Results 21 to 30 of about 575,594 (234)

Estudo epidemiológico da Artrite Encefalite Caprina á vírus (CAEV) com ênfase em reprodutores de rebanhos do Nordeste do Brasil

open access: yesSemina: Ciências Agrárias, 2019
Objetivou-se com esse estudo avaliar a prevalência da Artrite Encefalite Caprina (CAE) em reprodutores dos estados do Maranhão, Ceará, Piauí, Alagoas, Sergipe, Rio Grande do Norte e Paraíba.
Maximiana Mesquita de Sousa   +8 more
doaj   +1 more source

Western blotting (immunoblotting): history, theory, uses, protocol and problems

open access: yesBioTechniques, 2023
Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues.
Rasheed Sule   +2 more
doaj   +1 more source

Evaluation of western blotting for the diagnosis of enzootic bovine leukemia

open access: yesArquivo Brasileiro de Medicina Veterinária e Zootecnia, 1999
A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally
E.T. Gonzalez   +4 more
doaj   +1 more source

Prognostic Value of Immunoglobulin G (IgG) Patterns by Western Blotting Immunodetection in Treated Dogs Previously Infected with Leishmania infantum

open access: yesVeterinary Sciences, 2021
Leishmaniasis is a heterogeneous group of neglected tropical diseases with various clinical syndromes, which is caused by obligate intracellular protozoa of the genus Leishmania and transmitted by the bite of a female phlebotomine sandfly.
Ehab Kotb Elmahallawy   +6 more
doaj   +1 more source

Induction of Extracellular Matrix Glycoproteins in Brassica Petioles by Wounding and in Response to Xanthomonas campestris

open access: yesMolecular Plant-Microbe Interactions, 1997
A panel of monoclonal antibodies that recognize plant extracellular matrix glycoproteins previously implicated in plant-microbe interactions was used to study the effects of pathogen inoculation and wounding on glycoproteins in petioles of Brassica ...
Huw A. Davies   +2 more
doaj   +1 more source

Recombinant antigens for immunodiagnosis of cystic echinococcosis

open access: yesBiological Procedures Online, 2004
Three cDNAs, termed EpC1, TPxEg and EgG5, were isolated by immunoscreening from an Echinococcus granulosus cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from
Li Jun, Zhang Wen-Bao, McManus Donald P.
doaj   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

open access: yesFEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino   +7 more
wiley   +1 more source

Western Blot diagnostic yield for simultaneous antibody detection in patients with human cysticercosis, hydatidosis, and human fascioliasis

open access: yesRevista Peruana de Medicina Experimental y Salud Pública, 2016
Objectives. To determine the diagnostic yield using western blotting to simultaneously detect antibodies in patients with human cysticercosis, hydatidosis, and human fascioliasis. Materials and methods.
Kelly Davelois   +2 more
doaj   +1 more source

Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep

open access: yesBMC Veterinary Research, 2021
Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risks and production-limiting diseases in small ruminant populations, particularly in temperate regions.
Jalal Aliabadi   +2 more
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

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