Results 71 to 80 of about 49,644 (261)

Revealing and mitigating the inhibitory effect of serotonin on HRP-mediated protein labelling

Scientific Reports
Proximity-dependent biotinylation coupled with mass spectrometry enables the characterization of subcellular proteomes. This technique has significantly advanced neuroscience by revealing sub-synaptic protein networks, such as the synaptic cleft and post-
Zora Chui-Kuen Chan, Cheng Qi, Yuanhong Cai, Xin Li, Jing Ren   +4 more
doaj   +1 more source

A General Method to Screen Nanobodies for Cytochrome P450 Enzymes from a Yeast Surface Display Library

Biomedicines
The availability of yeast surface display nanobody (Nb) libraries offers a convenient way to acquire antigen-specific nanobodies that may be useful for protein structure–function studies and/or therapeutic applications, complementary to the conventional ...
Yudong Sun, Cristian Martinez-Ramos, Eugene Chen, Yoichi Osawa, Haoming Zhang   +4 more
doaj   +1 more source

Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking

BioTechniques, 2011
We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which ...
Faraz Ahmad, Sarah Kate Coleman, Kai Kaila, Peter Blaesse   +3 more
doaj   +1 more source

Profound Re-Organization of Cell Surface Proteome in Equine Retinal Pigment Epithelial Cells in Response to In Vitro Culturing [PDF]

, 2012
The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. The RPE plays an essential role in visual function and represents the outer blood-retinal barrier.
Aisenbrey, Alcazar, Alge, Bhutto, Bodaghi, Burke, Campochiaro, Christoph Szober, Chu, Claudia Alge-Priglinger, Clegg, Cornelia Deeg, Deeg, Deeg, Deeg, Deeg, Deeg, Degroote, Doherty, Dunn, Eberhardt, Ehrenhofer, Greer, Gullapalli, Gundersen, Ham, Hamel, Hauck, Hauck, Hauck, Hauck, Ho, Huang, Karl, Kerstin Euler, Kristina Fröhlich, Li, Limb, Machemer, Marius Ueffing, McBee, Milyushina, Mochizuki, Muramatsu, Nagai, Parker, Philp, Rizzolo, Rotilio, Singer, Stefanie Hauck, Strauss, Vacanti, Wolf, Wu   +54 more
core   +3 more sources

Biological functions of biotinylated histones [PDF]

The Journal of Nutritional Biochemistry, 2005
Histones H1, H2A, H2B, H3 and H4 are DNA-binding proteins that mediate the folding of DNA into chromatin. Various posttranslational modifications of histones regulate processes such as transcription, replication and repair of DNA. Recently, a novel posttranslational modification has been identified: covalent binding of the vitamin biotin to lysine ...
Nagarama, Kothapalli, Gabriela, Camporeale, Alice, Kueh, Yap C, Chew, Anna M, Oommen, Jacob B, Griffin, Janos, Zempleni   +6 more
openaire   +2 more sources

Balanced Expression of the Diiron Oxygenase BioE Is Essential for Biotin Homeostasis in Elizabethkingia meningoseptica

Advanced Science, EarlyView.
BioE is a new diiron oxygenase that catalyzes the conversion of long‐chain acyl groups into pimeloyl thioester, initiating biotin synthesis. The overexpression of EmBioE disrupts lipid metabolic homeostasis, requiring repressor BioL to maintain a balance between long‐chain fatty acids and biotin synthesis.
Meng Zhang, Ying‐ying Fu, Xiaoqiang Yang, Qiuying Qin, Xinyu Su, Jiaming Fang, Yanhua Kang, Qingwen He, Zhi Ruan, Yongchang Xu   +9 more
wiley   +1 more source

High-Throughput Biotinylation of Proteins [PDF]

, 2009
One of the more useful tags for a protein in biochemical experiments is biotin, because of its femtomolar dissociation constant with streptavidin or avidin. Robust methodologies have been developed for other the in vivo addition of a single biotin to recombinant protein or the in vitro enzymatic or chemical addition of biotin to a protein.
Brian K, Kay, Sang, Thai, Veronica V, Volgina   +2 more
openaire   +2 more sources

Development of Endogenous Protein Probes for Characterizing Surface Proteins and Cellular Interactors of Extracellular Vesicles

Advanced Science, EarlyView.
The proximity labeling enzyme APEX2 is displayed on extracellular vesicle (EV) surfaces via genetic fusion with EV‐sorting scaffolds, enabling in situ biotinylation of native surface proteins, adsorbed corona components, and interacting cellular proteins.
Wenyi Zheng, Metoboroghene Mowoe, Wenqing Hou, Daniel W. Hagey, Koshi Imami, Samir EL Andaloussi   +5 more
wiley   +1 more source

Palmitoylation‐Mediated Ubiquitination of SRPK1 Regulates Ferroptosis in High‐Fat‐Induced Erectile Dysfunction

Advanced Science, EarlyView.
Elevated exogenous palmitic acid promotes the S‐palmitoylation of SRPK1 in endothelial cells, a dynamic process governed by ZDHHC24 and APT1. This post‐translational modification strengthens the interaction between SRPK1 and the E3 ubiquitin ligase MIB1, thereby facilitating the proteasomal degradation of SRPK1.
Xiao‐Hui Tan, Ke‐Fan Li, Yi‐Ming Yuan, Man‐Cheng Xia, Fang‐Zhou Zhao, Hong‐Gang Ying, Zhuo Zhou, Peng‐Chao Gao, Guo‐Qing Xie, Xue‐Song Li, Hui Jiang, Rui‐Li Guan   +11 more
wiley   +1 more source

Proteomic Analysis of Regulated Dendritic Cell Endosomes Reveals Dynamic Adaptation to Antigen Uptake and Cross‐Presentation [PDF]

Eur J Immunol
Phagocytosis of yeast activates recruitment of proteases and trafficking factors from the secretory and endosomal pathways to the IRAP compartment and subsequently phagosomes, promoting cross‐presentation. In the absence of Sec22b, IRAP endosome and phagosome maturation to late endosomes is accelerated.
Senni A, Grumbach L, Ceccacci S, Frechin M, Goudin N, Guerrera I, Bertocci B, van Endert P.   +7 more
europepmc   +2 more sources