Results 21 to 30 of about 548,148 (264)

Western Blotting: An Introduction [PDF]

, 2015
Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979.
Biji T. Kurien, Biji T. Kurien, Biji T. Kurien, R. Hal Scofield, R. Hal Scofield, R. Hal Scofield   +5 more
openaire   +3 more sources

Single-cell western blotting [PDF]

Nature Methods, 2014
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h. A microscope slide supporting a 30-μm-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis ...
Hughes, Alex J, Spelke, Dawn P, Xu, Zhuchen, Kang, Chi-Chih, Schaffer, David V, Herr, Amy E   +5 more
openaire   +5 more sources

Evaluation of western blotting for the diagnosis of enzootic bovine leukemia

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 1999
A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally
E.T. Gonzalez, G.A. Oliva, J. Norimine, V. Cid de la Paz, M.G. Echeverría   +4 more
doaj   +1 more source

A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals

Journal of Veterinary Research, 2022
Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection.
Badr Yassien, Rahman Md. Matiur, Ohno Yoshito, Ishijima Keita, Maeda Ken, Kohyama Kaoru, Kamatari Yuji O., Shimizu Kaori, Okada Ayaka, Inoshima Yasuo   +9 more
doaj   +1 more source

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Biological Procedures Online, 2004
Three cDNAs, termed EpC1, TPxEg and EgG5, were isolated by immunoscreening from an Echinococcus granulosus cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from
Li Jun, Zhang Wen-Bao, McManus Donald P.
doaj   +1 more source

Induction of Extracellular Matrix Glycoproteins in Brassica Petioles by Wounding and in Response to Xanthomonas campestris

Molecular Plant-Microbe Interactions, 1997
A panel of monoclonal antibodies that recognize plant extracellular matrix glycoproteins previously implicated in plant-microbe interactions was used to study the effects of pathogen inoculation and wounding on glycoproteins in petioles of Brassica ...
Huw A. Davies, Michael J. Daniels, J. Maxwell Dow   +2 more
doaj   +1 more source

Prognostic Value of Immunoglobulin G (IgG) Patterns by Western Blotting Immunodetection in Treated Dogs Previously Infected with Leishmania infantum

Veterinary Sciences, 2021
Leishmaniasis is a heterogeneous group of neglected tropical diseases with various clinical syndromes, which is caused by obligate intracellular protozoa of the genus Leishmania and transmitted by the bite of a female phlebotomine sandfly.
Ehab Kotb Elmahallawy, Stefania Zanet, Marco Poggi, Khalaf F. Alsharif, Maha S. Lokman, Anna Trisciuoglio, Ezio Ferroglio   +6 more
doaj   +1 more source

Western blot

, 2022
western blotting analysis of CD63,CD81,TSG101,calnexin,CD56,perforin and ...
openaire   +1 more source

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Measuring protein levels in planarians using western blotting

STAR Protocols, 2021
Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ...
Benjamin Ziman, Néstor J. Oviedo
doaj