Results 51 to 60 of about 33,953 (257)

Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

Life, 2022
In vivo biotinylation using wild-type and mutants of biotin ligases is now widely applied for the study of cellular proteomes. The commercial availability of kits for the highly efficient purification of biotinylated proteins and their excellent ...
Darkhan Kanayev, Diana Abilmazhenova, Ilyas Akhmetollayev, Aliya Sekenova, Vyacheslav Ogay, Arman Kulyyassov   +5 more
doaj   +1 more source

Addendum: Biotinylation by antibody recognition—a method for proximity labeling [PDF]

Nature Methods, 2018
Daniel Z. Bar, Kathleen Atkatsh, Urraca Tavarez, Michael R. Erdos, Yosef Gruenbaum, Francis S. Collins   +5 more
openalex   +2 more sources

Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking

BioTechniques, 2011
We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which ...
Faraz Ahmad, Sarah Kate Coleman, Kai Kaila, Peter Blaesse   +3 more
doaj   +1 more source

Force dependent biotinylation of myosin IIA by α-catenin tagged with a promiscuous biotin ligase. [PDF]

PLoS ONE, 2015
Tissues and organs undergo constant physical perturbations and individual cells must respond to mechanical forces to maintain tissue integrity. However, molecular interactions underlying mechano-transduction are not fully defined at cell-cell junctions ...
Shuji Ueda, Alexandra M Blee, Katherine G Macway, Derrick J Renner, Soichiro Yamada   +4 more
doaj   +1 more source

Profiling the interactome of oligonucleotide drugs by proximity biotinylation.

Nature Chemical Biology
Drug-ID is a novel method applying proximity biotinylation to identify drug-protein interactions inside living cells. The covalent conjugation of a drug with a biotin ligase enables targeted biotinylation and identification of the drug-bound proteome. We
Alfred Hanswillemenke, D. T. Hofacker, M. Sorgenfrei, Carolin Fruhner, M. Franz‐Wachtel, Dirk Schwarzer, Boris Maček, Thorsten Stafforst   +7 more
semanticscholar   +1 more source

Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation

Journal of Cell Biology, 2020
Chastney et al. use multiplexed proximity-dependent labeling to define the topology of integrin adhesion complexes. The data support current models of adhesome architecture and provide a resource to examine proximal relationships within the adhesome and ...
Megan R. Chastney, C. Lawless, J. Humphries, Stacey Warwood, Matthew C. Jones, David Knight, Claus Jørgensen, M. Humphries   +7 more
semanticscholar   +1 more source

Biotinylation as a tool to enhance the uptake of small molecules in Gram-negative bacteria.

PLoS ONE, 2021
Antibiotic resistance is a major public health concern. The shrinking selection of effective antibiotics and lack of new development is making the situation worse.
Ankit Pandeya, Ling Yang, Olaniyi Alegun, Chamikara Karunasena, Chad Risko, Zhenyu Li, Yinan Wei   +6 more
doaj   +2 more sources

Histone biotinylation inCandida albicans [PDF]

FEMS Yeast Research, 2013
Candida albicans is an opportunistic fungal pathogen in humans. It is a polymorphic fungus: it can live as yeasts, hyphae, or pseudohyphae. Biotin is required for cell growth and fatty acid metabolism because it is used as a cofactor for carboxylases such as acetyl-CoA carboxylase, and pyruvate carboxylase.
Hasim, Sahar, Tati, Swetha, Madayiputhiya, Nandakumar, Nandakumar, Renu, Nickerson, Kenneth W.   +4 more
openaire   +3 more sources

PLUS: Primary Layer for Universal Sensing Enabling Improved Immunocapture of Biomarkers in Clinical Scenarios

Advanced Healthcare Materials, EarlyView.
Primary Layer for Universal Sensing (PLUS), a universal coating for bioprobe immobilization across diverse substrates, is introduced. Unlike conventional methods, PLUS is directly grown from dopamine and avidin, creating a roughened surface with abundant biotin‐binding sites.
Nayoung Son, Chorok Byeon, Haejin Jeong, Hyeonha Jang, Jun Seok Park, Seonki Hong   +5 more
wiley   +1 more source

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

Sensors, 2018
We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen, Hanim Kim, Dasom Kim, Jin Oh Lee, Myung Chan Gye, Young-Pil Kim   +5 more
doaj   +1 more source