Results 51 to 60 of about 1,056,334 (396)

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

Journal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
arxiv   +1 more source

Serum Protein Gel Agarose Electrophoresis in Captive Tigers

Animals, 2020
Given the endangered status of tigers (Panthera tigris), the health of each individual is important and any data on blood chemistry values can provide valuable information alongside the assessment of physical condition.
Daniela Proverbio, Roberta Perego, Luciana Baggiani, Giuliano Ravasio, Daniela Giambellini, Eva Spada   +5 more
doaj   +1 more source

An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]

IEEE Electro/Information Technology, 2007, 2016
Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv   +1 more source

Machine learning evaluation for identification of M-proteins in human serum.

PLoS ONE
Serum electrophoresis (SPEP) is a method used to analyze the distribution of the most important proteins in the blood. The major clinical question is the presence of monoclonal fraction(s) of antibodies (M-protein/paraprotein), which is essential for the
Alexandros Sopasakis, Maria Nilsson, Mattias Askenmo, Fredrik Nyholm, Lillemor Mattsson Hultén, Victoria Rotter Sopasakis   +5 more
doaj   +1 more source

Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes [PDF]

Journal of Proteomics (2012) epub ahead of print, 2012
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear proteins lies in their strong interaction with nuclear acids. Several experimental procedures have been tested to increase, in the nuclear extract, the ratio of nuclear ...
arxiv   +1 more source

High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia

Journal of Translational Medicine, 2009
Background When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens.
Thiel Eckhard, Sinha Pranav, Poland Julia, Nowak Daniel, Letsch Anne, Hütter Gero, Hofmann Wolf-K   +6 more
doaj   +1 more source

A protein corona-enabled blood test for early cancer detection.

Nanoscale, 2017
Pancreatic cancer is a very aggressive malignancy that is often diagnosed in the advanced stages, with the implication that long-term survivors are extremely rare.
D. Caputo, Massimiliano Papi, Roberto Coppola, S. Palchetti, L. Digiacomo, G. Caracciolo, D. Pozzi   +6 more
semanticscholar   +1 more source

Selection Of A Novel Aptamer Against Vitronectin Using Capillary Electrophoresis And Next Generation Sequencing [PDF]

, 2016
Breast cancer (BC) results in ≃40,000 deaths each year in the United States and even among survivors treatment of the disease may have devastating consequences, including increased risk for heart disease and cognitive impairment resulting from the toxic ...
Boyacioglu, O., Colyer, C. L., Debinski, W., Gmeiner, W. H., Herpai, D. M., Marini, F. C., Qasem, S., Riley, Kathryn R., Stuart, C. H.   +8 more
core   +3 more sources

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

Molecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever, Renske D. M. Steenbergen   +1 more
wiley   +1 more source

Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view [PDF]

Electrophoresis 27 (04/2006) 1574-83, 2006
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial rRNAs and a few tRNAs, we performed a comparative study on the 143B cell line and on its Rho-0 ...
arxiv   +1 more source