Results 81 to 90 of about 33,953 (257)

Precisely biotinylated RNA [PDF]

Nature Methods, 2005
Using unnatural base pairs, researchers have site-specifically biotinylated RNA, allowing them to immobilize an aptamer on a chip and detect its interaction with a target protein.
openaire   +2 more sources

Biotinylation of Membrane Proteins for Binder Selections [PDF]

, 2020
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the experimental outcome. This poses specific challenges for membrane proteins due to their inherent lack of
Kuhn, Benedikt T, Zimmermann, Iwan, Egloff, Pascal, Hürlimann, Lea M, Hutter, Cedric A J, Miscenic, Christian, Dawson, Roger J P, Seeger, Markus A, Geertsma, Eric R   +8 more
openaire   +3 more sources

On‐Paper Enzyme‐Linked and Metal‐Linked Immunosorbent Assays for Low‐Cost Decentralized Testing of Carcinoembryonic Antigen

Analysis &Sensing, EarlyView.
On‐paper enzyme‐linked and metal‐linked immunosorbent assays (p‐ELISA and p‐MeLISA, respectively) were developed for the assay of carcinoembryonic antigen (CEA) in serum. These approaches are based on bio‐conjugated paper disks as analytical platform.
Electra Mermiga, Varvara Pagkali, Dionysios Soulis, Christos Kokkinos, Anastasios Economou   +4 more
wiley   +1 more source

The Importance of Biotinylation for the Suitability of Cationic and Neutral Fourth-Generation Polyamidoamine Dendrimers as Targeted Drug Carriers in the Therapy of Glioma and Liver Cancer

Molecules
In this study, we hypothesized that biotinylated and/or glycidol-flanked fourth-generation polyamidoamine (PAMAM G4) dendrimers could be a tool for efficient drug transport into glioma and liver cancer cells.
Łukasz Uram, Magdalena Twardowska, Żaneta Szymaszek, Maria Misiorek, Andrzej Łyskowski, Zuzanna Setkowicz, Zuzanna Rauk, Stanisław Wołowiec   +7 more
doaj   +1 more source

Photoactivatable Alkyne Tag for Photolabeling Biomolecules in Living Cells

ChemBioChem, EarlyView.
A photoactivatable linear alkyne tag is developed by caging the intermediate of the Eschenmoser–Tanabe reaction to expand photoinduced intracellular molecular imaging to a variety of biomolecules. The photoactivatable tag is modified onto a cholesterol analog and introduced into cells. After light irradiation, the tag is successfully labeled with azide‐
Yuki Umeda, Hao Zhu, Satoshi Yamaguchi, Sho Nakamura, Masato Takada, Shin Izuta, Akimitsu Okamoto   +6 more
wiley   +1 more source

Quantitative analysis of SR-BI-dependent HDL retroendocytosis in hepatocytes and fibroblasts

Journal of Lipid Research, 2006
Previous studies have suggested that HDL retroendocytosis may play a role in scavenger receptor class B type I (SR-BI)-dependent selective lipid uptake in a cell-specific manner.
Bing Sun, Erik R.M. Eckhardt, Shoba Shetty, Deneys R. van der Westhuyzen, Nancy R. Webb   +4 more
doaj  

Signal-On Detection of Caspase-3 with Methylene Blue-Loaded Metal-Organic Frameworks as Signal Reporters

Molecules
In this work, we report on an electrochemical method for the signal-on detection of caspase-3 and the evaluation of apoptosis based on the biotinylation reaction and the signal amplification of methylene blue (MB)-loaded metal–organic frameworks (MOFs ...
Yaliang Huang, Jiaqiang Wang, Yirui Xu, Jiwen Zhang, Ning Xia   +4 more
doaj   +1 more source

Emerging Target Discovery Strategies Drive the Decoding of Therapeutic Power of Natural Products and Further Drug Development: A Case Study of Celastrol

Exploration, EarlyView.
Using celastrol as a case study, this review summarizes various target discovery strategies for natural products, including chemical proteomics, protein microarray, degradation‐based protein profiling, proteome‐wide label‐free approaches, network pharmacology, target‐based drug screening, and indirect strategies.
Yanbei Tu, Guiyu Dai, Yanyan Chen, Lihua Tan, Hanqing Liu, Meiwan Chen   +5 more
wiley   +1 more source

Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides

Journal of Analytical Methods in Chemistry, 2016
Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5 ...
Chun Wu
doaj   +1 more source

Decoding RNA–Protein Interactions: Methodological Advances and Emerging Challenges

Advanced Genetics, Volume 6, Issue 2, June 2025.
This review provides a comprehensive comparison of RNA‐ and protein‐centric methodologies for investigating RNA–protein interactions, emphasizing their respective advantages, limitations, and suitable applications. It highlights recent technological innovations, outlines strategic method selection criteria, and addresses key challenges.
Wenkai Yi, Jian Yan
wiley   +1 more source