Results 31 to 40 of about 49,644 (261)

Biotin attenuates heat shock factor 4b transcriptional activity by lysine 444 biotinylation

Biochemistry and Biophysics Reports, 2022
Genetic mutations in HSF4 cause congenital cataracts. HSF4 exhibits both positive and negative regulation on the transcription of heat shock and non-heat shock proteins during lens development, and its activity is regulated by posttranslational ...
Longjun Yan, Jing Li, Jialin Hu, Junwei Qu, Kejia Li, Mingli Wang, Shuang-Shuang An, Cun-cun Ke, Hui Li, Fengling Yuan, Weikai Guo, Mengyue Hu, Jing Zhang, Zhengyan Yang, Hongmei Mu, Fengyan zhang, Jun Zhang, Xiukun Cui, Yanzhong Hu   +18 more
doaj   +1 more source

In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis. [PDF]

, 2016
UnlabelledToxoplasma gondii is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into ...
Bell, Hannah N, Bradley, Peter J, Coppens, Isabelle, Kim, Elliot W, Lin, Andrew H, Nadipuram, Santhosh M, Vashisht, Ajay A, Wohlschlegel, James A   +7 more
core   +2 more sources

Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells

STAR Protocols, 2022
Summary: Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset
Serife Ayaz-Guner, Mustafa Burak Acar, Dudu Boyvat, Huseyin Guner, Habibe Bozalan, Melis Güzel, Selin Kübra Yıldır, Nilay Altınsoy, Fatma Fındık, Musa Karakükçü, Servet Özcan   +10 more
doaj   +1 more source

Oxysterol-Binding Protein-1 (OSBP1) Modulates Processing and Trafficking of the Amyloid Precursor Protein [PDF]

, 2008
BACKGROUND Evidence from biochemical, epidemiological and genetic findings indicates that cholesterol levels are linked to amyloid-β (Aβ) production and Alzheimer's disease (AD). Oxysterols, which are cholesterol-derived ligands of the liver X receptors
Abraham, Carmela R., Chen, Ci-Di, Cordy, Joanna M., Guillily, Maria, Ray, William J., Seabrook, Guy R., Suon, Sokreine, Wolozin, Benjamin, Zerbinatti, Celina V.   +8 more
core   +4 more sources

Synthesis of biotinylated OSW-1 [PDF]

Bioorganic & Medicinal Chemistry Letters, 2009
OSW-1 is a highly potent anticancer natural saponin with an unknown mode of action. To determine its cellular target(s) biotinylated OSW-1 was successfully synthesized in nine steps.
Ying, Kang, Changgang, Lou, Kausar Begam Riaz, Ahmed, Peng, Huang, Zhendong, Jin   +4 more
openaire   +2 more sources

Reduced neurosteroid potentiation of GABAA receptors in epilepsy and depolarized hippocampal neurons [PDF]

, 2020
OBJECTIVE: Neurosteroids regulate neuronal excitability by potentiating γ-aminobutyric acid type-A receptors (GABARs). In animal models of temporal lobe epilepsy, the neurosteroid sensitivity of GABARs is diminished and GABAR subunit composition is ...
Jansen, Laura A, Joshi, Suchitra, Kapur, Jaideep, Roden, William H   +3 more
core   +2 more sources

Identification of PLXDC1 and PLXDC2 as the transmembrane receptors for the multifunctional factor PEDF. [PDF]

, 2014
Pigment Epithelium Derived Factor (PEDF) is a secreted factor that has broad biological activities. It was first identified as a neurotrophic factor and later as the most potent natural antiangiogenic factor, a stem cell niche factor, and an inhibitor of
Al-Ubaidi, Muayyad, Cheng, Guo, Deng, Jun, Kassai, Miki, Kawaguchi, Riki, Sun, Hui, Ter-Stepanian, Mariam, Zhong, Ming   +7 more
core   +1 more source

Specific biotinylation of IMP dehydrogenase [PDF]

Bioorganic & Medicinal Chemistry Letters, 2011
IMP dehydrogenase (IMPDH) catalyzes a critical step in guanine nucleotide biosynthesis. IMPDH also has biological roles that are distinct from its enzymatic function. We report a biotin-linked reagent that selectively labels IMPDH and is released by dithiothreitol. This reagent will be invaluable in elucidating the moonlighting functions of IMPDH.
B Christopher, Hoefler, Deviprasad R, Gollapalli, Lizbeth, Hedstrom   +2 more
openaire   +2 more sources

Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β. [PDF]

, 2016
Mutations in human CLC-1 chloride channel are associated with the skeletal muscle disorder myotonia congenita. The disease-causing mutant A531V manifests enhanced proteasomal degradation of CLC-1.
Chen, Shu-Ching, Chen, Tsung-Yu, Hsieh, Hsin-Ying, Huang, Jing-Jia, Peng, Yi-Jheng, Tang, Chih-Yung, Wu, Chia-Ying, Wu, Hao-Han   +7 more
core   +1 more source

Mechanisms Governing Precise Protein Biotinylation

Trends in Biochemical Sciences, 2017
Protein biotinylation is a key post-translational modification found throughout the living world. The covalent attachment of a biotin cofactor onto specific metabolic enzymes is essential for their activity. This modification is distinctive, in that it is carried out by a single enzyme: biotin protein ligase (BPL), an enzyme that is able to biotinylate
Louise M. Sternicki, Kate L. Wegener, John B. Bruning, Grant W. Booker, Steven W. Polyak   +4 more
openaire   +4 more sources