Results 21 to 30 of about 33,953 (257)
Nature Communications, 2022 Proteolysis-targeting chimaeras (PROTACs) as well as molecular glues such as immunomodulatory drugs (IMiDs) and indisulam are drugs that induce interactions between substrate proteins and an E3 ubiquitin ligases for targeted protein degradation. Here, we
S. Yamanaka +8 more
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Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome
Frontiers in Molecular Biosciences, 2022 In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms.
Ugo Dionne, A. Gingras
semanticscholar +1 more source
Frontiers in Microbiology, 2020 Liver fluke proteins, including excretory-secretory products (ESPs) and tegument proteins, are critical for the pathogenesis, nutrient metabolism, etiology and immune response of liver cancer. To understand the functions of various proteins in Clonorchis
Yunliang Shi +10 more
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Streptavidin-Saporin: Converting Biotinylated Materials into Targeted Toxins
Toxins, 2023 Streptavidin-Saporin can be considered a type of ‘secondary’ targeted toxin. The scientific community has taken advantage of this conjugate in clever and fruitful ways using many kinds of biotinylated targeting agents to send saporin into a cell selected
Leonardo R. Ancheta +4 more
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Proximity‐dependent biotinylation approaches to study apicomplexan biology
Molecular Microbiology, 2021 In the last 10 years, proximity‐dependent biotinylation (PDB) techniques greatly expanded the ability to study protein environments in the living cell that range from specific protein complexes to entire compartments.
Jessica Kimmel +3 more
semanticscholar +1 more source
In vivo discovery of RNA proximal proteins via proximity-dependent biotinylation
RNA Biology, 2021 RNA molecules function as messenger RNAs (mRNAs) that encode proteins and noncoding transcripts that serve as adaptor molecules, structural components, and regulators of genome organization and gene expression.
Xianzhi Lin +4 more
semanticscholar +1 more source
Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches*
Molecular & Cellular Proteomics, 2020 Proximity-dependent biotinylation approaches such as BioID and APEX overcome classical limitations of biochemical purification and have gained widespread use in recent years for revealing cellular neighborhoods.
Payman Samavarchi-Tehrani +2 more
semanticscholar +1 more source
Journal of the American Society for Mass Spectrometry, 2021 Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-bead digestion in numerous biological applications.
Haorong Li +4 more
semanticscholar +1 more source
Biotinylated Cyclooligosaccharides for Paclitaxel Solubilization [PDF]
Molecules, 2018 The poor water solubility of paclitaxel causes significant problems in producing cancer therapeutic formulations. Here, we aimed to solubilize paclitaxel using biocompatible cyclic carbohydrates. Generally recognized as safe, labeled β-cyclodextrin (β-CD), a cyclic α-1,4-glucan consisting of seven glucoses, was prepared, and bio-sourced ...
Seunho Jung, Eunae Cho
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Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source