Results 111 to 120 of about 1,442,988 (353)

A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N, a new regenerated cellulose virus removal filter

Biotechnology Progress, EarlyView.
Abstract Virus removal by filtration is a crucial step in ensuring the safety of therapeutic antibodies and other biopharmaceutical products by mitigating the risk of endogenous and adventitious viral contamination. However, there are monoclonal antibodies (mAb) that are difficult to filter effectively using virus removal filters (i.e., challenging ...
Asami Inoue, Satoshi Murakami, Akika Futamura, Naoto Watanabe, Yumiko Masuda   +4 more
wiley   +1 more source

Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies

STAR Protocols, 2022
Summary: Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources.
Danqing Li, Alexander C. Partin, Liangjun Zhao, Irwin Chen, Mark L. Michaels, Zhulun Wang, Fernando Garces, Danyang Gong, Timothy P. Riley   +8 more
doaj  

[Bispecific antibodies: what future?].

Medecine sciences : M/S, 2009
Monoclonal antibodies have emerged as a very successful class of therapeutic agents. In their native format, monoclonal antibodies are monospecific in that they recognize only one epitope, but their Fc domain also binds to FcfR-expressing cells. Attempts to improve the cytotoxicity of antibodies, particularly in the cancer field, have led to the design
Pèlegrin, André, Robert, Bruno
openaire   +3 more sources

Improving outcomes in intensified processing via optimization of the cell line development workflow

Biotechnology Progress, EarlyView.
Abstract As the industry continues to explore the benefits of continuous and intensified manufacturing, it is important to assure that the cell line development (CLD) workflows in practice today are well suited to generate clones that meet the unique challenges associated with these processes. Most cell lines used in intensified processes are currently
Vincent Balassi, Mary Otto, Corey Kretzmer, Amber Petersen, Channing McLaurin, Jana Mahadevan, Jason Gustin, Trissa Borgschulte, David Razafsky   +8 more
wiley   +1 more source

Techno‐economic analysis of membrane‐based continuous capture chromatography platforms for large‐scale antibody production

Biotechnology Progress, EarlyView.
Abstract Continuous manufacturing platforms and membrane chromatography are process technologies with the potential to reduce production costs and minimize process variability in monoclonal antibody production. This study presents a simulation and optimization framework to perform techno‐economic analyses of these strategies.
Juan J. Romero, Eleanor W. Jenkins, Marc R. Birtwistle, Scott M. Husson   +3 more
wiley   +1 more source

Structural and functional characterization of IgG- and non-IgG-based T-cell-engaging bispecific antibodies

Frontiers in Immunology
Bispecific T-cell-engaging antibodies are a growing class of therapeutics with numerous molecules being tested in clinical trials and, currently, seven of them have received market approval.
Nishant Mohan, Safiat Ayinde, Hanjing Peng, Shraboni Dutta, Yi Shen, Vincent M. Falkowski, Thomas G. Biel, Tongzhong Ju, Wen Jin Wu   +8 more
doaj   +1 more source

Function and mechanism of bispecific antibodies targeting SARS-CoV-2

Cell Insight
As the dynamic evolution of SARS-CoV-2 led to reduced efficacy in monoclonal neutralizing antibodies and emergence of immune escape, the role of bispecific antibodies becomes crucial in bolstering antiviral activity and suppressing immune evasion.
Zhaohui Li, Zengyuan Zhang, Steven T. Rosen, Mingye Feng   +3 more
doaj  

Bp-Bs, a Novel T-cell Engaging Bispecific Antibody with Biparatopic Her2 Binding, Has Potent Anti-tumor Activities

Molecular Therapy: Oncolytics, 2019
Patients with Human epidermal growth factor receptor type 2 (Her2) overexpression are associated with aggressive tumor growth and poor clinical outcomes.
Jiayu Liu, Xiaoqiong Wu, Limin Lin, Haitao Pan, Yanlan Wang, Yumei Li, Yining Zhao, Zhong Wang   +7 more
doaj  

The Molecular Toolbox for Linkage Type‐Specific Analysis of Ubiquitin Signaling

ChemBioChem, EarlyView.
Ubiquitin modifications regulate virtually all aspects of eukaryotic cell biology through an array of polyubiquitin chain signals that adopt distinct structures, enabling the individual polyubiquitin linkages to mediate specific functions in cells.
Julian Koch, Camilla Reiter Elbæk, Dominik Priesmann, Rune Busk Damgaard   +3 more
wiley   +1 more source

Methods for the Generation of Single‐Payload Antibody‐Drug Conjugates

ChemMedChem, EarlyView.
Antibody‐drug conjugates (ADCs) have emerged as a powerful form of targeted therapeutic. However, despite the myriad methods to generate even‐integer drug‐to‐antibody ratio (DAR) ADCs, strategies to generate DAR 1 are seldom reported despite the property tuning advantages of low drug loading or in enabling access to antibody‐protein constructs.
Thomas Wharton, David R. Spring
wiley   +1 more source