Results 71 to 80 of about 54,144 (311)

Site directed biotinylation of filamentous phage structural proteins

Virology Journal, 2011
Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E.
Smelyanski Larisa, Gershoni Jonathan M
doaj   +1 more source

Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR

Life, 2022
In vivo biotinylation using wild-type and mutants of biotin ligases is now widely applied for the study of cellular proteomes. The commercial availability of kits for the highly efficient purification of biotinylated proteins and their excellent ...
Darkhan Kanayev, Diana Abilmazhenova, Ilyas Akhmetollayev, Aliya Sekenova, Vyacheslav Ogay, Arman Kulyyassov   +5 more
doaj   +1 more source

Profiling the interactome of oligonucleotide drugs by proximity biotinylation

Nature Chemical Biology
Drug-ID is a novel method applying proximity biotinylation to identify drug–protein interactions inside living cells. The covalent conjugation of a drug with a biotin ligase enables targeted biotinylation and identification of the drug-bound proteome. We
Alfred Hanswillemenke, D. T. Hofacker, M. Sorgenfrei, Carolin Fruhner, M. Franz‐Wachtel, Dirk Schwarzer, Boris Maček, Thorsten Stafforst   +7 more
semanticscholar   +1 more source

Structure of the full-length TRPV2 channel by cryo-EM. [PDF]

, 2016
Transient receptor potential (TRP) proteins form a superfamily Ca(2+)-permeable cation channels regulated by a range of chemical and physical stimuli. Structural analysis of a 'minimal' TRP vanilloid subtype 1 (TRPV1) elucidated a mechanism of channel ...
Cohen, Matthew R, Huynh, Kevin W, Jiang, Jiansen, Lodowski, David T, Moiseenkova-Bell, Vera Y, Samanta, Amrita, Zhou, Z Hong   +6 more
core   +2 more sources

Proximity Utilizing Biotinylation of Nuclear Proteins in vivo

Central Asian Journal of Global Health, 2015
Introduction. The human genome consists of roughly 30,000 genes coding for over 500,000 different proteins, of which more than 10,000 proteins can be produced by the cell at any given time (the cellular “proteome”). It has been estimated that over 80% of
Arman Kulyyassov, Gulsamal Zhubanova, Erlan Ramanculov, Vasily Ogryzko   +3 more
doaj   +1 more source

Force dependent biotinylation of myosin IIA by α-catenin tagged with a promiscuous biotin ligase. [PDF]

PLoS ONE, 2015
Tissues and organs undergo constant physical perturbations and individual cells must respond to mechanical forces to maintain tissue integrity. However, molecular interactions underlying mechano-transduction are not fully defined at cell-cell junctions ...
Shuji Ueda, Alexandra M Blee, Katherine G Macway, Derrick J Renner, Soichiro Yamada   +4 more
doaj   +1 more source

Automated Solid-Phase Protein Modification with Integrated Enzymatic Digest for Reaction Validation: Application of a Compartmented Microfluidic Reactor for Rapid Optimization and Analysis of Protein Biotinylation

Frontiers in Bioengineering and Biotechnology, 2017
Protein modification by covalent coupling of small ligands or markers is an important prerequisite for the use of proteins in many applications. Well-known examples are the use of proteins with fluorescent markers in many in vivo experiments or the ...
Regina Fraas, Juliane Diehm, Matthias Franzreb   +2 more
doaj   +1 more source

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures [PDF]

, 2019
The fidelity of inhibitory neurotransmission is dependent on the accumulation of γ-aminobutyric acid type A receptors (GABAARs) at the appropriate synaptic sites.
Andrews, Peter M., Brandon, Nicholas J., Davies, Paul A., Hines, Rochelle M., Moore, Yvonne E., Moss, Stephen J., Nathanson, Anna J., Ollerhead, Thomas A., Rodriguez Santos, Miguel A., Smalley, Joshua L., Wobst, Heike J., Zhang, Yihui   +11 more
core   +2 more sources

Tissue Specific Labeling in Proteomics

Proteomes, 2017
Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in ...
Evelyn Ramberger, Gunnar Dittmar
doaj   +1 more source

Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants

Bio-Protocol, 2022
Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin.
Nicole Gibbs, Shih- Su, Patrick Masson
doaj   +1 more source