Results 141 to 150 of about 67,281 (289)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
The role of conflict & negotiation in the complexity of projects
, 2012 Projects are pervasive and disparate spanning a plethora of domains. Most projects are unified by certain characteristics regardless of the sector or industry to which they belong i.e.
Gul, S
core
Deriving metrics for relating complexity measures to software maintenance costs [PDF]
, 1982 Managers of software maintenance functions know that maintenance costs often increase with the age of software, and that maintenance costs are frequently proportional to software complexity. When the service to expense ratio degrades, sometimes a rewrite
Taylor, J., Connell, J., Brice, L.
core
FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
, 2002 The author proposes a block cipher wich is easy to implement in software on modern 32 bit microprocessorsThe building blocks of the cipher are from the block ciphers MMB and SAFER. The cipher may be expanded for use with future 64 bit processors.
Dieter Schmidt
core
Homocoupling of C 1 building blocks by a heterometallic Mg–Al complex
Dalton TransactionsA heterometallic magnesium–aluminium hydride complex is reported for the selective homologation of C 1 building blocks (CO and CNXyl, Xyl = 2,6-Me 2 C 6 H 3 ) to form ...
Wenbang Yang, Andrew White, Mark Crimmin
openaire +2 more sources
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
, 2007 The present analysis investigates whether and to what degree quantifiable spatial attributes, as expressed in plan representations, can capture elements related to the experience of spatial identity.
Laskari, A.
core
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source