Results 51 to 60 of about 2,923,932 (298)
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
PhosphoThreonine Western Blotting v1
, 2015 This protocol is for pSerine and pThreonine western blotting (optimized for detecting phospho FMRP protein after IP)
core +1 more source
A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma
FEBS Letters, EarlyView.Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos +9 more
wiley +1 more source
Evidence for the existence of Dm0-like Lamin in Sf9 cells
浙江大学学报. 农业与生命科学版, 2015 The nuclear membrane of mammalian cells was composed of inner nuclear membrane, outer nuclear membrane and perinuclear space. The lamina was localized under the nucleoplasm face of inner nuclear membrane. It has been known that the lamina was distributed
Wei Wenqiang, Ji Shaoping, Zhang Yinyan
doaj +1 more source
Antibodies used in this study for IHC, western blotting and dot blotting.
, 2013 Antibodies used in this study for IHC, western blotting and dot blotting.
Natalie Haas (444929) +9 more
core +1 more source
Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis
FEBS Letters, EarlyView.This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng +8 more
wiley +1 more source
Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis
Jornal de Pediatria (Versão em Português), 2016 Objective: To evaluate the Western blotting method for the detection of IgG anti‐Toxoplasma gondii (T. gondii) (IgG‐WB) in the serum of children with suspected congenital toxoplasmosis.
Jaqueline Dario Capobiango +6 more
doaj +1 more source
ELISA and western blotting data
, 2019 Data of in vitro and in vivo experiments from ELISA, western blotting and immunostaining ...
Plinio Casarotto (3992108)
core +1 more source
Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells
FEBS Letters, EarlyView.DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park +8 more
wiley +1 more source
Quantifying cytoskeletal protein interactions with far Western blotting
Frontiers in Molecular BiosciencesThe study of cytoskeletal proteins has been limited by some of their intrinsic properties, such as fixed intracellular localization and insolubility, which limit the implementation of many protein–protein interaction assays.
Marcelo M. Melo +4 more
doaj +1 more source