Results 31 to 40 of about 54,144 (311)
Adenovirus Fibers as Ultra-Stable Vehicles for Intracellular Nanoparticle and Protein Delivery
Biomolecules, 2022 Protein-based carriers are promising vehicles for the intracellular delivery of therapeutics. In this study, we designed and studied adenovirus protein fiber constructs with potential applications as carriers for the delivery of protein and nanoparticle ...
Chrysoula Kokotidou +6 more
doaj +1 more source
Frontiers in Microbiology, 2020 Liver fluke proteins, including excretory-secretory products (ESPs) and tegument proteins, are critical for the pathogenesis, nutrient metabolism, etiology and immune response of liver cancer. To understand the functions of various proteins in Clonorchis
Yunliang Shi +10 more
doaj +1 more source
The generality of kinase-catalyzed biotinylation [PDF]
Bioorganic & Medicinal Chemistry, 2015 Kinase-catalyzed protein phosphorylation is involved in a wide variety of cellular events. Development of methods to monitor phosphoproteins in normal and diseased states is critical to fully characterize cell signaling. Towards phosphoprotein analysis tools, our lab reported kinase-catalyzed labeling where γ-phosphate modified ATP analogs are utilized
Chamara Senevirathne +4 more
openalex +4 more sources
Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source
Biotinylated Platelets: A Promising Labeling Technique?
Transfusion Medicine Reviews, 2023 Labeling of platelets (PLTs) is essential for research purposes, in order to measure the recovery and survival of transfused PLTs in vivo. Biotinylation is a promising new alternative to the gold standard of radioactive labeling. This review highlights 4 key publications that provide significant insights into biotin-labeled PLTs (bioPLTs).
Stefan F. van Wonderen +6 more
openaire +3 more sources
Probing the environment of emerin by Enhanced ascorbate peroxidase 2 (APEX2)-mediated proximity labeling. [PDF]
, 2020 Emerin is one of the best characterized proteins of the inner nuclear membrane, but can also occur at the level of the endoplasmic reticulum. We now use enhanced ascorbate peroxidase 2 (APEX2) to probe the environment of emerin.
James, C. +4 more
core +1 more source
Dynamin- and Rab5-Dependent Endocytosis of a Ca2+-Activated K+ Channel, KCa2.3 [PDF]
, 2012 Regulation of the number of ion channels at the plasma membrane is a critical component of the physiological response. We recently demonstrated that the Ca2+-activated K+ channel, KCa2.3 is rapidly endocytosed and enters a Rab35- and EPI64C-dependent ...
Agustin Guerrero-Hernandez +5 more
core +10 more sources
Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches*
Molecular & Cellular Proteomics, 2020 Proximity-dependent biotinylation approaches such as BioID and APEX overcome classical limitations of biochemical purification and have gained widespread use in recent years for revealing cellular neighborhoods.
Payman Samavarchi-Tehrani +2 more
semanticscholar +1 more source
Proximity‐dependent biotinylation approaches to study apicomplexan biology
Molecular Microbiology, 2021 In the last 10 years, proximity‐dependent biotinylation (PDB) techniques greatly expanded the ability to study protein environments in the living cell that range from specific protein complexes to entire compartments.
Jessica Kimmel +3 more
semanticscholar +1 more source
Novel components of the Toxoplasma inner membrane complex revealed by BioID. [PDF]
, 2015 UNLABELLED:The inner membrane complex (IMC) of Toxoplasma gondii is a peripheral membrane system that is composed of flattened alveolar sacs that underlie the plasma membrane, coupled to a supporting cytoskeletal network. The IMC plays important roles in
Bell, Hannah N +11 more
core +3 more sources