Results 51 to 60 of about 49,644 (261)

Force dependent biotinylation of myosin IIA by α-catenin tagged with a promiscuous biotin ligase. [PDF]

PLoS ONE, 2015
Tissues and organs undergo constant physical perturbations and individual cells must respond to mechanical forces to maintain tissue integrity. However, molecular interactions underlying mechano-transduction are not fully defined at cell-cell junctions ...
Shuji Ueda, Alexandra M Blee, Katherine G Macway, Derrick J Renner, Soichiro Yamada   +4 more
doaj   +1 more source

Restoration of CFTR function in patients with cystic fibrosis carrying the F508del-CFTR mutation [PDF]

, 2014
Restoration of BECN1/Beclin 1-dependent autophagy and depletion of SQSTM1/p62 by genetic manipulation or autophagy-stimulatory proteostasis regulators, such as cystamine, have positive effects on mouse models of human cystic fibrosis (CF). These measures
Amin R, Andrea Venerando, Angela Sepe, Anil Mehta, Anna Bossi, Anna Zolin, Antonella Tosco, Boyle MP BS, Carlo A Leone, Daniela De Stefano, Donaldson S., Fabiola De Gregorio, Fischer H, Gianni Bona, Gibson LE, Giuseppe De Rosa, Guido Kroemer, Illek B, Jayaraman S, Kerem B, Kerem Eithan HMO, Laura Salvadori, Legssyer R, Lorenzo A Pinna, Luigi Maiuri, Lukacs G, Maria C Maiuri, Massimo Pettoello-Mantovani, Masvidal L, Pharmaceuticals Cylene, Pharmaceuticals Cylene, Rosa Grassia, Speranza Esposito, Standardization of Spirometry, Stefano Guido, Stern M, Valeria R Villella, Valeria Raia, van Doorninck JH, Villella VR   +39 more
core   +4 more sources

Biotinylated Primary Amines

Proceedings of The 14th International Electronic Conference on Synthetic Organic Chemistry, 2010
In the frame of a biotinylation program of biologically active amines we synthesized two amides from 2-phenetylamine and 3,4-dimethoxybenzylamine, that were fully characterized by means of multinuclear NMR spectroscopy.
Dionisia Sanz, Clarissa Frizzo, José Elguero, Rosa Claramunt   +3 more
openaire   +1 more source

Automated Solid-Phase Protein Modification with Integrated Enzymatic Digest for Reaction Validation: Application of a Compartmented Microfluidic Reactor for Rapid Optimization and Analysis of Protein Biotinylation

Frontiers in Bioengineering and Biotechnology, 2017
Protein modification by covalent coupling of small ligands or markers is an important prerequisite for the use of proteins in many applications. Well-known examples are the use of proteins with fluorescent markers in many in vivo experiments or the ...
Regina Fraas, Juliane Diehm, Matthias Franzreb   +2 more
doaj   +1 more source

Coming together to define membrane contact sites. [PDF]

, 2019
Close proximities between organelles have been described for decades. However, only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell ...
De Matteis, Maria Antonietta, Emr, Scott, Giordano, Francesca, Hajnóczky, György, Kornmann, Benoît, Lackner, Laura L., Levine, Tim P., Pellegrini, Luca, Reinisch, Karin, Rizzuto, Rosario, Schuldiner, Maya, Scorrano, Luca, Simmen, Thomas, Stenmark, Harald, Ungermann, Christian   +14 more
core   +7 more sources

Tissue Specific Labeling in Proteomics

Proteomes, 2017
Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in ...
Evelyn Ramberger, Gunnar Dittmar
doaj   +1 more source

Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants

Bio-Protocol, 2022
Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin.
Nicole Gibbs, Shih- Su, Patrick Masson
doaj   +1 more source

Dual-topology insertion of a dual-topology membrane protein. [PDF]

, 2015
Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear.
Bowie, James U, Woodall, Nicholas B, Yin, Ying   +2 more
core  

Nonenzymatic biotinylation of histone H2A [PDF]

Protein Science, 2009
AbstractHolocarboxylase synthetase (HCS, eukaryotic enzyme) and BirA (prokaryotic) are biotin protein ligases that catalyze the ATP‐dependent attachment of biotin to apocarboxylases via the reactive intermediate, bio‐5′‐AMP. In this study, we examined the in vitro mechanism of biotin attachment to histone H2A in the presence of HCS and BirA.
Shannon, Healy, Tom D, Heightman, Laura, Hohmann, David, Schriemer, Roy A, Gravel   +4 more
openaire   +2 more sources

Impact of Surface Functionalization on NV Quantum Properties: Implications for Biosensing with Fluorescent Nanodiamonds

Advanced Functional Materials, EarlyView.
Fluorescent nanodiamonds (fNDs) have emerged as an invaluable quantum sensing platform for biological and biochemical systems. This paper investigates the influence of common surface functionalization strategies for bioconjugation on the quantum properties of nitrogen vacancy (NV) centers in nanodiamonds.
Anja Sadžak, Iván Pérez, Mateja Guimarães, Lukas Spantzel, Fedor Jelezko, Michael Börsch, Anke Krueger   +6 more
wiley   +1 more source