Results 121 to 130 of about 2,362,330 (266)

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture [PDF]

, 2016
Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic.
Ellis, MJ, Luetchford, KA, Regan, S, Sathish, J, Shipley, R, Sorrell, I, Storm, MP, Webb, SD   +7 more
core   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Melioidosis, Northeastern Brazil [PDF]

, 2005
Melioidosis is a fatal bacterial infection found in many parts of the tropical belt, particularly in Southeast Asia and northern Australia. Sporadic cases of the disease have been reported previously in Central and South America.
Rolim, D.B., Vilar, D.C.F.L., Sousa, A.Q., Miralles, I.S., de Oliveira, D.C.A., Harnett, G., O'Reilly, L., Howard, K., Sampson, I., Inglis, T.J.J.   +9 more
core   +2 more sources

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

Identificación directa de microorganismos a partir de muestras de orina y hemocultivos utilizando MALDI-TOF

Infectio, 2019
Objective: To evaluate the utility of the direct identification of microorganisms in urine and blood cultures samples, using MALDI-TOF by evaluating concordance for identification, time to obtain an identification result and associated costs. Materials
Elizabeth Sierra
doaj   +1 more source

Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte [PDF]

, 2008
The H37Rv strain of MTB was grown in modified Sauton’s medium till mid-log phase. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient sedimentation on Ficoll-Paque separation medium.
Ajay Kumar, Jawed Iqbal, Najmul Islam
core   +2 more sources

Targeting TNBC: core–shell polycationic polyurea dendrimers with inherent anticancer activity

FEBS Open Bio, EarlyView.
Core–shell polycationic PURE dendrimers were tested in TNBC‐derived tumor models. Both formulations selectively targeted TNBC and effectively reduced tumor volume. PUREG4‐OEI48 suppressed tumor growth without detectable toxicity, whereas PUREG4‐OCEI24, despite showing efficacy, induced hepatic toxicity.
Adriana Cruz, Bruna Abreu, Cindy Mendes, Catarina Freitas‐Dias, Filipe Gonçalves, Fernanda Silva, José Ramalho, Saudade André, Vasco D. B. Bonifácio, Jacinta Serpa   +9 more
wiley   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Microbiological profile and antimicrobial resistance patterns of bloodstream infections in a tertiary care hospital in eastern India

Current Medicine Research and Practice
Background: Bloodstream infections (BSIs) remain a significant cause of global morbidity and mortality, compounded by the escalating public health crisis of antimicrobial resistance (AMR). Aims: This study aimed to analyse the demographic characteristics,
Sangeeta Datta, Avinash Kumar, Biswaroop Chatterjee   +2 more
doaj   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source