Gel filtration chromatography [PDF]
, 2011 Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil +2 more
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Subcellular localization of Bombyx mori ribosomal protein S3a and effect of its over-expression on BmNPV infection [PDF]
, 2012 In the present study, using a BV/PH-Bms3a-EGFP, we found that Bombyx mori ribosomal protein S3a (BmS3a) with EGFP fused to its C-terminal, was predominantly localized in the cytoplasm of B. mori cells.
Hui-ling, W +6 more
core +2 more sources
Short hairpin RNA expression for enhancing the resistance of Bombyx mori (Bm) to nucleopolyhedrovirus in vitro and in vivo [PDF]
, 2016 A new paradigm of RNAi technology has been studied for enhancing the resistance to virus in plants and animals. Previous studies have shown that the Bombyx mori (Bm) U6 promoter based shRNA is an effective tool for inducing RNAi in Bombyx mori cell line.
Bhaskar, Roy +5 more
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Bmserpin2 Is Involved in BmNPV Infection by Suppressing Melanization in Bombyx mori [PDF]
Insects, 2019 Melanization, an important defense response, plays a vital role in arthropod immunity. It is mediated by serine proteases (SPs) that convert the inactive prophenoloxidase (PPO) to active phenoloxidase (PO) and is tightly regulated by serine protease inhibitors (serpins) which belong to a well distributed superfamily in invertebrates, participating in ...
Toufeeq, Shahzad +7 more
openaire +3 more sources
Research on expression of HIV-1 env in baculovirus [PDF]
, 2000 昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种，以一种无需额外辅助的重组昆虫杆状病毒作为表达载体，目前已被应用来表达不同来源的基因，如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高，蛋白经过完善的后处理过程，如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因，使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白，目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白；并探索其应用在检测试剂中的前景。Baculovirus ...
郭庆
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Recombinant baculoviruses as vectors for identifying proteins encoded by intron-containing members of complex multigene families [PDF]
, 1989 Using a transfer vector derived from Bombyx mori nuclear polyhedrosis virus (BmNPV), we have constructed recombinant baculoviruses that contain complete silk moth chorion chromosomal genes encoding high-cysteine proteins under the control of the ...
Iatrou, Kostas +2 more
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Advances in the Arms Race Between Silkworm and Baculovirus [PDF]
, 2021 Insects are the largest group of animals. Nearly all organisms, including insects, have viral pathogens. An important domesticated economic insect is the silkworm moth Bombyx mori. B.
Goldsmith, Marian R. +2 more
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Prevalence of White Spot Disease in Penaeus Monodon In Relation To Environmental Changes and the Occurrence of Apoptosis [PDF]
, 2003 White s pot disease (WSD) is an important viral disease of cultured penaeid shrimp. Despite the amount of research that has been carried out on this disease, much still remains to be done.
Sahtout, Abeer Hassan
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Targeting ie-1 gene by RNAi induces baculoviral resistance in lepidopteran cell lines and in transgenic silkworms [PDF]
, 2007 RNA interference (RNAi)-mediated viral inhibition has been used in a few organisms for eliciting viral resistance. In the present study, we report the use of RNAi in preventing baculovirus infection in a lepidopteran.
Chandrashekaraiah, +9 more
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Thermostability of the Recombinant Haemagglutinin-Neuraminidase Glycoproteins of Newcastle Disease Virus [PDF]
, 2003 The haemagglutinin-neuraminidase (HN) glycoprotein of Newcastle disease virus (NDV) is of primary importance in inducing virus-neutralizing antibodies against viral infection in chicken and has been used in the development of many vaccines.
Tang, Yik Kiong
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