Results 61 to 70 of about 54,144 (311)
Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]
, 2013 The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore +54 more
core +1 more source
Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β. [PDF]
, 2016 Mutations in human CLC-1 chloride channel are associated with the skeletal muscle disorder myotonia congenita. The disease-causing mutant A531V manifests enhanced proteasomal degradation of CLC-1.
Chen, Shu-Ching +7 more
core +1 more source
Nonenzymatic biotinylation of histone H2A [PDF]
Protein Science, 2009 AbstractHolocarboxylase synthetase (HCS, eukaryotic enzyme) and BirA (prokaryotic) are biotin protein ligases that catalyze the ATP‐dependent attachment of biotin to apocarboxylases via the reactive intermediate, bio‐5′‐AMP. In this study, we examined the in vitro mechanism of biotin attachment to histone H2A in the presence of HCS and BirA.
Shannon, Healy +4 more
openaire +2 more sources
Oxysterol-Binding Protein-1 (OSBP1) Modulates Processing and Trafficking of the Amyloid Precursor Protein [PDF]
, 2008 BACKGROUND Evidence from biochemical, epidemiological and genetic findings indicates that cholesterol levels are linked to amyloid-β (Aβ) production and Alzheimer's disease (AD). Oxysterols, which are cholesterol-derived ligands of the liver X receptors
Abraham, Carmela R. +8 more
core +4 more sources
Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis [PDF]
, 2020 The homing of Endothelial Progenitor Cells (EPCs) to tumor angiogenic sites has been described as a multistep process, involving adhesion, migration, incorporation and sprouting, for which the underlying molecular and cellular mechanisms are yet to be ...
Bieback, Karen +6 more
core +1 more source
Sensors, 2018 We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen +5 more
doaj +1 more source
Coming together to define membrane contact sites. [PDF]
, 2019 Close proximities between organelles have been described for decades. However, only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell ...
De Matteis, Maria Antonietta +14 more
core +7 more sources
Journal of Experimental Life Science, 2019 Protein has been known as an important macromolecule which has a vital role among the living organism. One of the most interesting protein is Arc1p, which is a yeast-specific tRNA-binding protein.
Restu Nugraha +2 more
doaj +1 more source
Biotinylated Cyclooligosaccharides for Paclitaxel Solubilization [PDF]
Molecules, 2018 The poor water solubility of paclitaxel causes significant problems in producing cancer therapeutic formulations. Here, we aimed to solubilize paclitaxel using biocompatible cyclic carbohydrates. Generally recognized as safe, labeled β-cyclodextrin (β-CD), a cyclic α-1,4-glucan consisting of seven glucoses, was prepared, and bio-sourced ...
Cho, Eunae, Jung, Seunho
openaire +2 more sources
A Peptide Core Motif for Binding to Heterotrimeric G Protein α Subunits [PDF]
, 2005 Recently, in vitro selection using mRNA display was used to identify a novel peptide sequence that binds with high affinity to G{alpha}i1. The peptide was minimized to a 9-residue sequence (R6A-1) that retains high affinity and specificity for the GDP ...
Adhikari, Anirban +4 more
core +2 more sources