Results 41 to 50 of about 49,644 (261)
Sensors, 2018 We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen +5 more
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A Peptide Core Motif for Binding to Heterotrimeric G Protein α Subunits [PDF]
, 2005 Recently, in vitro selection using mRNA display was used to identify a novel peptide sequence that binds with high affinity to G{alpha}i1. The peptide was minimized to a 9-residue sequence (R6A-1) that retains high affinity and specificity for the GDP ...
Adhikari, Anirban +4 more
core +2 more sources
Off-the-shelf proximity biotinylation for interaction proteomics
Nature Communications, 2021 Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling ...
Irene Santos-Barriopedro +2 more
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Structure of the full-length TRPV2 channel by cryo-EM. [PDF]
, 2016 Transient receptor potential (TRP) proteins form a superfamily Ca(2+)-permeable cation channels regulated by a range of chemical and physical stimuli. Structural analysis of a 'minimal' TRP vanilloid subtype 1 (TRPV1) elucidated a mechanism of channel ...
Cohen, Matthew R +6 more
core +2 more sources
Journal of Experimental Life Science, 2019 Protein has been known as an important macromolecule which has a vital role among the living organism. One of the most interesting protein is Arc1p, which is a yeast-specific tRNA-binding protein.
Restu Nugraha +2 more
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Life, 2022 In vivo biotinylation using wild-type and mutants of biotin ligases is now widely applied for the study of cellular proteomes. The commercial availability of kits for the highly efficient purification of biotinylated proteins and their excellent ...
Darkhan Kanayev +5 more
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Site directed biotinylation of filamentous phage structural proteins
Virology Journal, 2011 Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E.
Smelyanski Larisa, Gershoni Jonathan M
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AirID, a novel proximity biotinylation enzyme, for analysis of protein–protein interactions
eLife, 2020 Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism.
Kohki Kido +8 more
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Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore +54 more
core +1 more source
Proximity Utilizing Biotinylation of Nuclear Proteins in vivo
Central Asian Journal of Global Health, 2015 Introduction. The human genome consists of roughly 30,000 genes coding for over 500,000 different proteins, of which more than 10,000 proteins can be produced by the cell at any given time (the cellular “proteome”). It has been estimated that over 80% of
Arman Kulyyassov +3 more
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