Results 81 to 90 of about 54,144 (311)
PLoS ONE, 2020 Toxoplasma gondii is an obligate intracellular parasite which is capable of establishing life-long chronic infection in any mammalian host. During the intracellular life cycle, the parasite secretes an array of proteins into the parasitophorous vacuole ...
S. Nadipuram +4 more
semanticscholar +1 more source
High-Throughput Biotinylation of Proteins [PDF]
, 2009 One of the more useful tags for a protein in biochemical experiments is biotin, because of its femtomolar dissociation constant with streptavidin or avidin. Robust methodologies have been developed for other the in vivo addition of a single biotin to recombinant protein or the in vitro enzymatic or chemical addition of biotin to a protein.
Brian K, Kay +2 more
openaire +2 more sources
Biological functions of biotinylated histones [PDF]
The Journal of Nutritional Biochemistry, 2005 Histones H1, H2A, H2B, H3 and H4 are DNA-binding proteins that mediate the folding of DNA into chromatin. Various posttranslational modifications of histones regulate processes such as transcription, replication and repair of DNA. Recently, a novel posttranslational modification has been identified: covalent binding of the vitamin biotin to lysine ...
Nagarama, Kothapalli +6 more
openaire +2 more sources
Advanced Functional Materials, EarlyView.Fluorescent nanodiamonds (fNDs) have emerged as an invaluable quantum sensing platform for biological and biochemical systems. This paper investigates the influence of common surface functionalization strategies for bioconjugation on the quantum properties of nitrogen vacancy (NV) centers in nanodiamonds.
Anja Sadžak +6 more
wiley +1 more source
Chlamydia suis, an emerging Chlamydiaceae species in pigs? [PDF]
, 2010 Chlamydiaceae are Gram-negative obligate intracellular bacteria that can infect a broad range of animals and humans. Chlamydial infection of livestock, companion animals, and other animals may result in conjunctivitis, enteritis, pneumonia, abortion ...
Beeckman, Delphine Sylvie Anne +7 more
core +1 more source
Profound Re-Organization of Cell Surface Proteome in Equine Retinal Pigment Epithelial Cells in Response to In Vitro Culturing [PDF]
, 2012 The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. The RPE plays an essential role in visual function and represents the outer blood-retinal barrier.
Aisenbrey +54 more
core +3 more sources
Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation
Journal of Cell Biology, 2020 Chastney et al. use multiplexed proximity-dependent labeling to define the topology of integrin adhesion complexes. The data support current models of adhesome architecture and provide a resource to examine proximal relationships within the adhesome and ...
Megan R. Chastney +7 more
semanticscholar +1 more source
Advanced Materials, EarlyView.Detecting proteins secreted by a single cell while retaining its viability remains challenging. A particles‐in‐particle (PiPs) system made by co‐encapsulating barcoded microparticles (BMPs) with a single cell inside an alginate hydrogel particle is introduced.
Félix Lussier +10 more
wiley +1 more source
Small-molecule CaVα1⋅CaVβ antagonist suppresses neuronal voltage-gated calcium-channel trafficking [PDF]
, 2018 Extracellular calcium flow through neuronal voltage-gated CaV2.2 calcium channels converts action potential-encoded information to the release of pronociceptive neurotransmitters in the dorsal horn of the spinal cord, culminating in excitation of the ...
Chen, Xingjuan +10 more
core +1 more source
Biotinylation as a tool to enhance the uptake of small molecules in Gram-negative bacteria.
PLoS ONE, 2021 Antibiotic resistance is a major public health concern. The shrinking selection of effective antibiotics and lack of new development is making the situation worse.
Ankit Pandeya +6 more
doaj +2 more sources