Results 11 to 20 of about 575,594 (234)
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
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Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
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Measuring protein levels in planarians using western blotting
STAR Protocols, 2021 Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
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Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Iranian Journal of Public Health, 2005 Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz, AJ Dickson
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Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
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Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
Bio-Protocol, 2023 The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research.
Jingjing Zang, Stephan Neuhauss
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Western Blotting for Staphylococcus aureus AgrA
Bio-Protocol, 2014 Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII.
Chikara Kaito, Kazuhisa Sekimizu
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Virus Overlay Assay (Far-Western blotting)
Bio-Protocol, 2013 Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Kun-Tong Jia +3 more
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Simple analysis of gel images with IOCBIO Gel
BMC Biology, 2023 Background Current solutions for the analysis of Western Blot images lack either transparency and reproducibility or can be tedious to use if one has to ensure the reproducibility of the analysis.
Jaak Kütt +9 more
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Detection of ASC Oligomerization by Western Blotting
Bio-Protocol, 2017 The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1.
Jérôme Lugrin, Fabio Martinon
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