Results 31 to 40 of about 33,953 (257)
ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells
bioRxiv, 2021 Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E.
Xiyan Zhao +9 more
semanticscholar +1 more source
Mapping axon initial segment structure and function by multiplexed proximity biotinylation
Nature Communications, 2020 Axon initial segments (AISs) generate action potentials and regulate the polarized distribution of proteins, lipids, and organelles in neurons. While the mechanisms of AIS Na+ and K+ channel clustering are understood, the molecular mechanisms that ...
Hamdan Hamdan +11 more
semanticscholar +1 more source
Journal of Biochemistry (Tokyo), 2021 Recent advances in biotinylation-based proximity labeling (PL) have opened up new avenues for mapping the protein composition of cellular compartments and protein complexes in living cells at high spatiotemporal resolution.
Tomoya Niinae, Y. Ishihama, Koshi Imami
semanticscholar +1 more source
Application of Skyline for Analysis of Protein–Protein Interactions In Vivo
Molecules, 2021 Quantitative and qualitative analyses of cell protein composition using liquid chromatography/tandem mass spectrometry are now standard techniques in biological and clinical research.
Arman Kulyyassov
doaj +1 more source
bioRxiv, 2017 The spatial organization of RNA within cells is a crucial factor in a wide range of biological functions, spanning all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods ...
Pornchai Kaewsapsak +4 more
openalex +2 more sources
Cell-Type-Specific Proteomics: A Neuroscience Perspective
Proteomes, 2018 Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and ...
Rashaun S. Wilson, Angus C. Nairn
doaj +1 more source
Synthesis of biotinylated OSW-1 [PDF]
Bioorganic & Medicinal Chemistry Letters, 2009 OSW-1 is a highly potent anticancer natural saponin with an unknown mode of action. To determine its cellular target(s) biotinylated OSW-1 was successfully synthesized in nine steps.
Changgang Lou +4 more
openaire +3 more sources
Cell Reports, 2022 Summary: Intrahepatic cholangiocarcinoma (ICC) contains abundant myofibroblasts derived from hepatic stellate cells (HSCs) through an activation process mediated by TGF-β.
Liankang Sun +15 more
doaj
Tissue Specific Labeling in Proteomics
Proteomes, 2017 Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in ...
Evelyn Ramberger, Gunnar Dittmar
doaj +1 more source
β-Actin mRNA interactome mapping by proximity biotinylation [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance Transport of specific mRNAs to defined sites in the cytoplasm allows local protein production and contributes to cell polarity, embryogenesis, and neuronal function.
Joyita Mukherjee +6 more
openalex +2 more sources