3 more sources

[20] Hybrid selection of rnRNA with biotinylated DNA

Gene Analysis Techniques, 1990
A novel, convenient, and highly efficient hybrid selection procedure is described. The method utilizes the polymerase chain reaction (PCR) in which one of two primers is biotinylated at the 5'-terminus. The concentration of the biotinylated primer is 100 times that of the other to synthesize biotinylated single-stranded DNA (asymmetric PCR).
Sidney Pestka, Jaemog Soh
openaire   +6 more sources

Selections on Biotinylated Antigens

2001
Phage antibody library selections on peptides or proteins are usually carried out using antigens directly coated on a plastic surface (e.g. Petri dishes, microtiter plate well, immunotubes, Chapter 9). This straightforward method is easy to perform and has been shown to be very successful for a diverse set of antigens (for review see Winter et al. 1994)
Patrick Chames, Paula Henderikx, Hennie R. Hoogenboom   +2 more
openaire   +2 more sources

Affinity chromatography with biotinylated RNAs

1990
Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand.
John Abelson, S.E. Goelz, Z. Hostomsky, S.W. Ruby   +3 more
openaire   +3 more sources

A proximity-dependent biotinylation map of a human cell

Nature, 2021
Christopher D. Go, Christopher D. Go, J. Knight, Archita Rajasekharan, B. Rathod, G. Hesketh, K. Abe, K. Abe, J. Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y Zhu, Evelyn Popiel, Jean-Philippe Lambert, Jean-Philippe Lambert, É. Coyaud, É. Coyaud, Sally W T Cheung, D. Rajendran, Cassandra J. Wong, H. Antonicka, L. Pelletier, L. Pelletier, B. Raught, B. Raught, Alexander F. Palazzo, E. Shoubridge, A. Gingras, A. Gingras   +28 more
semanticscholar   +1 more source

Syntheses of biotinylated and dethiobiotinylated insulins

Biochemistry, 1984
The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistry of d-biotin, is the major isomer.
Frances M. Finn, P. K. Mishra, Klaus Hofmann, Wei Jun Zhang, Hana Romovacek, Aksel A. Bothner-By   +5 more
openaire   +3 more sources

Solid‐phase biotinylation of antibodies

Journal of Molecular Recognition, 2004
AbstractBiotinylation is an established method of labeling antibody molecules for several applications in life science research. Antibody functional groups such as amines, cis hydroxyls in carbohydrates or sulfhydryls may be modified with a variety of biotinylation reagents.
Elizabeth Strachan, Joanna M. Cox, Peter A. Bell, Laura Sykaluk, Babu Siddegowda Antharavally, Valerie O'Sullivan, A. Krishna Mallia, Surbhi Desai   +7 more
openaire   +3 more sources

Biotinylation Reagents

2008
Publisher Summary The highly specific interaction of avidin with the small vitamin biotin can be a useful tool in designing assay, detection, and targeting systems for biological analytes. The extraordinary affinity of (strept)avidin's interaction with biotin allows biotin-containing molecules in complex mixtures to be discretely bound with (strept ...
openaire   +2 more sources

Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.

Current Opinion in Chemical Biology, 2019
A. Gingras, K. Abe, B. Raught
semanticscholar   +1 more source

Avidin binding of biotinylated corticotropins

Biochemistry, 1983
Hana Romovacek, Frances M. Finn, Klaus Hofmann   +2 more
openaire   +3 more sources