Results 11 to 20 of about 548,148 (264)
Journal of Advances in VetBio Science and Techniques, 2021 Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
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Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
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Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Amy E. Herr, Alex J. Hughes
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Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
Bio-Protocol, 2023 The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research.
Jingjing Zang, Stephan Neuhauss
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Western Blotting for Staphylococcus aureus AgrA
Bio-Protocol, 2014 Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII.
Chikara Kaito, Kazuhisa Sekimizu
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Virus Overlay Assay (Far-Western blotting)
Bio-Protocol, 2013 Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Kun-Tong Jia +3 more
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Simple analysis of gel images with IOCBIO Gel
BMC Biology, 2023 Background Current solutions for the analysis of Western Blot images lack either transparency and reproducibility or can be tedious to use if one has to ensure the reproducibility of the analysis.
Jaak Kütt +9 more
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Semina: Ciências Agrárias, 2019 Objetivou-se com esse estudo avaliar a prevalência da Artrite Encefalite Caprina (CAE) em reprodutores dos estados do Maranhão, Ceará, Piauí, Alagoas, Sergipe, Rio Grande do Norte e Paraíba.
Maximiana Mesquita de Sousa +8 more
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Detection of ASC Oligomerization by Western Blotting
Bio-Protocol, 2017 The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1.
Jérôme Lugrin, Fabio Martinon
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