Identification of an N-terminal glycogen synthase kinase 3 phosphorylation site which regulates the functional localisation of polycystin-2 in vivo and in vitro [PDF]
, 2006 PKD2 is mutated in 15% of patients with autosomal dominant polycystic kidney disease (ADPKD). Polycystin-2 (PC2), the PKD2 protein, is a nonselective Ca2 + -permeable cation channel which may function at the cell surface and ER. Nevertheless, the factors
Kane, M.E. +4 more
core +2 more sources
ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells
bioRxiv, 2021 Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E.
Xiyan Zhao +9 more
semanticscholar +1 more source
ABCC6 is a basolateral plasma membrane protein [PDF]
, 2013 RATIONALE:: ABCC6 plays a crucial role in ectopic calcification; mutations of the gene cause pseudoxanthoma elasticum and general arterial calcification of infancy.
Apana, A. +11 more
core +3 more sources
Biotin attenuates heat shock factor 4b transcriptional activity by lysine 444 biotinylation
Biochemistry and Biophysics Reports, 2022 Genetic mutations in HSF4 cause congenital cataracts. HSF4 exhibits both positive and negative regulation on the transcription of heat shock and non-heat shock proteins during lens development, and its activity is regulated by posttranslational ...
Longjun Yan +18 more
doaj +1 more source
Cell-Type-Specific Proteomics: A Neuroscience Perspective
Proteomes, 2018 Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and ...
Rashaun S. Wilson, Angus C. Nairn
doaj +1 more source
Mapping axon initial segment structure and function by multiplexed proximity biotinylation
Nature Communications, 2020 Axon initial segments (AISs) generate action potentials and regulate the polarized distribution of proteins, lipids, and organelles in neurons. While the mechanisms of AIS Na+ and K+ channel clustering are understood, the molecular mechanisms that ...
Hamdan Hamdan +11 more
semanticscholar +1 more source
Comparison between a direct-flow SPR immunosensor for ampicillin and a competitive conventional amperometric device: analytical features and possible applications to real samples [PDF]
, 2017 In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic.
Campanella, Luigi +6 more
core +2 more sources
Reduced neurosteroid potentiation of GABAA receptors in epilepsy and depolarized hippocampal neurons [PDF]
, 2020 OBJECTIVE: Neurosteroids regulate neuronal excitability by potentiating γ-aminobutyric acid type-A receptors (GABARs). In animal models of temporal lobe epilepsy, the neurosteroid sensitivity of GABARs is diminished and GABAR subunit composition is ...
Jansen, Laura A +3 more
core +2 more sources
STAR Protocols, 2022 Summary: Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset
Serife Ayaz-Guner +10 more
doaj +1 more source
Identification of PLXDC1 and PLXDC2 as the transmembrane receptors for the multifunctional factor PEDF. [PDF]
, 2014 Pigment Epithelium Derived Factor (PEDF) is a secreted factor that has broad biological activities. It was first identified as a neurotrophic factor and later as the most potent natural antiangiogenic factor, a stem cell niche factor, and an inhibitor of
Al-Ubaidi, Muayyad +7 more
core +1 more source