Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
Electrophoresis 30, S1 (2009) S174-S180, 2009 Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with different proteins, while most protein chemistry methods work mainly, if not only, in water-based ...
arxiv +1 more source
Characterisation of nicotine receptors on human peripheral blood mononuclear cells (PBMC) [PDF]
, 2009 “The original publication is available at www.springerlink.com”. Copyright Springer. DOI: 10.1007/s00011-008-8171-xAim and objective: The aim of the work was to characterise the nAChRs on human PBMC.
Parsons, M. +2 more
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Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis [PDF]
Methods in molecular biology (Clifton, N.J.) 528 (2009) 259-67, 2009 Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules.
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Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon [PDF]
Avicenna Journal of Medical Biochemistry, 2018 Background: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy ...
Judith Gwladys Ekwe Priso +7 more
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Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]
Methods in molecular biology (Clifton, N.J.) 432 (2008) 83-100, 2008 Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial proteins with two-dimensional electrophoresis (2DE), showing some adjustment over the classical ...
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Effects of anabolic steroids on acute phase responses in intra-abdominal sepsis
Mediators of Inflammation, 1997 The acute phase response is an important adaptive response to sepsis and injury. As anabolic steroids increase protein synthesis we postulated that these agents might also increase hepatic acute phase protein synthesis.
K. Mealy +4 more
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Two-dimensional SDS-PAGE fractionation of biological samples for biomarker discovery [PDF]
Methods in Molecular Biology -Clifton then Totowa- 1002 (2013)
151-65, 2013 Two-dimensional electrophoresis is still a very valuable tool in proteomics, due to its reproducibility and its ability to analyze complete proteins. However, due to its sensitivity to dynamic range issues, its most suitable use in the frame of biomarker discovery is not on very complex fluids such as plasma, but rather on more proximal, simpler fluids
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Semina: Ciências Agrárias, 2014 There are several diseases that can affect pets. Recognize the infirm at risk of death would allow early medical interventions and the establishment of different monitoring protocols, increasing survival.
Andrei Kelliton Fabretti +5 more
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Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]
, 1993 A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman +29 more
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Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
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