Results 41 to 50 of about 33,953 (257)
Proximity Utilizing Biotinylation of Nuclear Proteins in vivo
Central Asian Journal of Global Health, 2015 Introduction. The human genome consists of roughly 30,000 genes coding for over 500,000 different proteins, of which more than 10,000 proteins can be produced by the cell at any given time (the cellular “proteome”). It has been estimated that over 80% of
Arman Kulyyassov +3 more
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Frontiers in Pharmacology, 2020 Clavulanic acid (CLV) and amoxicillin, frequently administered in combination, can be independently involved in allergic reactions. Protein haptenation with β-lactams is considered necessary to activate the immune system.
Ángela Martín-Serrano +15 more
doaj +1 more source
Specific biotinylation of IMP dehydrogenase [PDF]
Bioorganic & Medicinal Chemistry Letters, 2011 IMP dehydrogenase (IMPDH) catalyzes a critical step in guanine nucleotide biosynthesis. IMPDH also has biological roles that are distinct from its enzymatic function. We report a biotin-linked reagent that selectively labels IMPDH and is released by dithiothreitol. This reagent will be invaluable in elucidating the moonlighting functions of IMPDH.
Lizbeth Hedstrom +2 more
openaire +3 more sources
Frontiers in Bioengineering and Biotechnology, 2017 Protein modification by covalent coupling of small ligands or markers is an important prerequisite for the use of proteins in many applications. Well-known examples are the use of proteins with fluorescent markers in many in vivo experiments or the ...
Regina Fraas +2 more
doaj +1 more source
Assembly of the β4-Integrin Interactome Based on Proximal Biotinylation in the Presence and Absence of Heterodimerization* [PDF]
Molecular & Cellular Proteomics, 2018 This study characterized the β4-integrin interacting proteome using BioID proximity-dependent biotinylation in epithelial MDCK cells. The analysis identified several novel type II hemidesmosome (HD)-associated proteins and revealed potential connecting ...
Satu-Marja Myllymäki +7 more
openalex +2 more sources
Angewandte Chemie, EarlyView.We introduce two methods for incorporating carbazolyl cyanobenzenes onto peptides: a C‐terminus decarboxylative functionalization and a Cys‐selective SNAr reaction. Both conjugates exhibit excellent photoredox/energy‐transfer properties, enabling their use for C‐terminal alkynylation and Cys‐selective thiol‐ene reactions on peptides, as well as ...
Xing‐Yu Liu +4 more
wiley +2 more sources
Site directed biotinylation of filamentous phage structural proteins
Virology Journal, 2011 Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E.
Smelyanski Larisa, Gershoni Jonathan M
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Angewandte Chemie, EarlyView.To monitor small molecule‐induced changes in protein polyubiquitination in an unbiased manner, we employ enrichment through tandem ubiquitin binding entities coupled to LC‐MS/MS analysis (TUBE‐MS). By applying the workflow to PROTAC, p97‐ and deubiquitinase‐inhibitor treatment of cells, we demonstrate its broad applicability and reveal the induction of
Siska Führer +6 more
wiley +2 more sources
eLife, 2017 The cytosol-facing membranes of cellular organelles contain proteins that enable signal transduction, regulation of morphology and trafficking, protein import and export, and other specialized processes.
Victoria Hung +7 more
semanticscholar +1 more source
PLoS ONE, 2020 Toxoplasma gondii is an obligate intracellular parasite which is capable of establishing life-long chronic infection in any mammalian host. During the intracellular life cycle, the parasite secretes an array of proteins into the parasitophorous vacuole ...
S. Nadipuram +4 more
semanticscholar +1 more source